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Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets
Newborn mammals, including piglets, exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). The electrophysiologic properties of this naturally regenerated myocardium have not been examined. We hypothesized that e...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959497/ https://www.ncbi.nlm.nih.gov/pubmed/35355973 http://dx.doi.org/10.3389/fcvm.2022.829546 |
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author | Wang, Hanjay Pong, Terrence Obafemi, Oluwatomisin O. Lucian, Haley J. Aparicio-Valenzuela, Joy Tran, Nicholas A. Mullis, Danielle M. Elde, Stefan Tada, Yuko Baker, Sam W. Wang, Caroline Y. Cyr, Kevin J. Paulsen, Michael J. Zhu, Yuanjia Lee, Anson M. Woo, Y. Joseph |
author_facet | Wang, Hanjay Pong, Terrence Obafemi, Oluwatomisin O. Lucian, Haley J. Aparicio-Valenzuela, Joy Tran, Nicholas A. Mullis, Danielle M. Elde, Stefan Tada, Yuko Baker, Sam W. Wang, Caroline Y. Cyr, Kevin J. Paulsen, Michael J. Zhu, Yuanjia Lee, Anson M. Woo, Y. Joseph |
author_sort | Wang, Hanjay |
collection | PubMed |
description | Newborn mammals, including piglets, exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). The electrophysiologic properties of this naturally regenerated myocardium have not been examined. We hypothesized that epicardial conduction is preserved after P1 MI in piglets. Yorkshire-Landrace piglets underwent left anterior descending coronary artery ligation at age P1 (n = 6) or P7 (n = 7), After 7 weeks, cardiac magnetic resonance imaging was performed with late gadolinium enhancement for analysis of fibrosis. Epicardial conduction mapping was performed using custom 3D-printed high-resolution mapping arrays. Age- and weight-matched healthy pigs served as controls (n = 6). At the study endpoint, left ventricular (LV) ejection fraction was similar for controls and P1 pigs (46.4 ± 3.0% vs. 40.3 ± 4.9%, p = 0.132), but significantly depressed for P7 pigs (30.2 ± 6.6%, p < 0.001 vs. control). The percentage of LV myocardial volume consisting of fibrotic scar was 1.0 ± 0.4% in controls, 9.9 ± 4.4% in P1 pigs (p = 0.002 vs. control), and 17.3 ± 4.6% in P7 pigs (p < 0.001 vs. control, p = 0.007 vs. P1). Isochrone activation maps and apex activation time were similar between controls and P1 pigs (9.4 ± 1.6 vs. 7.8 ± 0.9 ms, p = 0.649), but significantly prolonged in P7 pigs (21.3 ± 5.1 ms, p < 0.001 vs. control, p < 0.001 vs. P1). Conduction velocity was similar between controls and P1 pigs (1.0 ± 0.2 vs. 1.1 ± 0.4 mm/ms, p = 0.852), but slower in P7 pigs (0.7 ± 0.2 mm/ms, p = 0.129 vs. control, p = 0.052 vs. P1). Overall, our data suggest that epicardial conduction dynamics are conserved in the setting of natural heart regeneration in piglets after P1 MI. |
format | Online Article Text |
id | pubmed-8959497 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89594972022-03-29 Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets Wang, Hanjay Pong, Terrence Obafemi, Oluwatomisin O. Lucian, Haley J. Aparicio-Valenzuela, Joy Tran, Nicholas A. Mullis, Danielle M. Elde, Stefan Tada, Yuko Baker, Sam W. Wang, Caroline Y. Cyr, Kevin J. Paulsen, Michael J. Zhu, Yuanjia Lee, Anson M. Woo, Y. Joseph Front Cardiovasc Med Cardiovascular Medicine Newborn mammals, including piglets, exhibit natural heart regeneration after myocardial infarction (MI) on postnatal day 1 (P1), but this ability is lost by postnatal day 7 (P7). The electrophysiologic properties of this naturally regenerated myocardium have not been examined. We hypothesized that epicardial conduction is preserved after P1 MI in piglets. Yorkshire-Landrace piglets underwent left anterior descending coronary artery ligation at age P1 (n = 6) or P7 (n = 7), After 7 weeks, cardiac magnetic resonance imaging was performed with late gadolinium enhancement for analysis of fibrosis. Epicardial conduction mapping was performed using custom 3D-printed high-resolution mapping arrays. Age- and weight-matched healthy pigs served as controls (n = 6). At the study endpoint, left ventricular (LV) ejection fraction was similar for controls and P1 pigs (46.4 ± 3.0% vs. 40.3 ± 4.9%, p = 0.132), but significantly depressed for P7 pigs (30.2 ± 6.6%, p < 0.001 vs. control). The percentage of LV myocardial volume consisting of fibrotic scar was 1.0 ± 0.4% in controls, 9.9 ± 4.4% in P1 pigs (p = 0.002 vs. control), and 17.3 ± 4.6% in P7 pigs (p < 0.001 vs. control, p = 0.007 vs. P1). Isochrone activation maps and apex activation time were similar between controls and P1 pigs (9.4 ± 1.6 vs. 7.8 ± 0.9 ms, p = 0.649), but significantly prolonged in P7 pigs (21.3 ± 5.1 ms, p < 0.001 vs. control, p < 0.001 vs. P1). Conduction velocity was similar between controls and P1 pigs (1.0 ± 0.2 vs. 1.1 ± 0.4 mm/ms, p = 0.852), but slower in P7 pigs (0.7 ± 0.2 mm/ms, p = 0.129 vs. control, p = 0.052 vs. P1). Overall, our data suggest that epicardial conduction dynamics are conserved in the setting of natural heart regeneration in piglets after P1 MI. Frontiers Media S.A. 2022-03-09 /pmc/articles/PMC8959497/ /pubmed/35355973 http://dx.doi.org/10.3389/fcvm.2022.829546 Text en Copyright © 2022 Wang, Pong, Obafemi, Lucian, Aparicio-Valenzuela, Tran, Mullis, Elde, Tada, Baker, Wang, Cyr, Paulsen, Zhu, Lee and Woo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cardiovascular Medicine Wang, Hanjay Pong, Terrence Obafemi, Oluwatomisin O. Lucian, Haley J. Aparicio-Valenzuela, Joy Tran, Nicholas A. Mullis, Danielle M. Elde, Stefan Tada, Yuko Baker, Sam W. Wang, Caroline Y. Cyr, Kevin J. Paulsen, Michael J. Zhu, Yuanjia Lee, Anson M. Woo, Y. Joseph Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets |
title | Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets |
title_full | Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets |
title_fullStr | Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets |
title_full_unstemmed | Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets |
title_short | Electrophysiologic Conservation of Epicardial Conduction Dynamics After Myocardial Infarction and Natural Heart Regeneration in Newborn Piglets |
title_sort | electrophysiologic conservation of epicardial conduction dynamics after myocardial infarction and natural heart regeneration in newborn piglets |
topic | Cardiovascular Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959497/ https://www.ncbi.nlm.nih.gov/pubmed/35355973 http://dx.doi.org/10.3389/fcvm.2022.829546 |
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