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Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda
Brewers’ spent grain (BSG) is a low‐value by‐product of the brewing process, which is produced in large quantities every year. In this study, the lignocellulosic feedstock (solid BSG) was used to optimize fermentations with Cellulomonas uda. Under aerobic conditions, maximum cellulase activities of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961044/ https://www.ncbi.nlm.nih.gov/pubmed/35382540 http://dx.doi.org/10.1002/elsc.202100053 |
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author | Akermann, Alexander Weiermüller, Jens Chodorski, Jonas Nicolai Nestriepke, Malte Jakob Baclig, Maria Teresa Ulber, Roland |
author_facet | Akermann, Alexander Weiermüller, Jens Chodorski, Jonas Nicolai Nestriepke, Malte Jakob Baclig, Maria Teresa Ulber, Roland |
author_sort | Akermann, Alexander |
collection | PubMed |
description | Brewers’ spent grain (BSG) is a low‐value by‐product of the brewing process, which is produced in large quantities every year. In this study, the lignocellulosic feedstock (solid BSG) was used to optimize fermentations with Cellulomonas uda. Under aerobic conditions, maximum cellulase activities of 0.98 nkat∙mL(−1), maximum xylanase activities of 5.00 nkat∙mL(−1) and cell yields of 0.22 g(Cells)∙g(BSG) (−1) were achieved. Under anaerobic conditions, enzyme activities and cell yields were lower, but valuable liquid products (organic acids, ethanol) were produced with a yield of 0.41 g(Prod)∙g(BSG) (−1). The growth phase of the organisms was monitored by measuring extracellular concentrations of two fluorophores pyridoxin (aerobic) and tryptophan (anaerobic) and by cell count. By combining reductive with anaerobic conditions, the ratio of ethanol to acetate was increased from 1.08 to 1.59 mol(EtOH)∙mol(Ac) (−1). This ratio was further improved to 9.2 mol(EtOH)∙mol(Ac) (−1) by lowering the pH from 7.4 to 5.0 without decreasing the final ethanol concentration. A fermentation in a bioreactor with 15 w% BSG instead of 5 w% BSG quadrupled the acetate concentration, whilst ethanol was removed by gas stripping. This study provides various ideas for optimizing and monitoring fermentations with solid substrates, which can support feasibility and incorporation into holistic biorefining approaches in the future. |
format | Online Article Text |
id | pubmed-8961044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89610442022-04-04 Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda Akermann, Alexander Weiermüller, Jens Chodorski, Jonas Nicolai Nestriepke, Malte Jakob Baclig, Maria Teresa Ulber, Roland Eng Life Sci Research Articles Brewers’ spent grain (BSG) is a low‐value by‐product of the brewing process, which is produced in large quantities every year. In this study, the lignocellulosic feedstock (solid BSG) was used to optimize fermentations with Cellulomonas uda. Under aerobic conditions, maximum cellulase activities of 0.98 nkat∙mL(−1), maximum xylanase activities of 5.00 nkat∙mL(−1) and cell yields of 0.22 g(Cells)∙g(BSG) (−1) were achieved. Under anaerobic conditions, enzyme activities and cell yields were lower, but valuable liquid products (organic acids, ethanol) were produced with a yield of 0.41 g(Prod)∙g(BSG) (−1). The growth phase of the organisms was monitored by measuring extracellular concentrations of two fluorophores pyridoxin (aerobic) and tryptophan (anaerobic) and by cell count. By combining reductive with anaerobic conditions, the ratio of ethanol to acetate was increased from 1.08 to 1.59 mol(EtOH)∙mol(Ac) (−1). This ratio was further improved to 9.2 mol(EtOH)∙mol(Ac) (−1) by lowering the pH from 7.4 to 5.0 without decreasing the final ethanol concentration. A fermentation in a bioreactor with 15 w% BSG instead of 5 w% BSG quadrupled the acetate concentration, whilst ethanol was removed by gas stripping. This study provides various ideas for optimizing and monitoring fermentations with solid substrates, which can support feasibility and incorporation into holistic biorefining approaches in the future. John Wiley and Sons Inc. 2021-08-27 /pmc/articles/PMC8961044/ /pubmed/35382540 http://dx.doi.org/10.1002/elsc.202100053 Text en © 2021 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Akermann, Alexander Weiermüller, Jens Chodorski, Jonas Nicolai Nestriepke, Malte Jakob Baclig, Maria Teresa Ulber, Roland Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda |
title | Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda
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title_full | Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda
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title_fullStr | Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda
|
title_full_unstemmed | Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda
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title_short | Optimization of bioprocesses with Brewers’ spent grain and Cellulomonas uda
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title_sort | optimization of bioprocesses with brewers’ spent grain and cellulomonas uda |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961044/ https://www.ncbi.nlm.nih.gov/pubmed/35382540 http://dx.doi.org/10.1002/elsc.202100053 |
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