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Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects
Cytidine triphosphate synthase (CTPS) catalyzes the final step in pyrimidine de novo synthesis. In Arabidopsis, this protein family consists of five members (CTPS1–5), and all of them localize to the cytosol. Specifically, CTPS4 showed a massive upregulation of transcript levels during abiotic stres...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960734/ https://www.ncbi.nlm.nih.gov/pubmed/35360303 http://dx.doi.org/10.3389/fpls.2022.842156 |
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author | Krämer, Moritz Dörfer, Eva Hickl, Daniel Bellin, Leo Scherer, Vanessa Möhlmann, Torsten |
author_facet | Krämer, Moritz Dörfer, Eva Hickl, Daniel Bellin, Leo Scherer, Vanessa Möhlmann, Torsten |
author_sort | Krämer, Moritz |
collection | PubMed |
description | Cytidine triphosphate synthase (CTPS) catalyzes the final step in pyrimidine de novo synthesis. In Arabidopsis, this protein family consists of five members (CTPS1–5), and all of them localize to the cytosol. Specifically, CTPS4 showed a massive upregulation of transcript levels during abiotic stress, in line with increased staining of CTPS4 promoter:GUS lines in hypocotyl, root and to lesser extend leaf tissues. In a setup to study progressive drought stress, CTPS4 knockout mutants accumulated less fresh and dry weight at days 5–7 and showed impaired ability to recover from this stress after 3 days of rewatering. Surprisingly, a thorough physiological characterization of corresponding plants only revealed alterations in assimilation and accumulation of soluble sugars including those related to drought stress in the mutant. Bimolecular fluorescence complementation (BiFC) studies indicated the interaction of CTPS4 with other isoforms, possibly affecting cytoophidia (filaments formed by CTPS formation. Although the function of these structures has not been thoroughly investigated in plants, altered enzyme activity and effects on cell structure are reported in other organisms. CTPS activity is required for cell cycle progression and growth. Furthermore, drought can lead to the accumulation of reactive oxygen species (ROS) and by this, to DNA damage. We hypothesize that effects on the cell cycle or DNA repair might be relevant for the observed impaired reduced drought stress tolerance of CTPS4 mutants. |
format | Online Article Text |
id | pubmed-8960734 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89607342022-03-30 Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects Krämer, Moritz Dörfer, Eva Hickl, Daniel Bellin, Leo Scherer, Vanessa Möhlmann, Torsten Front Plant Sci Plant Science Cytidine triphosphate synthase (CTPS) catalyzes the final step in pyrimidine de novo synthesis. In Arabidopsis, this protein family consists of five members (CTPS1–5), and all of them localize to the cytosol. Specifically, CTPS4 showed a massive upregulation of transcript levels during abiotic stress, in line with increased staining of CTPS4 promoter:GUS lines in hypocotyl, root and to lesser extend leaf tissues. In a setup to study progressive drought stress, CTPS4 knockout mutants accumulated less fresh and dry weight at days 5–7 and showed impaired ability to recover from this stress after 3 days of rewatering. Surprisingly, a thorough physiological characterization of corresponding plants only revealed alterations in assimilation and accumulation of soluble sugars including those related to drought stress in the mutant. Bimolecular fluorescence complementation (BiFC) studies indicated the interaction of CTPS4 with other isoforms, possibly affecting cytoophidia (filaments formed by CTPS formation. Although the function of these structures has not been thoroughly investigated in plants, altered enzyme activity and effects on cell structure are reported in other organisms. CTPS activity is required for cell cycle progression and growth. Furthermore, drought can lead to the accumulation of reactive oxygen species (ROS) and by this, to DNA damage. We hypothesize that effects on the cell cycle or DNA repair might be relevant for the observed impaired reduced drought stress tolerance of CTPS4 mutants. Frontiers Media S.A. 2022-03-10 /pmc/articles/PMC8960734/ /pubmed/35360303 http://dx.doi.org/10.3389/fpls.2022.842156 Text en Copyright © 2022 Krämer, Dörfer, Hickl, Bellin, Scherer and Möhlmann. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Krämer, Moritz Dörfer, Eva Hickl, Daniel Bellin, Leo Scherer, Vanessa Möhlmann, Torsten Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects |
title | Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects |
title_full | Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects |
title_fullStr | Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects |
title_full_unstemmed | Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects |
title_short | Cytidine Triphosphate Synthase Four From Arabidopsis thaliana Attenuates Drought Stress Effects |
title_sort | cytidine triphosphate synthase four from arabidopsis thaliana attenuates drought stress effects |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960734/ https://www.ncbi.nlm.nih.gov/pubmed/35360303 http://dx.doi.org/10.3389/fpls.2022.842156 |
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