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In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration
The culling of day-old male chicks is an animal welfare issue within the laying hen industry that raises substantial ethical concern. Alternative methods are sought to pre-select males during embryonic development. This method is called in ovo sexing and allows more humane male culling. A robust and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960969/ https://www.ncbi.nlm.nih.gov/pubmed/35339934 http://dx.doi.org/10.1016/j.psj.2022.101782 |
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author | Corion, Matthias Keresztes, Janos De Ketelaere, Bart Saeys, Wouters |
author_facet | Corion, Matthias Keresztes, Janos De Ketelaere, Bart Saeys, Wouters |
author_sort | Corion, Matthias |
collection | PubMed |
description | The culling of day-old male chicks is an animal welfare issue within the laying hen industry that raises substantial ethical concern. Alternative methods are sought to pre-select males during embryonic development. This method is called in ovo sexing and allows more humane male culling. A robust and non-invasive in ovo color sexing technique was developed and validated in this research. To this end, visible-near-infrared (vis-NIR) point spectroscopy was used, which has advantages over state-of-the-art hyperspectral imaging in terms of accuracy and cost. Two independent experiments were each conducted on a batch of 600 Isa Brown eggs. These eggs were individually illuminated on d 8 to 14, and d 18 of incubation by a halogen lamp and the signal was measured in the vis-NIR range from 300 to 1,145 nm. Next, optimal preprocessing strategies were applied and partial least squares discriminant analysis (PLS-DA) models were built and further optimized after performing a forward interval partial least squares (FiPLS) for variable selection. Results demonstrated that d 12 is too early for vis-NIR in ovo sexing, resulting in a prediction accuracy of 86.49%. However, after 13 d of incubation, an accuracy of 97.78% was obtained, increasing to 99.52% on d 14. Furthermore, these accuracies were higher than earlier reported percentages from hyperspectral imaging and successful sexing was expedited from d 14 to d 13. Moreover, prediction improvement up to 99.05% was obtained on d 13 by correcting for the variability in eggshell properties using d 0 eggshell corrections. Applying the method on d 18 resulted in a lower accuracy of 94.62% due to stronger light attenuation by the growing embryos. Finally, a reduced spectral range of 749 to 861 nm was found to be sufficient for correct classification of 98.46% of the eggs. This paves the way for high-throughput and cost-efficient usage of smaller and cheaper spectrophotometers in commercial hatcheries. |
format | Online Article Text |
id | pubmed-8960969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-89609692022-03-30 In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration Corion, Matthias Keresztes, Janos De Ketelaere, Bart Saeys, Wouters Poult Sci MANAGEMENT AND PRODUCTION The culling of day-old male chicks is an animal welfare issue within the laying hen industry that raises substantial ethical concern. Alternative methods are sought to pre-select males during embryonic development. This method is called in ovo sexing and allows more humane male culling. A robust and non-invasive in ovo color sexing technique was developed and validated in this research. To this end, visible-near-infrared (vis-NIR) point spectroscopy was used, which has advantages over state-of-the-art hyperspectral imaging in terms of accuracy and cost. Two independent experiments were each conducted on a batch of 600 Isa Brown eggs. These eggs were individually illuminated on d 8 to 14, and d 18 of incubation by a halogen lamp and the signal was measured in the vis-NIR range from 300 to 1,145 nm. Next, optimal preprocessing strategies were applied and partial least squares discriminant analysis (PLS-DA) models were built and further optimized after performing a forward interval partial least squares (FiPLS) for variable selection. Results demonstrated that d 12 is too early for vis-NIR in ovo sexing, resulting in a prediction accuracy of 86.49%. However, after 13 d of incubation, an accuracy of 97.78% was obtained, increasing to 99.52% on d 14. Furthermore, these accuracies were higher than earlier reported percentages from hyperspectral imaging and successful sexing was expedited from d 14 to d 13. Moreover, prediction improvement up to 99.05% was obtained on d 13 by correcting for the variability in eggshell properties using d 0 eggshell corrections. Applying the method on d 18 resulted in a lower accuracy of 94.62% due to stronger light attenuation by the growing embryos. Finally, a reduced spectral range of 749 to 861 nm was found to be sufficient for correct classification of 98.46% of the eggs. This paves the way for high-throughput and cost-efficient usage of smaller and cheaper spectrophotometers in commercial hatcheries. Elsevier 2022-02-16 /pmc/articles/PMC8960969/ /pubmed/35339934 http://dx.doi.org/10.1016/j.psj.2022.101782 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | MANAGEMENT AND PRODUCTION Corion, Matthias Keresztes, Janos De Ketelaere, Bart Saeys, Wouters In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration |
title | In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration |
title_full | In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration |
title_fullStr | In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration |
title_full_unstemmed | In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration |
title_short | In ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration |
title_sort | in ovo sexing of eggs from brown breeds with a gender-specific color using visible-near-infrared spectroscopy: effect of incubation day and measurement configuration |
topic | MANAGEMENT AND PRODUCTION |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8960969/ https://www.ncbi.nlm.nih.gov/pubmed/35339934 http://dx.doi.org/10.1016/j.psj.2022.101782 |
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