Cargando…
MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis
Postmenopausal osteoporosis (PMOP) is the most common skeletal disease in postmenopausal women and has become a global public health issue. Emerging evidence demonstrated the important relationship between microRNAs and PMOP. However, miRNAs have not yet been reported in PMOP. Hence, the present stu...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960856/ https://www.ncbi.nlm.nih.gov/pubmed/35360081 http://dx.doi.org/10.3389/fendo.2022.819056 |
_version_ | 1784677470241292288 |
---|---|
author | Shi, Hongli Jiang, Xin Xu, Cuidi Cheng, Qun |
author_facet | Shi, Hongli Jiang, Xin Xu, Cuidi Cheng, Qun |
author_sort | Shi, Hongli |
collection | PubMed |
description | Postmenopausal osteoporosis (PMOP) is the most common skeletal disease in postmenopausal women and has become a global public health issue. Emerging evidence demonstrated the important relationship between microRNAs and PMOP. However, miRNAs have not yet been reported in PMOP. Hence, the present study aimed to investigate the differences in miRNA expression profiles in PMOP with fragility fractures to identify the key circulating miRNAs in serum exosomes and to validate these molecules as potential biomarkers. Postmenopausal women with osteoporotic fracture and normal bone mass were enrolled. Serum exosomes were isolated by traditional differential ultracentrifugation from participants. Isolated exosomes were identified by electron microscopy, western blotting and nanoparticle-tracking analysis and then examined for exosomal small RNA sequencing. The expression of miRNAs was compared by sRNA deep sequencing and bioinformatics analysis. Three miRNAs (mir-324-3p, mir-766-3p and mir-1247-5p) were found to be associated with BMD of L1-L4, FN (femur neck) and TH (total hip), while mir-330-5p and mir-3124-5p were associated with BMD of FN and TH. Furthermore, mir-330-5p was found to promote the ALP activity of hBMSCs, while mir-3124-5p showed the opposite result. The results showed that serum exosomal miRNAs were differentially expressed in postmenopausal osteoporosis patients with fragility fractures. Our study provides the first evidence that exosomal miRNA profiling revealed aberrant circulating miRNA in postmenopausal osteoporosis. Mir-324-3p, mir-766-3p, mir-1247-5p, mir-330-5p and mir-3124-5p, which were associated with bone mineral density (BMD), may serve as candidate diagnostic biomarkers as well as potentially contribute to pathophysiology of PMOP. |
format | Online Article Text |
id | pubmed-8960856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89608562022-03-30 MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis Shi, Hongli Jiang, Xin Xu, Cuidi Cheng, Qun Front Endocrinol (Lausanne) Endocrinology Postmenopausal osteoporosis (PMOP) is the most common skeletal disease in postmenopausal women and has become a global public health issue. Emerging evidence demonstrated the important relationship between microRNAs and PMOP. However, miRNAs have not yet been reported in PMOP. Hence, the present study aimed to investigate the differences in miRNA expression profiles in PMOP with fragility fractures to identify the key circulating miRNAs in serum exosomes and to validate these molecules as potential biomarkers. Postmenopausal women with osteoporotic fracture and normal bone mass were enrolled. Serum exosomes were isolated by traditional differential ultracentrifugation from participants. Isolated exosomes were identified by electron microscopy, western blotting and nanoparticle-tracking analysis and then examined for exosomal small RNA sequencing. The expression of miRNAs was compared by sRNA deep sequencing and bioinformatics analysis. Three miRNAs (mir-324-3p, mir-766-3p and mir-1247-5p) were found to be associated with BMD of L1-L4, FN (femur neck) and TH (total hip), while mir-330-5p and mir-3124-5p were associated with BMD of FN and TH. Furthermore, mir-330-5p was found to promote the ALP activity of hBMSCs, while mir-3124-5p showed the opposite result. The results showed that serum exosomal miRNAs were differentially expressed in postmenopausal osteoporosis patients with fragility fractures. Our study provides the first evidence that exosomal miRNA profiling revealed aberrant circulating miRNA in postmenopausal osteoporosis. Mir-324-3p, mir-766-3p, mir-1247-5p, mir-330-5p and mir-3124-5p, which were associated with bone mineral density (BMD), may serve as candidate diagnostic biomarkers as well as potentially contribute to pathophysiology of PMOP. Frontiers Media S.A. 2022-03-10 /pmc/articles/PMC8960856/ /pubmed/35360081 http://dx.doi.org/10.3389/fendo.2022.819056 Text en Copyright © 2022 Shi, Jiang, Xu and Cheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Shi, Hongli Jiang, Xin Xu, Cuidi Cheng, Qun MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis |
title | MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis |
title_full | MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis |
title_fullStr | MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis |
title_full_unstemmed | MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis |
title_short | MicroRNAs in Serum Exosomes as Circulating Biomarkers for Postmenopausal Osteoporosis |
title_sort | micrornas in serum exosomes as circulating biomarkers for postmenopausal osteoporosis |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960856/ https://www.ncbi.nlm.nih.gov/pubmed/35360081 http://dx.doi.org/10.3389/fendo.2022.819056 |
work_keys_str_mv | AT shihongli micrornasinserumexosomesascirculatingbiomarkersforpostmenopausalosteoporosis AT jiangxin micrornasinserumexosomesascirculatingbiomarkersforpostmenopausalosteoporosis AT xucuidi micrornasinserumexosomesascirculatingbiomarkersforpostmenopausalosteoporosis AT chengqun micrornasinserumexosomesascirculatingbiomarkersforpostmenopausalosteoporosis |