Cargando…
A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576
Sophorolipids (SLs), an important biosurfactant produced by S. bombicola, were one of the most potential substitutes for chemical surfactants. Few reports on the transcriptional regulation of SLs synthesis and the engineered strains with high-yield SLs were available. In this study, a Rim9-like prot...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959766/ https://www.ncbi.nlm.nih.gov/pubmed/35356780 http://dx.doi.org/10.3389/fbioe.2022.818445 |
_version_ | 1784677231891578880 |
---|---|
author | Liu, Jun Zhang, Xinyu Liu, Guodong Zhao, Guoqin Fang, Xiaoran Song, Xin |
author_facet | Liu, Jun Zhang, Xinyu Liu, Guodong Zhao, Guoqin Fang, Xiaoran Song, Xin |
author_sort | Liu, Jun |
collection | PubMed |
description | Sophorolipids (SLs), an important biosurfactant produced by S. bombicola, were one of the most potential substitutes for chemical surfactants. Few reports on the transcriptional regulation of SLs synthesis and the engineered strains with high-yield SLs were available. In this study, a Rim9-like protein (Rlp) and three transcription factors (ztf1, leu3, gcl) were mined and analyzed, and a progressive enhancement of SLs production was achieved through cumulative knockouts of three genes. The sophorolipid production of ΔrlpΔleu3Δztf1 reached 97.44 g/L, increased by 50.51% than that of the wild-type strain. Compared with the wild-type strain, the flow of glucose to SLs synthesis pathways was increased, and the synthesis of branched-chain amino acids was reduced in ΔrlpΔleu3Δztf1. The amount of UDP-glucose, the substrate for two glycosyltransferases, also increased, and the expression level of the key genes sble and UGPase for SLs synthesis increased by 2.2 times, respectively. The multiple-gene knockout strategy was proved to be highly effective to construct the engineered strain with high-yield SLs production, and this strain was a superior strain for industrial fermentation of SLs and reduced SLs production costs. |
format | Online Article Text |
id | pubmed-8959766 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89597662022-03-29 A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576 Liu, Jun Zhang, Xinyu Liu, Guodong Zhao, Guoqin Fang, Xiaoran Song, Xin Front Bioeng Biotechnol Bioengineering and Biotechnology Sophorolipids (SLs), an important biosurfactant produced by S. bombicola, were one of the most potential substitutes for chemical surfactants. Few reports on the transcriptional regulation of SLs synthesis and the engineered strains with high-yield SLs were available. In this study, a Rim9-like protein (Rlp) and three transcription factors (ztf1, leu3, gcl) were mined and analyzed, and a progressive enhancement of SLs production was achieved through cumulative knockouts of three genes. The sophorolipid production of ΔrlpΔleu3Δztf1 reached 97.44 g/L, increased by 50.51% than that of the wild-type strain. Compared with the wild-type strain, the flow of glucose to SLs synthesis pathways was increased, and the synthesis of branched-chain amino acids was reduced in ΔrlpΔleu3Δztf1. The amount of UDP-glucose, the substrate for two glycosyltransferases, also increased, and the expression level of the key genes sble and UGPase for SLs synthesis increased by 2.2 times, respectively. The multiple-gene knockout strategy was proved to be highly effective to construct the engineered strain with high-yield SLs production, and this strain was a superior strain for industrial fermentation of SLs and reduced SLs production costs. Frontiers Media S.A. 2022-03-09 /pmc/articles/PMC8959766/ /pubmed/35356780 http://dx.doi.org/10.3389/fbioe.2022.818445 Text en Copyright © 2022 Liu, Zhang, Liu, Zhao, Fang and Song. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Liu, Jun Zhang, Xinyu Liu, Guodong Zhao, Guoqin Fang, Xiaoran Song, Xin A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576 |
title | A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576 |
title_full | A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576 |
title_fullStr | A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576 |
title_full_unstemmed | A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576 |
title_short | A Cumulative Effect by Multiple-Gene Knockout Strategy Leads to a Significant Increase in the Production of Sophorolipids in Starmerella Bombicola CGMCC 1576 |
title_sort | cumulative effect by multiple-gene knockout strategy leads to a significant increase in the production of sophorolipids in starmerella bombicola cgmcc 1576 |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959766/ https://www.ncbi.nlm.nih.gov/pubmed/35356780 http://dx.doi.org/10.3389/fbioe.2022.818445 |
work_keys_str_mv | AT liujun acumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT zhangxinyu acumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT liuguodong acumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT zhaoguoqin acumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT fangxiaoran acumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT songxin acumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT liujun cumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT zhangxinyu cumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT liuguodong cumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT zhaoguoqin cumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT fangxiaoran cumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 AT songxin cumulativeeffectbymultiplegeneknockoutstrategyleadstoasignificantincreaseintheproductionofsophorolipidsinstarmerellabombicolacgmcc1576 |