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Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE
INTRODUCTION: Trypanosomosis is an important disease of dromedary camels caused by the pathogenic protozoan Trypanosoma evansi. This study aimed to compare three different tests for its diagnosis in this species: conventional microscopy, the card agglutination test for trypanosomosis/T. evansi (CATT...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959682/ https://www.ncbi.nlm.nih.gov/pubmed/35582483 http://dx.doi.org/10.2478/jvetres-2022-0002 |
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author | Habeeba, Shameem Khan, Rashid Ali Zackaria, Hassan Yammahi, Saeed Mohamed, Zulaikha Sobhi, Wissam AbdelKader, Ayman Alhosani, Mohamed Ali Muhairi, Salama Al |
author_facet | Habeeba, Shameem Khan, Rashid Ali Zackaria, Hassan Yammahi, Saeed Mohamed, Zulaikha Sobhi, Wissam AbdelKader, Ayman Alhosani, Mohamed Ali Muhairi, Salama Al |
author_sort | Habeeba, Shameem |
collection | PubMed |
description | INTRODUCTION: Trypanosomosis is an important disease of dromedary camels caused by the pathogenic protozoan Trypanosoma evansi. This study aimed to compare three different tests for its diagnosis in this species: conventional microscopy, the card agglutination test for trypanosomosis/T. evansi (CATT/T. evansi) and real-time PCR. MATERIAL AND METHODS: Whole blood and serum samples collected from 77 dromedary camels of Abu Dhabi, United Arab Emirates, were analysed with the test methods stated. Statistical analysis was done using McNemar’s chi-squared test, and Cohen’s kappa index (κ) was calculated. RESULTS: We obtained results with positivity of 18% (14/77) by microscopy, 22% by CATT (17/77) and 60% (46/77) by real-time PCR, with the chain reaction detecting at a respectively three- and two-fold greater rate than the other techniques. Analysis of the data revealed a relative sensitivity of 30.4% and 37.0% for microscopy and CATT, respectively, compared to real-time PCR. The difference between the real-time PCR’s sensitivity and those of the other methods was statistically significant, with X(2) values of 30.03 and 20.1, respectively (df = 1 and P = 0.05 in both cases). Agreement of microscopy results with those of with CATT was good (κ = 0.72; 95% CI = 0.62–0.82). Cohen’s kappa index showed fair agreement of real-time PCR with microscopy (κ = 0.26; 95% CI = 0.16–0.36) whereas it was in poor agreement with CATT (κ = 0.09; 95% CI = 0.02–0.15). CONCLUSION: Real-time PCR was found to be more sensitive than microscopy and CATT. |
format | Online Article Text |
id | pubmed-8959682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-89596822022-05-16 Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE Habeeba, Shameem Khan, Rashid Ali Zackaria, Hassan Yammahi, Saeed Mohamed, Zulaikha Sobhi, Wissam AbdelKader, Ayman Alhosani, Mohamed Ali Muhairi, Salama Al J Vet Res Research Article INTRODUCTION: Trypanosomosis is an important disease of dromedary camels caused by the pathogenic protozoan Trypanosoma evansi. This study aimed to compare three different tests for its diagnosis in this species: conventional microscopy, the card agglutination test for trypanosomosis/T. evansi (CATT/T. evansi) and real-time PCR. MATERIAL AND METHODS: Whole blood and serum samples collected from 77 dromedary camels of Abu Dhabi, United Arab Emirates, were analysed with the test methods stated. Statistical analysis was done using McNemar’s chi-squared test, and Cohen’s kappa index (κ) was calculated. RESULTS: We obtained results with positivity of 18% (14/77) by microscopy, 22% by CATT (17/77) and 60% (46/77) by real-time PCR, with the chain reaction detecting at a respectively three- and two-fold greater rate than the other techniques. Analysis of the data revealed a relative sensitivity of 30.4% and 37.0% for microscopy and CATT, respectively, compared to real-time PCR. The difference between the real-time PCR’s sensitivity and those of the other methods was statistically significant, with X(2) values of 30.03 and 20.1, respectively (df = 1 and P = 0.05 in both cases). Agreement of microscopy results with those of with CATT was good (κ = 0.72; 95% CI = 0.62–0.82). Cohen’s kappa index showed fair agreement of real-time PCR with microscopy (κ = 0.26; 95% CI = 0.16–0.36) whereas it was in poor agreement with CATT (κ = 0.09; 95% CI = 0.02–0.15). CONCLUSION: Real-time PCR was found to be more sensitive than microscopy and CATT. Sciendo 2022-03-25 /pmc/articles/PMC8959682/ /pubmed/35582483 http://dx.doi.org/10.2478/jvetres-2022-0002 Text en © 2022 S. Habeeba et al., published by Sciendo https://creativecommons.org/licenses/by-nc-nd/3.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License. |
spellingShingle | Research Article Habeeba, Shameem Khan, Rashid Ali Zackaria, Hassan Yammahi, Saeed Mohamed, Zulaikha Sobhi, Wissam AbdelKader, Ayman Alhosani, Mohamed Ali Muhairi, Salama Al Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE |
title | Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE |
title_full | Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE |
title_fullStr | Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE |
title_full_unstemmed | Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE |
title_short | Comparison of Microscopy, Card Agglutination Test for Trypanosoma Evansi, and Real-time PCR in The Diagnosis of Trypanosomosis in Dromedary Camels of The Abu Dhabi Emirate, UAE |
title_sort | comparison of microscopy, card agglutination test for trypanosoma evansi, and real-time pcr in the diagnosis of trypanosomosis in dromedary camels of the abu dhabi emirate, uae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959682/ https://www.ncbi.nlm.nih.gov/pubmed/35582483 http://dx.doi.org/10.2478/jvetres-2022-0002 |
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