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Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish

The pretectum has a distinct nuclear arrangement and complex neurochemical anatomy. While previous genoarchitectural studies have described rostrocaudal and dorsoventral progenitor domains and subdomains in different species, the relationship between these early partitions and its later derivatives...

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Autores principales: Brożko, Nikola, Baggio, Suelen, Lipiec, Marcin A., Jankowska, Marta, Szewczyk, Łukasz M., Gabriel, Michael O., Chakraborty, Chaitali, Ferran, José L., Wiśniewska, Marta B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959918/
https://www.ncbi.nlm.nih.gov/pubmed/35356436
http://dx.doi.org/10.3389/fnana.2022.838567
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author Brożko, Nikola
Baggio, Suelen
Lipiec, Marcin A.
Jankowska, Marta
Szewczyk, Łukasz M.
Gabriel, Michael O.
Chakraborty, Chaitali
Ferran, José L.
Wiśniewska, Marta B.
author_facet Brożko, Nikola
Baggio, Suelen
Lipiec, Marcin A.
Jankowska, Marta
Szewczyk, Łukasz M.
Gabriel, Michael O.
Chakraborty, Chaitali
Ferran, José L.
Wiśniewska, Marta B.
author_sort Brożko, Nikola
collection PubMed
description The pretectum has a distinct nuclear arrangement and complex neurochemical anatomy. While previous genoarchitectural studies have described rostrocaudal and dorsoventral progenitor domains and subdomains in different species, the relationship between these early partitions and its later derivatives in the mature anatomy is less understood. The signals and transcription factors that control the establishment of pretectal anatomy are practically unknown. We investigated the possibility that some aspects of the development of pretectal divisions are controlled by Wnt signaling, focusing on the transitional stage between neurogenesis and histogenesis in zebrafish. Using several molecular markers and following the prosomeric model, we identified derivatives from each rostrocaudal pretectal progenitor domain and described the localization of gad1b-positive GABAergic and vglut2.2-positive glutamatergic cell clusters. We also attempted to relate these clusters to pretectal nuclei in the mature brain. Then, we examined the influence of Wnt signaling on the size of neurochemically distinctive pretectal areas, using a chemical inhibitor of the Wnt pathway and the CRISPR/Cas9 approach to knock out genes that encode the Wnt pathway mediators, Lef1 and Tcf7l2. The downregulation of the Wnt pathway led to a decrease in two GABAergic clusters and an expansion of a glutamatergic subregion in the maturing pretectum. This revealed an instructive role of the Wnt signal in the development of the pretectum during neurogenesis. The molecular anatomy presented here improves our understanding of pretectal development during early postmitotic stages and support the hypothesis that Wnt signaling is involved in shaping the neurochemical organization of the pretectum.
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spelling pubmed-89599182022-03-29 Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish Brożko, Nikola Baggio, Suelen Lipiec, Marcin A. Jankowska, Marta Szewczyk, Łukasz M. Gabriel, Michael O. Chakraborty, Chaitali Ferran, José L. Wiśniewska, Marta B. Front Neuroanat Neuroanatomy The pretectum has a distinct nuclear arrangement and complex neurochemical anatomy. While previous genoarchitectural studies have described rostrocaudal and dorsoventral progenitor domains and subdomains in different species, the relationship between these early partitions and its later derivatives in the mature anatomy is less understood. The signals and transcription factors that control the establishment of pretectal anatomy are practically unknown. We investigated the possibility that some aspects of the development of pretectal divisions are controlled by Wnt signaling, focusing on the transitional stage between neurogenesis and histogenesis in zebrafish. Using several molecular markers and following the prosomeric model, we identified derivatives from each rostrocaudal pretectal progenitor domain and described the localization of gad1b-positive GABAergic and vglut2.2-positive glutamatergic cell clusters. We also attempted to relate these clusters to pretectal nuclei in the mature brain. Then, we examined the influence of Wnt signaling on the size of neurochemically distinctive pretectal areas, using a chemical inhibitor of the Wnt pathway and the CRISPR/Cas9 approach to knock out genes that encode the Wnt pathway mediators, Lef1 and Tcf7l2. The downregulation of the Wnt pathway led to a decrease in two GABAergic clusters and an expansion of a glutamatergic subregion in the maturing pretectum. This revealed an instructive role of the Wnt signal in the development of the pretectum during neurogenesis. The molecular anatomy presented here improves our understanding of pretectal development during early postmitotic stages and support the hypothesis that Wnt signaling is involved in shaping the neurochemical organization of the pretectum. Frontiers Media S.A. 2022-03-09 /pmc/articles/PMC8959918/ /pubmed/35356436 http://dx.doi.org/10.3389/fnana.2022.838567 Text en Copyright © 2022 Brożko, Baggio, Lipiec, Jankowska, Szewczyk, Gabriel, Chakraborty, Ferran and Wiśniewska. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroanatomy
Brożko, Nikola
Baggio, Suelen
Lipiec, Marcin A.
Jankowska, Marta
Szewczyk, Łukasz M.
Gabriel, Michael O.
Chakraborty, Chaitali
Ferran, José L.
Wiśniewska, Marta B.
Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish
title Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish
title_full Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish
title_fullStr Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish
title_full_unstemmed Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish
title_short Genoarchitecture of the Early Postmitotic Pretectum and the Role of Wnt Signaling in Shaping Pretectal Neurochemical Anatomy in Zebrafish
title_sort genoarchitecture of the early postmitotic pretectum and the role of wnt signaling in shaping pretectal neurochemical anatomy in zebrafish
topic Neuroanatomy
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8959918/
https://www.ncbi.nlm.nih.gov/pubmed/35356436
http://dx.doi.org/10.3389/fnana.2022.838567
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