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Reticulocyte Maturation and Variant Red Blood Cells

The bone marrow produces billions of reticulocytes daily. These reticulocytes mature into red blood cells by reducing their plasma membrane by 20% and ejecting or degrading residual internal organelles, membranes and proteins not required by the mature cell. This process occurs by autophagy, protein...

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Autores principales: Stevens-Hernandez, Christian J., Flatt, Joanna F., Kupzig, Sabine, Bruce, Lesley J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959883/
https://www.ncbi.nlm.nih.gov/pubmed/35356079
http://dx.doi.org/10.3389/fphys.2022.834463
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author Stevens-Hernandez, Christian J.
Flatt, Joanna F.
Kupzig, Sabine
Bruce, Lesley J.
author_facet Stevens-Hernandez, Christian J.
Flatt, Joanna F.
Kupzig, Sabine
Bruce, Lesley J.
author_sort Stevens-Hernandez, Christian J.
collection PubMed
description The bone marrow produces billions of reticulocytes daily. These reticulocytes mature into red blood cells by reducing their plasma membrane by 20% and ejecting or degrading residual internal organelles, membranes and proteins not required by the mature cell. This process occurs by autophagy, protein degradation and vesiculation but is not well understood. We previously reported that Southeast Asian Ovalocytic RBCs demonstrate incomplete reticulocyte maturation and we have now extended this study to a number of other variant RBCs. By comparing the profile of a pure reticulocyte preparation of cultured red cells with these variant cells, we show that the largest of these cells, the overhydrated hereditary stomatocytosis cells, are the least mature, they barely reduced their plasma membrane and contain large amounts of proteins that should have been reduced or removed. Intermediate sized variant RBCs appear to be more mature but retain some endoplasmic reticulum and residual membrane proteins. We propose that the size and composition of these variant cell types correlate with the different stages of reticulocyte maturation and provide insight into the reticulocyte maturation process.
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spelling pubmed-89598832022-03-29 Reticulocyte Maturation and Variant Red Blood Cells Stevens-Hernandez, Christian J. Flatt, Joanna F. Kupzig, Sabine Bruce, Lesley J. Front Physiol Physiology The bone marrow produces billions of reticulocytes daily. These reticulocytes mature into red blood cells by reducing their plasma membrane by 20% and ejecting or degrading residual internal organelles, membranes and proteins not required by the mature cell. This process occurs by autophagy, protein degradation and vesiculation but is not well understood. We previously reported that Southeast Asian Ovalocytic RBCs demonstrate incomplete reticulocyte maturation and we have now extended this study to a number of other variant RBCs. By comparing the profile of a pure reticulocyte preparation of cultured red cells with these variant cells, we show that the largest of these cells, the overhydrated hereditary stomatocytosis cells, are the least mature, they barely reduced their plasma membrane and contain large amounts of proteins that should have been reduced or removed. Intermediate sized variant RBCs appear to be more mature but retain some endoplasmic reticulum and residual membrane proteins. We propose that the size and composition of these variant cell types correlate with the different stages of reticulocyte maturation and provide insight into the reticulocyte maturation process. Frontiers Media S.A. 2022-03-07 /pmc/articles/PMC8959883/ /pubmed/35356079 http://dx.doi.org/10.3389/fphys.2022.834463 Text en Copyright © 2022 Stevens-Hernandez, Flatt, Kupzig and Bruce. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Stevens-Hernandez, Christian J.
Flatt, Joanna F.
Kupzig, Sabine
Bruce, Lesley J.
Reticulocyte Maturation and Variant Red Blood Cells
title Reticulocyte Maturation and Variant Red Blood Cells
title_full Reticulocyte Maturation and Variant Red Blood Cells
title_fullStr Reticulocyte Maturation and Variant Red Blood Cells
title_full_unstemmed Reticulocyte Maturation and Variant Red Blood Cells
title_short Reticulocyte Maturation and Variant Red Blood Cells
title_sort reticulocyte maturation and variant red blood cells
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959883/
https://www.ncbi.nlm.nih.gov/pubmed/35356079
http://dx.doi.org/10.3389/fphys.2022.834463
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