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Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens

This study aimed to investigate the effects of in ovo feeding (IOF) and dietary addition (DA) oils on growth, development and immune function of broiler chickens. In experiment 1, a total of 500 eggs were randomly assigned to 3 treatments: non-injected group (CON) with 100 eggs; soybean oil injected...

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Autores principales: Ma, Haiyan, Liang, Saisai, Wu, Hanyue, Du, Caiyun, Ren, Zhouzheng, Yang, Xiaojun, Yang, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960950/
https://www.ncbi.nlm.nih.gov/pubmed/35339935
http://dx.doi.org/10.1016/j.psj.2022.101815
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author Ma, Haiyan
Liang, Saisai
Wu, Hanyue
Du, Caiyun
Ren, Zhouzheng
Yang, Xiaojun
Yang, Xin
author_facet Ma, Haiyan
Liang, Saisai
Wu, Hanyue
Du, Caiyun
Ren, Zhouzheng
Yang, Xiaojun
Yang, Xin
author_sort Ma, Haiyan
collection PubMed
description This study aimed to investigate the effects of in ovo feeding (IOF) and dietary addition (DA) oils on growth, development and immune function of broiler chickens. In experiment 1, a total of 500 eggs were randomly assigned to 3 treatments: non-injected group (CON) with 100 eggs; soybean oil injected group (SO) with 200 eggs and linseed oil injected group (LO) with 200 eggs. Results showed that there were no detrimental effects of IOF of oils on embryonic development. In experiment 2, a two factor experimental design was adopted. After hatching, 120 chicks which came from each oil-injected group were divided into 2 treatments with 6 replicates, and chickens were fed soybean oil diet and linseed oil diet, respectively. The results showed that DA linseed oil increased final body weight (FBW) of broilers at d 21 post hatch, IOF of linseed oil decreased average daily feed intake (ADFI) and feed conversion ratio (FCR) of broilers from d 1 to 21 (P < 0.05), while the plasma leptin level of 21-day-old broilers was increased by IOF or DA linseed oil (P < 0.05). Main effect analysis showed that DA linseed oil increased the spleen index and mRNA expression of IFN-γ in spleen of broilers at 7 d of age (P < 0.05). IOF of linseed oil upregulated the mRNA expression of IFN-γ in the spleen of chicks at 1 d and mRNA expression of IL-2 and IL-4 in spleen of broilers at 21 d (P < 0.05), and the interaction effect showed that IOF and DA linseed oil synergically increased the expression of IL-2 and IL-4 in spleen of broilers at 21 d. Compared with SO group, LO increased the Shannon index of hatching-day cecum microflora (P < 0.05). Principal co-ordinates analysis (PcoA) showed that LO group clearly separated from CON and SO groups. Finally, Spearman correlation analysis also manifested that Alkalicoccus was significantly correlated with spleen index and mRNA expression of IL-2, and Phreatobacter was significantly correlated with the mRNA expression of IL-2 and IFN-γ in spleen, Acinetobacter had a positive correlation with thymus index (P < 0.05). In conclusion, IOF of linseed oil reduced the ADFI and FCR of broilers and increased the species diversity and changed the structure of cecal microflora of chicken embryos at the 19th day of incubation (E19). Immune function of broilers spleen was also regulated by IOF and DA linseed oil.
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spelling pubmed-89609502022-03-30 Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens Ma, Haiyan Liang, Saisai Wu, Hanyue Du, Caiyun Ren, Zhouzheng Yang, Xiaojun Yang, Xin Poult Sci METABOLISM AND NUTRITION This study aimed to investigate the effects of in ovo feeding (IOF) and dietary addition (DA) oils on growth, development and immune function of broiler chickens. In experiment 1, a total of 500 eggs were randomly assigned to 3 treatments: non-injected group (CON) with 100 eggs; soybean oil injected group (SO) with 200 eggs and linseed oil injected group (LO) with 200 eggs. Results showed that there were no detrimental effects of IOF of oils on embryonic development. In experiment 2, a two factor experimental design was adopted. After hatching, 120 chicks which came from each oil-injected group were divided into 2 treatments with 6 replicates, and chickens were fed soybean oil diet and linseed oil diet, respectively. The results showed that DA linseed oil increased final body weight (FBW) of broilers at d 21 post hatch, IOF of linseed oil decreased average daily feed intake (ADFI) and feed conversion ratio (FCR) of broilers from d 1 to 21 (P < 0.05), while the plasma leptin level of 21-day-old broilers was increased by IOF or DA linseed oil (P < 0.05). Main effect analysis showed that DA linseed oil increased the spleen index and mRNA expression of IFN-γ in spleen of broilers at 7 d of age (P < 0.05). IOF of linseed oil upregulated the mRNA expression of IFN-γ in the spleen of chicks at 1 d and mRNA expression of IL-2 and IL-4 in spleen of broilers at 21 d (P < 0.05), and the interaction effect showed that IOF and DA linseed oil synergically increased the expression of IL-2 and IL-4 in spleen of broilers at 21 d. Compared with SO group, LO increased the Shannon index of hatching-day cecum microflora (P < 0.05). Principal co-ordinates analysis (PcoA) showed that LO group clearly separated from CON and SO groups. Finally, Spearman correlation analysis also manifested that Alkalicoccus was significantly correlated with spleen index and mRNA expression of IL-2, and Phreatobacter was significantly correlated with the mRNA expression of IL-2 and IFN-γ in spleen, Acinetobacter had a positive correlation with thymus index (P < 0.05). In conclusion, IOF of linseed oil reduced the ADFI and FCR of broilers and increased the species diversity and changed the structure of cecal microflora of chicken embryos at the 19th day of incubation (E19). Immune function of broilers spleen was also regulated by IOF and DA linseed oil. Elsevier 2022-02-25 /pmc/articles/PMC8960950/ /pubmed/35339935 http://dx.doi.org/10.1016/j.psj.2022.101815 Text en © 2022 Published by Elsevier Inc. on behalf of Poultry Science Association Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle METABOLISM AND NUTRITION
Ma, Haiyan
Liang, Saisai
Wu, Hanyue
Du, Caiyun
Ren, Zhouzheng
Yang, Xiaojun
Yang, Xin
Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens
title Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens
title_full Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens
title_fullStr Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens
title_full_unstemmed Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens
title_short Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens
title_sort effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens
topic METABOLISM AND NUTRITION
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960950/
https://www.ncbi.nlm.nih.gov/pubmed/35339935
http://dx.doi.org/10.1016/j.psj.2022.101815
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