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Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43

Human coronavirus OC43 (HCoV-OC43) is the coronavirus most associated with “common colds”, infections of the upper respiratory tract. Previously, we reported that direct interactions of nucleocapsid (N) protein and C-terminal domain of Spike protein (Spike CD) are essential for replication of SARS-C...

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Autores principales: Kim, Jinsoo, Kim, Minyoung, Kim, Dongbum, Park, Sangkyu, Kang, Mijeong, Baek, Kyeongbin, Choi, Jun-Kyu, Maharjan, Sony, Akauliya, Madhav, Lee, Younghee, Kwon, Hyung-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960273/
https://www.ncbi.nlm.nih.gov/pubmed/35359936
http://dx.doi.org/10.3389/fimmu.2022.835333
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author Kim, Jinsoo
Kim, Minyoung
Kim, Dongbum
Park, Sangkyu
Kang, Mijeong
Baek, Kyeongbin
Choi, Jun-Kyu
Maharjan, Sony
Akauliya, Madhav
Lee, Younghee
Kwon, Hyung-Joo
author_facet Kim, Jinsoo
Kim, Minyoung
Kim, Dongbum
Park, Sangkyu
Kang, Mijeong
Baek, Kyeongbin
Choi, Jun-Kyu
Maharjan, Sony
Akauliya, Madhav
Lee, Younghee
Kwon, Hyung-Joo
author_sort Kim, Jinsoo
collection PubMed
description Human coronavirus OC43 (HCoV-OC43) is the coronavirus most associated with “common colds”, infections of the upper respiratory tract. Previously, we reported that direct interactions of nucleocapsid (N) protein and C-terminal domain of Spike protein (Spike CD) are essential for replication of SARS-CoV-2 and MERS-CoV. Thus, we developed a novel ELISA-based strategy targeting these specific interactions to detect SARS-CoV-2 and MERS-CoV. Here, we investigated whether the same principles apply to HCoV-OC43. We discovered that the S protein of HCoV-OC43 interacts with N protein and that cell penetrating Spike CD peptide inhibits virus protein expression and replication of HCoV-OC43. The interaction between HCoV-OC43 S and N proteins were recapitulated with a recombinant HCoV-OC43 Spike CD fusion protein and a recombinant HCoV-OC43 N fusion protein in vitro. By producing an anti-HCoV-OC43 N protein-specific monoclonal antibody, we established a virus detection system based on the interaction between recombinant Spike CD and N protein of HCoV-OC43. We suggest that the interaction between Spike CD and N protein is conserved in coronaviruses and therefore could be a target for therapeutics against both novel coronavirus and its variants.
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spelling pubmed-89602732022-03-30 Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43 Kim, Jinsoo Kim, Minyoung Kim, Dongbum Park, Sangkyu Kang, Mijeong Baek, Kyeongbin Choi, Jun-Kyu Maharjan, Sony Akauliya, Madhav Lee, Younghee Kwon, Hyung-Joo Front Immunol Immunology Human coronavirus OC43 (HCoV-OC43) is the coronavirus most associated with “common colds”, infections of the upper respiratory tract. Previously, we reported that direct interactions of nucleocapsid (N) protein and C-terminal domain of Spike protein (Spike CD) are essential for replication of SARS-CoV-2 and MERS-CoV. Thus, we developed a novel ELISA-based strategy targeting these specific interactions to detect SARS-CoV-2 and MERS-CoV. Here, we investigated whether the same principles apply to HCoV-OC43. We discovered that the S protein of HCoV-OC43 interacts with N protein and that cell penetrating Spike CD peptide inhibits virus protein expression and replication of HCoV-OC43. The interaction between HCoV-OC43 S and N proteins were recapitulated with a recombinant HCoV-OC43 Spike CD fusion protein and a recombinant HCoV-OC43 N fusion protein in vitro. By producing an anti-HCoV-OC43 N protein-specific monoclonal antibody, we established a virus detection system based on the interaction between recombinant Spike CD and N protein of HCoV-OC43. We suggest that the interaction between Spike CD and N protein is conserved in coronaviruses and therefore could be a target for therapeutics against both novel coronavirus and its variants. Frontiers Media S.A. 2022-03-10 /pmc/articles/PMC8960273/ /pubmed/35359936 http://dx.doi.org/10.3389/fimmu.2022.835333 Text en Copyright © 2022 Kim, Kim, Kim, Park, Kang, Baek, Choi, Maharjan, Akauliya, Lee and Kwon https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Kim, Jinsoo
Kim, Minyoung
Kim, Dongbum
Park, Sangkyu
Kang, Mijeong
Baek, Kyeongbin
Choi, Jun-Kyu
Maharjan, Sony
Akauliya, Madhav
Lee, Younghee
Kwon, Hyung-Joo
Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43
title Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43
title_full Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43
title_fullStr Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43
title_full_unstemmed Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43
title_short Targeting the Interaction Between Spike Protein and Nucleocapsid Protein for Suppression and Detection of Human Coronavirus OC43
title_sort targeting the interaction between spike protein and nucleocapsid protein for suppression and detection of human coronavirus oc43
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960273/
https://www.ncbi.nlm.nih.gov/pubmed/35359936
http://dx.doi.org/10.3389/fimmu.2022.835333
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