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Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia

Chronic inflammation is characterized by the production of reactive oxygen species (ROS), reactive nitrogen species, and inflammatory cytokines in myeloproliferative neoplasms (MPNs). In addition to these parameters, the aim of this study was to analyze the influence of ROS on the proliferation-rela...

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Autores principales: Đikić, Dragoslava, Bogdanović, Andrija, Marković, Dragana, Mitrović-Ajtić, Olivera, Subotički, Tijana, Diklić, Miloš, Vukotić, Milica, Dragojević, Teodora, Živković, Emilija, Santibanez, Juan F., Čokić, Vladan P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961589/
https://www.ncbi.nlm.nih.gov/pubmed/35204748
http://dx.doi.org/10.3390/biom12020247
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author Đikić, Dragoslava
Bogdanović, Andrija
Marković, Dragana
Mitrović-Ajtić, Olivera
Subotički, Tijana
Diklić, Miloš
Vukotić, Milica
Dragojević, Teodora
Živković, Emilija
Santibanez, Juan F.
Čokić, Vladan P.
author_facet Đikić, Dragoslava
Bogdanović, Andrija
Marković, Dragana
Mitrović-Ajtić, Olivera
Subotički, Tijana
Diklić, Miloš
Vukotić, Milica
Dragojević, Teodora
Živković, Emilija
Santibanez, Juan F.
Čokić, Vladan P.
author_sort Đikić, Dragoslava
collection PubMed
description Chronic inflammation is characterized by the production of reactive oxygen species (ROS), reactive nitrogen species, and inflammatory cytokines in myeloproliferative neoplasms (MPNs). In addition to these parameters, the aim of this study was to analyze the influence of ROS on the proliferation-related AKT/mTOR signaling pathway and the relationship with inflammatory factors in chronic myelogenous leukemia (CML). The activity of the antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase is reduced in erythrocytes while levels of the oxidative stress markers malondialdehyde and protein carbonyl are elevated in the plasma of patients with CML. In addition, nitrogen species (nitrotyrosine, iNOS, eNOS) and inflammation markers (IL-6, NFkB, and S100 protein) were increased in granulocytes of CML while anti-inflammatory levels of IL-10 were decreased in plasma. CML granulocytes exhibited greater resistance to cytotoxic H(2)O(2) activity compared to healthy subjects. Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H(2)O(2)) in granulocytes and erythroleukemic K562 cells. IL-6 caused oxidative stress and DNA damage that was mitigated using antioxidant or inhibition of inflammatory NFkB transcription factor in K562 cells. We demonstrated the presence of oxidative and nitrosative stress in CML, with the former mediated by AKT/mTOR signaling and stimulated by inflammation.
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spelling pubmed-89615892022-03-30 Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia Đikić, Dragoslava Bogdanović, Andrija Marković, Dragana Mitrović-Ajtić, Olivera Subotički, Tijana Diklić, Miloš Vukotić, Milica Dragojević, Teodora Živković, Emilija Santibanez, Juan F. Čokić, Vladan P. Biomolecules Article Chronic inflammation is characterized by the production of reactive oxygen species (ROS), reactive nitrogen species, and inflammatory cytokines in myeloproliferative neoplasms (MPNs). In addition to these parameters, the aim of this study was to analyze the influence of ROS on the proliferation-related AKT/mTOR signaling pathway and the relationship with inflammatory factors in chronic myelogenous leukemia (CML). The activity of the antioxidant enzymes superoxide dismutase, glutathione peroxidase, and catalase is reduced in erythrocytes while levels of the oxidative stress markers malondialdehyde and protein carbonyl are elevated in the plasma of patients with CML. In addition, nitrogen species (nitrotyrosine, iNOS, eNOS) and inflammation markers (IL-6, NFkB, and S100 protein) were increased in granulocytes of CML while anti-inflammatory levels of IL-10 were decreased in plasma. CML granulocytes exhibited greater resistance to cytotoxic H(2)O(2) activity compared to healthy subjects. Moreover, phosphorylation of the apoptotic p53 protein was reduced while the activity of the AKT/mTOR signaling pathway was increased, which was further enhanced by oxidative stress (H(2)O(2)) in granulocytes and erythroleukemic K562 cells. IL-6 caused oxidative stress and DNA damage that was mitigated using antioxidant or inhibition of inflammatory NFkB transcription factor in K562 cells. We demonstrated the presence of oxidative and nitrosative stress in CML, with the former mediated by AKT/mTOR signaling and stimulated by inflammation. MDPI 2022-02-03 /pmc/articles/PMC8961589/ /pubmed/35204748 http://dx.doi.org/10.3390/biom12020247 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Đikić, Dragoslava
Bogdanović, Andrija
Marković, Dragana
Mitrović-Ajtić, Olivera
Subotički, Tijana
Diklić, Miloš
Vukotić, Milica
Dragojević, Teodora
Živković, Emilija
Santibanez, Juan F.
Čokić, Vladan P.
Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
title Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
title_full Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
title_fullStr Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
title_full_unstemmed Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
title_short Inflammation Promotes Oxidative and Nitrosative Stress in Chronic Myelogenous Leukemia
title_sort inflammation promotes oxidative and nitrosative stress in chronic myelogenous leukemia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961589/
https://www.ncbi.nlm.nih.gov/pubmed/35204748
http://dx.doi.org/10.3390/biom12020247
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