Cargando…

Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment

To solve the issue of unsatisfactory recruitment of mesenchymal stem cells (MSCs) around implant in osteoporotic fractures, we fabricated a ROS-responsive system on titanium surface through hydroxyapatite coating and biomolecule grafting. The porous hydroxyapatite and phosphorylated osteogenic growt...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Maohua, Sun, Yuting, Hou, Yanhua, Luo, Zhong, Li, Menghuan, Wei, Yujia, Chen, Maowen, Tan, Lu, Cai, Kaiyong, Hu, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961459/
https://www.ncbi.nlm.nih.gov/pubmed/35387165
http://dx.doi.org/10.1016/j.bioactmat.2022.02.006
_version_ 1784677599798099968
author Chen, Maohua
Sun, Yuting
Hou, Yanhua
Luo, Zhong
Li, Menghuan
Wei, Yujia
Chen, Maowen
Tan, Lu
Cai, Kaiyong
Hu, Yan
author_facet Chen, Maohua
Sun, Yuting
Hou, Yanhua
Luo, Zhong
Li, Menghuan
Wei, Yujia
Chen, Maowen
Tan, Lu
Cai, Kaiyong
Hu, Yan
author_sort Chen, Maohua
collection PubMed
description To solve the issue of unsatisfactory recruitment of mesenchymal stem cells (MSCs) around implant in osteoporotic fractures, we fabricated a ROS-responsive system on titanium surface through hydroxyapatite coating and biomolecule grafting. The porous hydroxyapatite and phosphorylated osteogenic growth peptides (p-OGP) were introduced onto titanium surface to synergistically improve osteogenic differentiation of MSCs. After the p-OGP-promoted expression of osteogenic related proteins, the calcium and phosphate ions were released through the degradation of hydroxyapatite and integrated into bone tissues to boost the mineralization of bone matrix. The ROS-triggered release of DNA aptamer (Apt) 19S in the osteoporotic microenvironment guides MSC migration to implant site due to its high affinity with alkaline phosphatase on the membrane of MSCs. Once MSCs reached the implant interface, their osteogenic differentiation potential was enhanced by p-OGP and hydroxyapatite to promote bone regeneration. The study here provided a simple and novel strategy to prepare functional titanium implants for osteoporotic bone fracture repair.
format Online
Article
Text
id pubmed-8961459
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher KeAi Publishing
record_format MEDLINE/PubMed
spelling pubmed-89614592022-04-05 Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment Chen, Maohua Sun, Yuting Hou, Yanhua Luo, Zhong Li, Menghuan Wei, Yujia Chen, Maowen Tan, Lu Cai, Kaiyong Hu, Yan Bioact Mater Article To solve the issue of unsatisfactory recruitment of mesenchymal stem cells (MSCs) around implant in osteoporotic fractures, we fabricated a ROS-responsive system on titanium surface through hydroxyapatite coating and biomolecule grafting. The porous hydroxyapatite and phosphorylated osteogenic growth peptides (p-OGP) were introduced onto titanium surface to synergistically improve osteogenic differentiation of MSCs. After the p-OGP-promoted expression of osteogenic related proteins, the calcium and phosphate ions were released through the degradation of hydroxyapatite and integrated into bone tissues to boost the mineralization of bone matrix. The ROS-triggered release of DNA aptamer (Apt) 19S in the osteoporotic microenvironment guides MSC migration to implant site due to its high affinity with alkaline phosphatase on the membrane of MSCs. Once MSCs reached the implant interface, their osteogenic differentiation potential was enhanced by p-OGP and hydroxyapatite to promote bone regeneration. The study here provided a simple and novel strategy to prepare functional titanium implants for osteoporotic bone fracture repair. KeAi Publishing 2022-02-19 /pmc/articles/PMC8961459/ /pubmed/35387165 http://dx.doi.org/10.1016/j.bioactmat.2022.02.006 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Chen, Maohua
Sun, Yuting
Hou, Yanhua
Luo, Zhong
Li, Menghuan
Wei, Yujia
Chen, Maowen
Tan, Lu
Cai, Kaiyong
Hu, Yan
Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment
title Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment
title_full Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment
title_fullStr Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment
title_full_unstemmed Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment
title_short Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment
title_sort constructions of ros-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961459/
https://www.ncbi.nlm.nih.gov/pubmed/35387165
http://dx.doi.org/10.1016/j.bioactmat.2022.02.006
work_keys_str_mv AT chenmaohua constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT sunyuting constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT houyanhua constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT luozhong constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT limenghuan constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT weiyujia constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT chenmaowen constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT tanlu constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT caikaiyong constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment
AT huyan constructionsofrosresponsivetitaniumhydroxyapatiteimplantformesenchymalstemcellrecruitmentinperiimplantspaceandboneformationinosteoporosismicroenvironment