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Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town
BACKGROUND: Calf diarrhoea is the most serious issue in the livestock industry, resulting in significant financial losses. METHODS: A study was undertaken in 32 urban and peri‐urban dairy farms of Hawassa town to isolate E. coli from diarrhoeic calves, assess associated putative factors related to t...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959283/ https://www.ncbi.nlm.nih.gov/pubmed/34910854 http://dx.doi.org/10.1002/vms3.686 |
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author | Fesseha, Haben Mathewos, Mesfin Aliye, Saliman Mekonnen, Endale |
author_facet | Fesseha, Haben Mathewos, Mesfin Aliye, Saliman Mekonnen, Endale |
author_sort | Fesseha, Haben |
collection | PubMed |
description | BACKGROUND: Calf diarrhoea is the most serious issue in the livestock industry, resulting in significant financial losses. METHODS: A study was undertaken in 32 urban and peri‐urban dairy farms of Hawassa town to isolate E. coli from diarrhoeic calves, assess associated putative factors related to the occurrence, and the evaluate antibacterial susceptibility patterns of isolates. A convenience sampling technique was performed for the selection of these dairy farms and calf samples. A total of 68 faecal samples were collected directly from the rectum of diarrhoeic calves. The faecal samples were confirmed as E. coli O157: H7 positive using the latex agglutination test. RESULTS: In this study, 47(69.1%) samples were positive for E. coli, of which 22 (46.8%) were identified as E. coli O157:H7 strains based on their latex agglutination character. Factors such as frequency of calf house cleaning, type of supplement provided, and method of colostrum feeding were significantly correlated (p < 0.05) with calf diarrhoea, while the other risk factors had no significant association. Antibiogram of E. coli O157:H7 isolates showed that the isolates were highly sensitive to gentamycin, ceftriaxone, trimethoprim‐sulphamethoxazole, and ciprofloxacin and were found to be resistant to tetracycline, kanamycin and amoxicillin. CONCLUSION: Our findings revealed that calf diarrhoea is still a major health problem of calves in the study area. Hence, improved calf and farm management practice, an ad libitum quantity of colostrum, and good farm hygienic practices should be ensured. This study also revealed that some antibiotic‐resistant E. coli O157:H7 isolates need to be further investigated for their public health implications. |
format | Online Article Text |
id | pubmed-8959283 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89592832022-03-29 Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town Fesseha, Haben Mathewos, Mesfin Aliye, Saliman Mekonnen, Endale Vet Med Sci OTHER BACKGROUND: Calf diarrhoea is the most serious issue in the livestock industry, resulting in significant financial losses. METHODS: A study was undertaken in 32 urban and peri‐urban dairy farms of Hawassa town to isolate E. coli from diarrhoeic calves, assess associated putative factors related to the occurrence, and the evaluate antibacterial susceptibility patterns of isolates. A convenience sampling technique was performed for the selection of these dairy farms and calf samples. A total of 68 faecal samples were collected directly from the rectum of diarrhoeic calves. The faecal samples were confirmed as E. coli O157: H7 positive using the latex agglutination test. RESULTS: In this study, 47(69.1%) samples were positive for E. coli, of which 22 (46.8%) were identified as E. coli O157:H7 strains based on their latex agglutination character. Factors such as frequency of calf house cleaning, type of supplement provided, and method of colostrum feeding were significantly correlated (p < 0.05) with calf diarrhoea, while the other risk factors had no significant association. Antibiogram of E. coli O157:H7 isolates showed that the isolates were highly sensitive to gentamycin, ceftriaxone, trimethoprim‐sulphamethoxazole, and ciprofloxacin and were found to be resistant to tetracycline, kanamycin and amoxicillin. CONCLUSION: Our findings revealed that calf diarrhoea is still a major health problem of calves in the study area. Hence, improved calf and farm management practice, an ad libitum quantity of colostrum, and good farm hygienic practices should be ensured. This study also revealed that some antibiotic‐resistant E. coli O157:H7 isolates need to be further investigated for their public health implications. John Wiley and Sons Inc. 2021-12-15 /pmc/articles/PMC8959283/ /pubmed/34910854 http://dx.doi.org/10.1002/vms3.686 Text en © 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | OTHER Fesseha, Haben Mathewos, Mesfin Aliye, Saliman Mekonnen, Endale Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town |
title | Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town |
title_full | Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town |
title_fullStr | Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town |
title_full_unstemmed | Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town |
title_short | Isolation and antibiogram of Escherichia coli O157: H7 from diarrhoeic calves in urban and peri‐urban dairy farms of Hawassa town |
title_sort | isolation and antibiogram of escherichia coli o157: h7 from diarrhoeic calves in urban and peri‐urban dairy farms of hawassa town |
topic | OTHER |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959283/ https://www.ncbi.nlm.nih.gov/pubmed/34910854 http://dx.doi.org/10.1002/vms3.686 |
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