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Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate

The conversion of carbohydrates in biomass via fermentation is an important component of an overall strategy to decarbonize the production of fuels and chemicals. Owing to the cost and resources required to produce biomass hydrolysates, the economic and environmental sustainability of these fermenta...

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Autores principales: Huang, Xing‐Feng, Reardon, Kenneth F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961051/
https://www.ncbi.nlm.nih.gov/pubmed/35382533
http://dx.doi.org/10.1002/elsc.202100095
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author Huang, Xing‐Feng
Reardon, Kenneth F.
author_facet Huang, Xing‐Feng
Reardon, Kenneth F.
author_sort Huang, Xing‐Feng
collection PubMed
description The conversion of carbohydrates in biomass via fermentation is an important component of an overall strategy to decarbonize the production of fuels and chemicals. Owing to the cost and resources required to produce biomass hydrolysates, the economic and environmental sustainability of these fermentation processes requires that they operate with high yields, sugar conversion, and productivity. Immobilized‐cell technology in a continuous bioprocess can achieve significantly higher volumetric productivities than is possible from standard batch fermentation using free cells. Here, we demonstrate approaches for improvement of ethanol yield from algal hydrolysates and a mock hydrolysate medium. Saccharomyces cerevisiae was immobilized in alginate and incorporated into a two‐column immobilized cell reactor system. Furthermore, the yeast quorum‐sensing molecule, 2‐phenylethanol, was added to improve ethanol yield by restricting growth and diverting sugar to ethanol. The bioreactor system could achieve high ethanol volumetric productivity (>20 g/L(reactor)·h) and high glucose conversion (>99%) in mock hydrolysate, while the addition of 0.2% 2‐phenylethanol resulted in 4.9% higher ethanol yield. With an algal hydrolysate of <10 g/L sugar, the ethanol volumetric productivity reached 9.8 g/L(reactor)·h, and the addition of 0.2% 2‐phenylethanol increased the ethanol yield by up to 7.4%. These results demonstrate the feasibility of novel strategies to achieve sustainability goals in biomass conversions.
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spelling pubmed-89610512022-04-04 Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate Huang, Xing‐Feng Reardon, Kenneth F. Eng Life Sci Research Articles The conversion of carbohydrates in biomass via fermentation is an important component of an overall strategy to decarbonize the production of fuels and chemicals. Owing to the cost and resources required to produce biomass hydrolysates, the economic and environmental sustainability of these fermentation processes requires that they operate with high yields, sugar conversion, and productivity. Immobilized‐cell technology in a continuous bioprocess can achieve significantly higher volumetric productivities than is possible from standard batch fermentation using free cells. Here, we demonstrate approaches for improvement of ethanol yield from algal hydrolysates and a mock hydrolysate medium. Saccharomyces cerevisiae was immobilized in alginate and incorporated into a two‐column immobilized cell reactor system. Furthermore, the yeast quorum‐sensing molecule, 2‐phenylethanol, was added to improve ethanol yield by restricting growth and diverting sugar to ethanol. The bioreactor system could achieve high ethanol volumetric productivity (>20 g/L(reactor)·h) and high glucose conversion (>99%) in mock hydrolysate, while the addition of 0.2% 2‐phenylethanol resulted in 4.9% higher ethanol yield. With an algal hydrolysate of <10 g/L sugar, the ethanol volumetric productivity reached 9.8 g/L(reactor)·h, and the addition of 0.2% 2‐phenylethanol increased the ethanol yield by up to 7.4%. These results demonstrate the feasibility of novel strategies to achieve sustainability goals in biomass conversions. John Wiley and Sons Inc. 2021-11-10 /pmc/articles/PMC8961051/ /pubmed/35382533 http://dx.doi.org/10.1002/elsc.202100095 Text en © 2021 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Huang, Xing‐Feng
Reardon, Kenneth F.
Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate
title Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate
title_full Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate
title_fullStr Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate
title_full_unstemmed Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate
title_short Strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate
title_sort strategies to achieve high productivity, high conversion, and high yield in yeast fermentation of algal biomass hydrolysate
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961051/
https://www.ncbi.nlm.nih.gov/pubmed/35382533
http://dx.doi.org/10.1002/elsc.202100095
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