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Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts

Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethan...

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Autores principales: Rodrigues, Isabella C. P., Lopes, Éder S. N., Pereira, Karina D., Huber, Stephany C., Jardini, André Luiz, Annichino-Bizzacchi, Joyce M., Luchessi, Augusto D., Gabriel, Laís P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960935/
https://www.ncbi.nlm.nih.gov/pubmed/35347181
http://dx.doi.org/10.1038/s41598-022-09040-z
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author Rodrigues, Isabella C. P.
Lopes, Éder S. N.
Pereira, Karina D.
Huber, Stephany C.
Jardini, André Luiz
Annichino-Bizzacchi, Joyce M.
Luchessi, Augusto D.
Gabriel, Laís P.
author_facet Rodrigues, Isabella C. P.
Lopes, Éder S. N.
Pereira, Karina D.
Huber, Stephany C.
Jardini, André Luiz
Annichino-Bizzacchi, Joyce M.
Luchessi, Augusto D.
Gabriel, Laís P.
author_sort Rodrigues, Isabella C. P.
collection PubMed
description Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethane (PU) was blended with ECM proteins collagen and elastin (Col-El) and gelatin (Gel) to produce fibrous scaffolds by using the rotary jet spinning (RJS) technique, and their effects on in vitro properties were evaluated. Morphological and structural characterization of the scaffolds was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Micrometric fibers with nanometric rugosity were obtained. Col-El and Gel reduced the mechanical strength and increased the hydrophilicity and degradation rates of PU. No platelet adhesion or activation was observed. The addition of proteins to the PU blend increased the viability, adhesion, and proliferation of human umbilical vein endothelial cells (HUVECs). Therefore, PU-Col-El and PU-Gel scaffolds are promising biomaterials for vascular graft applications.
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spelling pubmed-89609352022-03-30 Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts Rodrigues, Isabella C. P. Lopes, Éder S. N. Pereira, Karina D. Huber, Stephany C. Jardini, André Luiz Annichino-Bizzacchi, Joyce M. Luchessi, Augusto D. Gabriel, Laís P. Sci Rep Article Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethane (PU) was blended with ECM proteins collagen and elastin (Col-El) and gelatin (Gel) to produce fibrous scaffolds by using the rotary jet spinning (RJS) technique, and their effects on in vitro properties were evaluated. Morphological and structural characterization of the scaffolds was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Micrometric fibers with nanometric rugosity were obtained. Col-El and Gel reduced the mechanical strength and increased the hydrophilicity and degradation rates of PU. No platelet adhesion or activation was observed. The addition of proteins to the PU blend increased the viability, adhesion, and proliferation of human umbilical vein endothelial cells (HUVECs). Therefore, PU-Col-El and PU-Gel scaffolds are promising biomaterials for vascular graft applications. Nature Publishing Group UK 2022-03-28 /pmc/articles/PMC8960935/ /pubmed/35347181 http://dx.doi.org/10.1038/s41598-022-09040-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Rodrigues, Isabella C. P.
Lopes, Éder S. N.
Pereira, Karina D.
Huber, Stephany C.
Jardini, André Luiz
Annichino-Bizzacchi, Joyce M.
Luchessi, Augusto D.
Gabriel, Laís P.
Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
title Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
title_full Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
title_fullStr Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
title_full_unstemmed Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
title_short Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
title_sort extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960935/
https://www.ncbi.nlm.nih.gov/pubmed/35347181
http://dx.doi.org/10.1038/s41598-022-09040-z
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