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miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find tha...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961554/ https://www.ncbi.nlm.nih.gov/pubmed/35204692 http://dx.doi.org/10.3390/biom12020191 |
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author | Yang, Xin Li, Zhenhui Wang, Zhijun Yu, Jiaao Ma, Manting Nie, Qinghua |
author_facet | Yang, Xin Li, Zhenhui Wang, Zhijun Yu, Jiaao Ma, Manting Nie, Qinghua |
author_sort | Yang, Xin |
collection | PubMed |
description | As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find that miR-27b-3p and Cbl-b genes are significantly differentially expressed in the induced atrophy model. The dual-luciferase experiment and Western blot analysis confirmed that miR-27b-3p could regulate the expression of Cbl-b. In C2C12-differentiated myotubes, the overexpression of the Cbl-b gene showed that Cbl-b could upregulate the expression of MuRF-1 and Atrogin-1, which are related marker genes of muscle atrophy, at both the mRNA and protein levels, indicating that the Cbl-b gene can specifically affect muscle atrophy. The knockdown of the Cbl-b gene after C2C12-differentiated myotubes induced atrophy treatment can downregulate the expression of muscle-atrophy-related genes, indicating that manual intervention to downregulate the expression of Cbl-b has a certain alleviating effect on muscle atrophy. These data suggest that miR-27b-3p can regulate the expression of the Cbl-b gene and then exert a particular influence on muscle atrophy through the Cbl-b gene. |
format | Online Article Text |
id | pubmed-8961554 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-89615542022-03-30 miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles Yang, Xin Li, Zhenhui Wang, Zhijun Yu, Jiaao Ma, Manting Nie, Qinghua Biomolecules Article As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find that miR-27b-3p and Cbl-b genes are significantly differentially expressed in the induced atrophy model. The dual-luciferase experiment and Western blot analysis confirmed that miR-27b-3p could regulate the expression of Cbl-b. In C2C12-differentiated myotubes, the overexpression of the Cbl-b gene showed that Cbl-b could upregulate the expression of MuRF-1 and Atrogin-1, which are related marker genes of muscle atrophy, at both the mRNA and protein levels, indicating that the Cbl-b gene can specifically affect muscle atrophy. The knockdown of the Cbl-b gene after C2C12-differentiated myotubes induced atrophy treatment can downregulate the expression of muscle-atrophy-related genes, indicating that manual intervention to downregulate the expression of Cbl-b has a certain alleviating effect on muscle atrophy. These data suggest that miR-27b-3p can regulate the expression of the Cbl-b gene and then exert a particular influence on muscle atrophy through the Cbl-b gene. MDPI 2022-01-23 /pmc/articles/PMC8961554/ /pubmed/35204692 http://dx.doi.org/10.3390/biom12020191 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Xin Li, Zhenhui Wang, Zhijun Yu, Jiaao Ma, Manting Nie, Qinghua miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles |
title | miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles |
title_full | miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles |
title_fullStr | miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles |
title_full_unstemmed | miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles |
title_short | miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles |
title_sort | mir-27b-3p attenuates muscle atrophy by targeting cbl-b in skeletal muscles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961554/ https://www.ncbi.nlm.nih.gov/pubmed/35204692 http://dx.doi.org/10.3390/biom12020191 |
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