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MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis

Postmenopausal osteoporosis (PMOP) is the most common skeletal disease in postmenopausal women and has become a global public health issue. Emerging evidence demonstrated the important relationship between microRNAs and PMOP. However, miRNAs have not yet been reported in PMOP. Hence, the present stu...

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Autores principales: Shi, Hongli, Jiang, Xin, Xu, Cuidi, Cheng, Qun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960856/
https://www.ncbi.nlm.nih.gov/pubmed/35360081
http://dx.doi.org/10.3389/fendo.2022.819056
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author Shi, Hongli
Jiang, Xin
Xu, Cuidi
Cheng, Qun
author_facet Shi, Hongli
Jiang, Xin
Xu, Cuidi
Cheng, Qun
author_sort Shi, Hongli
collection PubMed
description Postmenopausal osteoporosis (PMOP) is the most common skeletal disease in postmenopausal women and has become a global public health issue. Emerging evidence demonstrated the important relationship between microRNAs and PMOP. However, miRNAs have not yet been reported in PMOP. Hence, the present study aimed to investigate the differences in miRNA expression profiles in PMOP with fragility fractures to identify the key circulating miRNAs in serum exosomes and to validate these molecules as potential biomarkers. Postmenopausal women with osteoporotic fracture and normal bone mass were enrolled. Serum exosomes were isolated by traditional differential ultracentrifugation from participants. Isolated exosomes were identified by electron microscopy, western blotting and nanoparticle-tracking analysis and then examined for exosomal small RNA sequencing. The expression of miRNAs was compared by sRNA deep sequencing and bioinformatics analysis. Three miRNAs (mir-324-3p, mir-766-3p and mir-1247-5p) were found to be associated with BMD of L1-L4, FN (femur neck) and TH (total hip), while mir-330-5p and mir-3124-5p were associated with BMD of FN and TH. Furthermore, mir-330-5p was found to promote the ALP activity of hBMSCs, while mir-3124-5p showed the opposite result. The results showed that serum exosomal miRNAs were differentially expressed in postmenopausal osteoporosis patients with fragility fractures. Our study provides the first evidence that exosomal miRNA profiling revealed aberrant circulating miRNA in postmenopausal osteoporosis. Mir-324-3p, mir-766-3p, mir-1247-5p, mir-330-5p and mir-3124-5p, which were associated with bone mineral density (BMD), may serve as candidate diagnostic biomarkers as well as potentially contribute to pathophysiology of PMOP.
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spelling pubmed-89608562022-03-30 MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis Shi, Hongli Jiang, Xin Xu, Cuidi Cheng, Qun Front Endocrinol (Lausanne) Endocrinology Postmenopausal osteoporosis (PMOP) is the most common skeletal disease in postmenopausal women and has become a global public health issue. Emerging evidence demonstrated the important relationship between microRNAs and PMOP. However, miRNAs have not yet been reported in PMOP. Hence, the present study aimed to investigate the differences in miRNA expression profiles in PMOP with fragility fractures to identify the key circulating miRNAs in serum exosomes and to validate these molecules as potential biomarkers. Postmenopausal women with osteoporotic fracture and normal bone mass were enrolled. Serum exosomes were isolated by traditional differential ultracentrifugation from participants. Isolated exosomes were identified by electron microscopy, western blotting and nanoparticle-tracking analysis and then examined for exosomal small RNA sequencing. The expression of miRNAs was compared by sRNA deep sequencing and bioinformatics analysis. Three miRNAs (mir-324-3p, mir-766-3p and mir-1247-5p) were found to be associated with BMD of L1-L4, FN (femur neck) and TH (total hip), while mir-330-5p and mir-3124-5p were associated with BMD of FN and TH. Furthermore, mir-330-5p was found to promote the ALP activity of hBMSCs, while mir-3124-5p showed the opposite result. The results showed that serum exosomal miRNAs were differentially expressed in postmenopausal osteoporosis patients with fragility fractures. Our study provides the first evidence that exosomal miRNA profiling revealed aberrant circulating miRNA in postmenopausal osteoporosis. Mir-324-3p, mir-766-3p, mir-1247-5p, mir-330-5p and mir-3124-5p, which were associated with bone mineral density (BMD), may serve as candidate diagnostic biomarkers as well as potentially contribute to pathophysiology of PMOP. Frontiers Media S.A. 2022-03-10 /pmc/articles/PMC8960856/ /pubmed/35360081 http://dx.doi.org/10.3389/fendo.2022.819056 Text en Copyright © 2022 Shi, Jiang, Xu and Cheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Shi, Hongli
Jiang, Xin
Xu, Cuidi
Cheng, Qun
MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis
title MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis
title_full MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis
title_fullStr MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis
title_full_unstemmed MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis
title_short MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis
title_sort micrornas in serum exosomes as circulating biomarkers for postmenopausal osteoporosis
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960856/
https://www.ncbi.nlm.nih.gov/pubmed/35360081
http://dx.doi.org/10.3389/fendo.2022.819056
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