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Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice

BMAL1 is a core mammalian circadian clock transcription factor responsible for the regulation of the expression of thousands of genes. Previously, male skeletal-muscle-specific BMAL1-inducible-knockout (iMS-BMAL1 KO) mice have been described as a model that exhibits an aging-like phenotype with an a...

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Autores principales: Crislip, Gene Ryan, Wohlgemuth, Stephanie E., Wolff, Christopher A., Gutierrez-Monreal, Miguel A., Douglas, Collin M., Ebrahimi, Elnaz, Cheng, Kit-Yan, Masten, Sarah H., Barral, Dominique, Bryant, Andrew J., Esser, Karyn A., Gumz, Michelle L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961518/
https://www.ncbi.nlm.nih.gov/pubmed/35204763
http://dx.doi.org/10.3390/biom12020261
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author Crislip, Gene Ryan
Wohlgemuth, Stephanie E.
Wolff, Christopher A.
Gutierrez-Monreal, Miguel A.
Douglas, Collin M.
Ebrahimi, Elnaz
Cheng, Kit-Yan
Masten, Sarah H.
Barral, Dominique
Bryant, Andrew J.
Esser, Karyn A.
Gumz, Michelle L.
author_facet Crislip, Gene Ryan
Wohlgemuth, Stephanie E.
Wolff, Christopher A.
Gutierrez-Monreal, Miguel A.
Douglas, Collin M.
Ebrahimi, Elnaz
Cheng, Kit-Yan
Masten, Sarah H.
Barral, Dominique
Bryant, Andrew J.
Esser, Karyn A.
Gumz, Michelle L.
author_sort Crislip, Gene Ryan
collection PubMed
description BMAL1 is a core mammalian circadian clock transcription factor responsible for the regulation of the expression of thousands of genes. Previously, male skeletal-muscle-specific BMAL1-inducible-knockout (iMS-BMAL1 KO) mice have been described as a model that exhibits an aging-like phenotype with an altered gait, reduced mobility, muscle weakness, and impaired glucose uptake. Given this aging phenotype and that chronic kidney disease is a disease of aging, the goal of this study was to determine if iMS-BMAL1 KO mice exhibit a renal phenotype. Male iMS-BMAL1 KO and control mice were challenged with a low potassium diet for five days. Both genotypes responded appropriately by conserving urinary potassium. The iMS-BMAL1 KO mice excreted less potassium during the rest phase during the normal diet but there was no genotype difference during the active phase. Next, iMS-BMAL1 KO and control mice were used to compare markers of kidney injury and assess renal function before and after a phase advance protocol. Following phase advance, no differences were detected in renal mitochondrial function in iMS-BMAL1 KO mice compared to control mice. Additionally, the glomerular filtration rate and renal morphology were similar between groups in response to phase advance. Disruption of the clock in skeletal muscle tissue activates inflammatory pathways within the kidney of male mice, and there is evidence of this affecting other organs, such as the lungs. However, there were no signs of renal injury or altered function following clock disruption of skeletal muscle under the conditions tested.
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spelling pubmed-89615182022-03-30 Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice Crislip, Gene Ryan Wohlgemuth, Stephanie E. Wolff, Christopher A. Gutierrez-Monreal, Miguel A. Douglas, Collin M. Ebrahimi, Elnaz Cheng, Kit-Yan Masten, Sarah H. Barral, Dominique Bryant, Andrew J. Esser, Karyn A. Gumz, Michelle L. Biomolecules Article BMAL1 is a core mammalian circadian clock transcription factor responsible for the regulation of the expression of thousands of genes. Previously, male skeletal-muscle-specific BMAL1-inducible-knockout (iMS-BMAL1 KO) mice have been described as a model that exhibits an aging-like phenotype with an altered gait, reduced mobility, muscle weakness, and impaired glucose uptake. Given this aging phenotype and that chronic kidney disease is a disease of aging, the goal of this study was to determine if iMS-BMAL1 KO mice exhibit a renal phenotype. Male iMS-BMAL1 KO and control mice were challenged with a low potassium diet for five days. Both genotypes responded appropriately by conserving urinary potassium. The iMS-BMAL1 KO mice excreted less potassium during the rest phase during the normal diet but there was no genotype difference during the active phase. Next, iMS-BMAL1 KO and control mice were used to compare markers of kidney injury and assess renal function before and after a phase advance protocol. Following phase advance, no differences were detected in renal mitochondrial function in iMS-BMAL1 KO mice compared to control mice. Additionally, the glomerular filtration rate and renal morphology were similar between groups in response to phase advance. Disruption of the clock in skeletal muscle tissue activates inflammatory pathways within the kidney of male mice, and there is evidence of this affecting other organs, such as the lungs. However, there were no signs of renal injury or altered function following clock disruption of skeletal muscle under the conditions tested. MDPI 2022-02-05 /pmc/articles/PMC8961518/ /pubmed/35204763 http://dx.doi.org/10.3390/biom12020261 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Crislip, Gene Ryan
Wohlgemuth, Stephanie E.
Wolff, Christopher A.
Gutierrez-Monreal, Miguel A.
Douglas, Collin M.
Ebrahimi, Elnaz
Cheng, Kit-Yan
Masten, Sarah H.
Barral, Dominique
Bryant, Andrew J.
Esser, Karyn A.
Gumz, Michelle L.
Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice
title Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice
title_full Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice
title_fullStr Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice
title_full_unstemmed Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice
title_short Apparent Absence of BMAL1-Dependent Skeletal Muscle–Kidney Cross Talk in Mice
title_sort apparent absence of bmal1-dependent skeletal muscle–kidney cross talk in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961518/
https://www.ncbi.nlm.nih.gov/pubmed/35204763
http://dx.doi.org/10.3390/biom12020261
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