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A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots

Metaxylem vessels in maize brace roots are key tissue, and their number (MVN) affects plant water and inorganic salt transportation and lodging resistance. Dissecting the genetic basis of MVN in maize brace roots can help guide the genetic improvement of maize drought resistance and lodging resistan...

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Autores principales: Liu, Meiling, Zhang, Meng, Yu, Shuai, Li, Xiaoyang, Zhang, Ao, Cui, Zhenhai, Dong, Xiaomei, Fan, Jinjuan, Zhang, Lijun, Li, Cong, Ruan, Yanye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961028/
https://www.ncbi.nlm.nih.gov/pubmed/35360304
http://dx.doi.org/10.3389/fpls.2022.847234
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author Liu, Meiling
Zhang, Meng
Yu, Shuai
Li, Xiaoyang
Zhang, Ao
Cui, Zhenhai
Dong, Xiaomei
Fan, Jinjuan
Zhang, Lijun
Li, Cong
Ruan, Yanye
author_facet Liu, Meiling
Zhang, Meng
Yu, Shuai
Li, Xiaoyang
Zhang, Ao
Cui, Zhenhai
Dong, Xiaomei
Fan, Jinjuan
Zhang, Lijun
Li, Cong
Ruan, Yanye
author_sort Liu, Meiling
collection PubMed
description Metaxylem vessels in maize brace roots are key tissue, and their number (MVN) affects plant water and inorganic salt transportation and lodging resistance. Dissecting the genetic basis of MVN in maize brace roots can help guide the genetic improvement of maize drought resistance and lodging resistance during late developmental stages. In this study, we used 508 inbred lines with tropical, subtropical, and temperate backgrounds to analyze the genetic architecture of MVN in maize brace roots. The phenotypic variation in MVN in brace roots was evaluated in three environments, which revealed broad natural variation and relative low levels of heritability (h(2) = 0.42). Stiff-stalk lines with a temperate background tended to have higher MVNs than plants in other genetic backgrounds. MVN was significantly positively correlated with plant height, tassel maximum axis length, ear length, and kernel number per row, which indicates that MVN may affect plant morphological development and yield. In addition, MVN was extremely significantly negatively correlated with brace root radius, but significantly positively correlated with brace root angle (BRA), diameter, and number, thus suggesting that the morphological function of some brace root traits may be essentially determined by MVN. Association analysis of MVN in brace roots combined 1,253,814 single nucleotide polymorphisms (SNPs) using FarmCPU revealed a total of nine SNPs significantly associated with MVN at P < 7.96 × 10(–7). Five candidate genes for MVN that may participate in secondary wall formation (GRMZM2G168365, GRMZM2G470499, and GRMZM2G028982) and regulate flowering time (GRMZM2G381691 and GRMZM2G449165). These results provide useful information for understanding the genetic basis of MVN in brace root development. Further functional studies of identified candidate genes should help elucidate the molecular pathways that regulate MVN in maize brace roots.
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spelling pubmed-89610282022-03-30 A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots Liu, Meiling Zhang, Meng Yu, Shuai Li, Xiaoyang Zhang, Ao Cui, Zhenhai Dong, Xiaomei Fan, Jinjuan Zhang, Lijun Li, Cong Ruan, Yanye Front Plant Sci Plant Science Metaxylem vessels in maize brace roots are key tissue, and their number (MVN) affects plant water and inorganic salt transportation and lodging resistance. Dissecting the genetic basis of MVN in maize brace roots can help guide the genetic improvement of maize drought resistance and lodging resistance during late developmental stages. In this study, we used 508 inbred lines with tropical, subtropical, and temperate backgrounds to analyze the genetic architecture of MVN in maize brace roots. The phenotypic variation in MVN in brace roots was evaluated in three environments, which revealed broad natural variation and relative low levels of heritability (h(2) = 0.42). Stiff-stalk lines with a temperate background tended to have higher MVNs than plants in other genetic backgrounds. MVN was significantly positively correlated with plant height, tassel maximum axis length, ear length, and kernel number per row, which indicates that MVN may affect plant morphological development and yield. In addition, MVN was extremely significantly negatively correlated with brace root radius, but significantly positively correlated with brace root angle (BRA), diameter, and number, thus suggesting that the morphological function of some brace root traits may be essentially determined by MVN. Association analysis of MVN in brace roots combined 1,253,814 single nucleotide polymorphisms (SNPs) using FarmCPU revealed a total of nine SNPs significantly associated with MVN at P < 7.96 × 10(–7). Five candidate genes for MVN that may participate in secondary wall formation (GRMZM2G168365, GRMZM2G470499, and GRMZM2G028982) and regulate flowering time (GRMZM2G381691 and GRMZM2G449165). These results provide useful information for understanding the genetic basis of MVN in brace root development. Further functional studies of identified candidate genes should help elucidate the molecular pathways that regulate MVN in maize brace roots. Frontiers Media S.A. 2022-03-10 /pmc/articles/PMC8961028/ /pubmed/35360304 http://dx.doi.org/10.3389/fpls.2022.847234 Text en Copyright © 2022 Liu, Zhang, Yu, Li, Zhang, Cui, Dong, Fan, Zhang, Li and Ruan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Meiling
Zhang, Meng
Yu, Shuai
Li, Xiaoyang
Zhang, Ao
Cui, Zhenhai
Dong, Xiaomei
Fan, Jinjuan
Zhang, Lijun
Li, Cong
Ruan, Yanye
A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots
title A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots
title_full A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots
title_fullStr A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots
title_full_unstemmed A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots
title_short A Genome-Wide Association Study Dissects the Genetic Architecture of the Metaxylem Vessel Number in Maize Brace Roots
title_sort genome-wide association study dissects the genetic architecture of the metaxylem vessel number in maize brace roots
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961028/
https://www.ncbi.nlm.nih.gov/pubmed/35360304
http://dx.doi.org/10.3389/fpls.2022.847234
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