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CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251
Chikungunya virus (CHIKV), a (re)emerging arbovirus, is the causative agent of chikungunya fever. To date, no approved vaccine or specific antiviral therapy are available. CHIKV has repeatedly been responsible for serious economic and public health impacts in countries where CHIKV epidemics occurred...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960095/ https://www.ncbi.nlm.nih.gov/pubmed/35347588 http://dx.doi.org/10.1007/s11262-022-01892-x |
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author | Hucke, Friederike I. L. Bestehorn-Willmann, Malena Bassetto, Marcella Brancale, Andrea Zanetta, Paola Bugert, Joachim J. |
author_facet | Hucke, Friederike I. L. Bestehorn-Willmann, Malena Bassetto, Marcella Brancale, Andrea Zanetta, Paola Bugert, Joachim J. |
author_sort | Hucke, Friederike I. L. |
collection | PubMed |
description | Chikungunya virus (CHIKV), a (re)emerging arbovirus, is the causative agent of chikungunya fever. To date, no approved vaccine or specific antiviral therapy are available. CHIKV has repeatedly been responsible for serious economic and public health impacts in countries where CHIKV epidemics occurred. Antiviral tests in vitro are generally performed in Vero-B4 cells, a well characterised cell line derived from the kidney of an African green monkey. In this work we characterised a CHIKV patient isolate from Brazil (CHIKV(Brazil)) with regard to cell affinity, infectivity, propagation and cell damage and compared it with a high-passage lab strain (CHIKV(Ross)). Infecting various cell lines (Vero-B4, A549, Huh-7, DBTRG, U251, and U138) with both virus strains, we found distinct differences between the two viruses. CHIKV(Brazil) does not cause cytopathic effects (CPE) in the human hepatocarcinoma cell line Huh-7. Neither CHIKV(Brazil) nor CHIKV(Ross) caused CPE on A549 human lung epithelial cells. The human astrocyte derived glioblastoma cell lines U138 and U251 were found to be effective models for lytic infection with both virus strains and we discuss their predictive potential for neurogenic CHIKV disease. We also detected significant differences in antiviral efficacies regarding the two CHIKV strains. Generally, the antivirals ribavirin, hydroxychloroquine (HCQ) and T-1105 seem to work better against CHIKV(Brazil) in glioblastoma cells than in Vero-B4. Finally, full genome analyses of the CHIKV isolates were done in order to determine their lineage and possibly explain differences in tissue range and antiviral compound efficacies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11262-022-01892-x. |
format | Online Article Text |
id | pubmed-8960095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-89600952022-03-29 CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251 Hucke, Friederike I. L. Bestehorn-Willmann, Malena Bassetto, Marcella Brancale, Andrea Zanetta, Paola Bugert, Joachim J. Virus Genes Original Paper Chikungunya virus (CHIKV), a (re)emerging arbovirus, is the causative agent of chikungunya fever. To date, no approved vaccine or specific antiviral therapy are available. CHIKV has repeatedly been responsible for serious economic and public health impacts in countries where CHIKV epidemics occurred. Antiviral tests in vitro are generally performed in Vero-B4 cells, a well characterised cell line derived from the kidney of an African green monkey. In this work we characterised a CHIKV patient isolate from Brazil (CHIKV(Brazil)) with regard to cell affinity, infectivity, propagation and cell damage and compared it with a high-passage lab strain (CHIKV(Ross)). Infecting various cell lines (Vero-B4, A549, Huh-7, DBTRG, U251, and U138) with both virus strains, we found distinct differences between the two viruses. CHIKV(Brazil) does not cause cytopathic effects (CPE) in the human hepatocarcinoma cell line Huh-7. Neither CHIKV(Brazil) nor CHIKV(Ross) caused CPE on A549 human lung epithelial cells. The human astrocyte derived glioblastoma cell lines U138 and U251 were found to be effective models for lytic infection with both virus strains and we discuss their predictive potential for neurogenic CHIKV disease. We also detected significant differences in antiviral efficacies regarding the two CHIKV strains. Generally, the antivirals ribavirin, hydroxychloroquine (HCQ) and T-1105 seem to work better against CHIKV(Brazil) in glioblastoma cells than in Vero-B4. Finally, full genome analyses of the CHIKV isolates were done in order to determine their lineage and possibly explain differences in tissue range and antiviral compound efficacies. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11262-022-01892-x. Springer US 2022-03-26 2022 /pmc/articles/PMC8960095/ /pubmed/35347588 http://dx.doi.org/10.1007/s11262-022-01892-x Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Paper Hucke, Friederike I. L. Bestehorn-Willmann, Malena Bassetto, Marcella Brancale, Andrea Zanetta, Paola Bugert, Joachim J. CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251 |
title | CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251 |
title_full | CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251 |
title_fullStr | CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251 |
title_full_unstemmed | CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251 |
title_short | CHIKV strains Brazil (wt) and Ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show CPE in human glioblastoma cell lines U138 and U251 |
title_sort | chikv strains brazil (wt) and ross (lab-adapted) differ with regard to cell host range and antiviral sensitivity and show cpe in human glioblastoma cell lines u138 and u251 |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960095/ https://www.ncbi.nlm.nih.gov/pubmed/35347588 http://dx.doi.org/10.1007/s11262-022-01892-x |
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