Cargando…
Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model
Arctigenin (ACT) is a novel anti‐inflammatory lignan extracted from Arctium lappa L, a herb commonly used in traditional Chinese herbal medicine. In this study, we investigated the molecular mechanism whereby ACT inhibits PCV2 infection‐induced proinflammatory cytokine production in vitro and in viv...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959337/ https://www.ncbi.nlm.nih.gov/pubmed/34914190 http://dx.doi.org/10.1002/vms3.693 |
_version_ | 1784677131207311360 |
---|---|
author | Wu, Lijun Chen, Jie Zhou, Danna Chen, Runshan Chen, Xiabing Shao, Zhiyong Yang, Wenhai He, Bin |
author_facet | Wu, Lijun Chen, Jie Zhou, Danna Chen, Runshan Chen, Xiabing Shao, Zhiyong Yang, Wenhai He, Bin |
author_sort | Wu, Lijun |
collection | PubMed |
description | Arctigenin (ACT) is a novel anti‐inflammatory lignan extracted from Arctium lappa L, a herb commonly used in traditional Chinese herbal medicine. In this study, we investigated the molecular mechanism whereby ACT inhibits PCV2 infection‐induced proinflammatory cytokine production in vitro and in vivo. We observed that in PCV2 infection+ACT treated PK‐15 cells, proinflammatory cytokine production was significantly reduced, compared to the PCV2‐infected cells. The transfection and luciferase reporter assay confirmed that ACT suppressed NF‐κB signalling pathway activation following PCV2 infection in PK‐15 cells. Furthermore, western blotting demonstrated that ACT suppressed the NF‐κB signal pathway in PCV2 infection‐stimulated PK‐15 cells by inhibiting the translocation of p65 from the cytoplasm to the nucleus and IκBα phosphorylation. BALB/c mice were used as a model to evaluate the anti‐inflammatory effect of ACT in vivo. We found that the BALB/c mice inoculated with PCV2 infection + ACT treated showed a significant reduction of proinflammatory cytokine production in serum, lung and spleen tissue, compared to the PCV2‐infected mice. Western blotting confirmed that ACT suppressed the NF‐κB signal pathway in PCV2‐infected mice by inhibiting the translocation of p65 from the cytoplasm to the nucleus and IκBα phosphorylation in lung tissue. Our studies first demonstrate that ACT inhibits PCV2 infection‐induced proinflammatory cytokine production by suppressing the phosphorylation and nuclear translocation of NF‐κB in vitro and in vivo. These results will help further develop ACT as a Traditional Chinese herbal medicine remedy in the treatment of porcine circovirus‐associated diseases. |
format | Online Article Text |
id | pubmed-8959337 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89593372022-03-29 Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model Wu, Lijun Chen, Jie Zhou, Danna Chen, Runshan Chen, Xiabing Shao, Zhiyong Yang, Wenhai He, Bin Vet Med Sci REPTILES Arctigenin (ACT) is a novel anti‐inflammatory lignan extracted from Arctium lappa L, a herb commonly used in traditional Chinese herbal medicine. In this study, we investigated the molecular mechanism whereby ACT inhibits PCV2 infection‐induced proinflammatory cytokine production in vitro and in vivo. We observed that in PCV2 infection+ACT treated PK‐15 cells, proinflammatory cytokine production was significantly reduced, compared to the PCV2‐infected cells. The transfection and luciferase reporter assay confirmed that ACT suppressed NF‐κB signalling pathway activation following PCV2 infection in PK‐15 cells. Furthermore, western blotting demonstrated that ACT suppressed the NF‐κB signal pathway in PCV2 infection‐stimulated PK‐15 cells by inhibiting the translocation of p65 from the cytoplasm to the nucleus and IκBα phosphorylation. BALB/c mice were used as a model to evaluate the anti‐inflammatory effect of ACT in vivo. We found that the BALB/c mice inoculated with PCV2 infection + ACT treated showed a significant reduction of proinflammatory cytokine production in serum, lung and spleen tissue, compared to the PCV2‐infected mice. Western blotting confirmed that ACT suppressed the NF‐κB signal pathway in PCV2‐infected mice by inhibiting the translocation of p65 from the cytoplasm to the nucleus and IκBα phosphorylation in lung tissue. Our studies first demonstrate that ACT inhibits PCV2 infection‐induced proinflammatory cytokine production by suppressing the phosphorylation and nuclear translocation of NF‐κB in vitro and in vivo. These results will help further develop ACT as a Traditional Chinese herbal medicine remedy in the treatment of porcine circovirus‐associated diseases. John Wiley and Sons Inc. 2021-12-16 /pmc/articles/PMC8959337/ /pubmed/34914190 http://dx.doi.org/10.1002/vms3.693 Text en © 2021 The Authors. Veterinary Medicine and Science published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | REPTILES Wu, Lijun Chen, Jie Zhou, Danna Chen, Runshan Chen, Xiabing Shao, Zhiyong Yang, Wenhai He, Bin Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model |
title | Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model |
title_full | Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model |
title_fullStr | Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model |
title_full_unstemmed | Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model |
title_short | Anti‐inflammatory activity of arctigenin against PCV2 infection in a mouse model |
title_sort | anti‐inflammatory activity of arctigenin against pcv2 infection in a mouse model |
topic | REPTILES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959337/ https://www.ncbi.nlm.nih.gov/pubmed/34914190 http://dx.doi.org/10.1002/vms3.693 |
work_keys_str_mv | AT wulijun antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel AT chenjie antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel AT zhoudanna antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel AT chenrunshan antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel AT chenxiabing antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel AT shaozhiyong antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel AT yangwenhai antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel AT hebin antiinflammatoryactivityofarctigeninagainstpcv2infectioninamousemodel |