Highly Expressed Soluble Recombinant Anti-GFP VHHs in Escherichia coli via Optimized Signal Peptides, Strains, and Inducers

Antigen-binding variable domains of the H chain of heavy-chain antibodies (VHHs), also known as nanobodies (Nbs), are of great interest in imaging technique, disease prevention, diagnosis, and therapy. High-level expression of soluble Nbs is very important for its industrial production. In this stud...

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Detalles Bibliográficos
Autores principales: Chao, Shuangying, Liu, Yuhang, Ding, Ning, Lin, Yue, Wang, Qian, Tan, Junwen, Li, Wei, Zheng, Yang, Hu, Xuejun, Li, Junming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960375/
https://www.ncbi.nlm.nih.gov/pubmed/35359590
http://dx.doi.org/10.3389/fmolb.2022.848829

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960375/
https://www.ncbi.nlm.nih.gov/pubmed/35359590
http://dx.doi.org/10.3389/fmolb.2022.848829

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