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miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles

As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find tha...

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Autores principales: Yang, Xin, Li, Zhenhui, Wang, Zhijun, Yu, Jiaao, Ma, Manting, Nie, Qinghua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961554/
https://www.ncbi.nlm.nih.gov/pubmed/35204692
http://dx.doi.org/10.3390/biom12020191
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author Yang, Xin
Li, Zhenhui
Wang, Zhijun
Yu, Jiaao
Ma, Manting
Nie, Qinghua
author_facet Yang, Xin
Li, Zhenhui
Wang, Zhijun
Yu, Jiaao
Ma, Manting
Nie, Qinghua
author_sort Yang, Xin
collection PubMed
description As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find that miR-27b-3p and Cbl-b genes are significantly differentially expressed in the induced atrophy model. The dual-luciferase experiment and Western blot analysis confirmed that miR-27b-3p could regulate the expression of Cbl-b. In C2C12-differentiated myotubes, the overexpression of the Cbl-b gene showed that Cbl-b could upregulate the expression of MuRF-1 and Atrogin-1, which are related marker genes of muscle atrophy, at both the mRNA and protein levels, indicating that the Cbl-b gene can specifically affect muscle atrophy. The knockdown of the Cbl-b gene after C2C12-differentiated myotubes induced atrophy treatment can downregulate the expression of muscle-atrophy-related genes, indicating that manual intervention to downregulate the expression of Cbl-b has a certain alleviating effect on muscle atrophy. These data suggest that miR-27b-3p can regulate the expression of the Cbl-b gene and then exert a particular influence on muscle atrophy through the Cbl-b gene.
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spelling pubmed-89615542022-03-30 miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles Yang, Xin Li, Zhenhui Wang, Zhijun Yu, Jiaao Ma, Manting Nie, Qinghua Biomolecules Article As it is well known, muscle atrophy is a process in which protein degradation increases and protein synthesis decreases. This process is regulated by a variety of links. Among them, microRNAs play an essential role in this process, which has attracted widespread attention. In this paper, we find that miR-27b-3p and Cbl-b genes are significantly differentially expressed in the induced atrophy model. The dual-luciferase experiment and Western blot analysis confirmed that miR-27b-3p could regulate the expression of Cbl-b. In C2C12-differentiated myotubes, the overexpression of the Cbl-b gene showed that Cbl-b could upregulate the expression of MuRF-1 and Atrogin-1, which are related marker genes of muscle atrophy, at both the mRNA and protein levels, indicating that the Cbl-b gene can specifically affect muscle atrophy. The knockdown of the Cbl-b gene after C2C12-differentiated myotubes induced atrophy treatment can downregulate the expression of muscle-atrophy-related genes, indicating that manual intervention to downregulate the expression of Cbl-b has a certain alleviating effect on muscle atrophy. These data suggest that miR-27b-3p can regulate the expression of the Cbl-b gene and then exert a particular influence on muscle atrophy through the Cbl-b gene. MDPI 2022-01-23 /pmc/articles/PMC8961554/ /pubmed/35204692 http://dx.doi.org/10.3390/biom12020191 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Xin
Li, Zhenhui
Wang, Zhijun
Yu, Jiaao
Ma, Manting
Nie, Qinghua
miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
title miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
title_full miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
title_fullStr miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
title_full_unstemmed miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
title_short miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles
title_sort mir-27b-3p attenuates muscle atrophy by targeting cbl-b in skeletal muscles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961554/
https://www.ncbi.nlm.nih.gov/pubmed/35204692
http://dx.doi.org/10.3390/biom12020191
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