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Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus

Staphylococcus aureus remains a pathogen of high concern in public health programs worldwide due to antibiotic resistance and emergence of highly virulent strains. Many phytochemicals have demonstrated activity against S. aureus and other Gram-positive bacteria, but the minimum inhibitory concentrat...

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Autores principales: Pájaro-González, Yina, Oliveros-Díaz, Andrés F., Cabrera-Barraza, Julián, Fernández-Daza, Eduardo, Reyes, Niradiz, Montes-Guevara, Oscar A., Caro-Fuentes, Daneiva, Franco-Ospina, Luis, Quiñones- Fletcher, Wiston, Quave, Cassandra L., Díaz-Castillo, Fredyc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961693/
https://www.ncbi.nlm.nih.gov/pubmed/35359842
http://dx.doi.org/10.3389/fphar.2022.826404
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author Pájaro-González, Yina
Oliveros-Díaz, Andrés F.
Cabrera-Barraza, Julián
Fernández-Daza, Eduardo
Reyes, Niradiz
Montes-Guevara, Oscar A.
Caro-Fuentes, Daneiva
Franco-Ospina, Luis
Quiñones- Fletcher, Wiston
Quave, Cassandra L.
Díaz-Castillo, Fredyc
author_facet Pájaro-González, Yina
Oliveros-Díaz, Andrés F.
Cabrera-Barraza, Julián
Fernández-Daza, Eduardo
Reyes, Niradiz
Montes-Guevara, Oscar A.
Caro-Fuentes, Daneiva
Franco-Ospina, Luis
Quiñones- Fletcher, Wiston
Quave, Cassandra L.
Díaz-Castillo, Fredyc
author_sort Pájaro-González, Yina
collection PubMed
description Staphylococcus aureus remains a pathogen of high concern in public health programs worldwide due to antibiotic resistance and emergence of highly virulent strains. Many phytochemicals have demonstrated activity against S. aureus and other Gram-positive bacteria, but the minimum inhibitory concentration (MIC) values comparable to commonly used antibiotics are needed. In the present study, bio-guided fractionation of the ethanol extract of seeds of Mammea americana L. (Calophyllaceae) throughout the antibacterial activity, against S. aureus strains that are sensitive and resistant to methicillin, led to the isolation of four coumarins identified as mammea B/BA, mammea B/BC, mammea A/AA cyclo D and mammea A/AA cyclo F, and a mixture of mammea B/BA cyclo F plus mammea B/BD cyclo F. The extract inhibited the growth of S. aureus with MIC values of 2–4 μg/ml and Mammea B/BA (MaBBA) presented MIC values in a range between 0.5 and 1.0 μg/ml in six methicillin-sensitive strains and eight methicillin-resistant strains evaluated. We consider MaBBA the most potent of all mammea coumarins reported to date, according to the literature review carried out at the time of writing of this article. Toxicity assessment in vivo against the nematode Caenorhabditis elegans and in vitro against human fibroblasts of the extract and the compound MaBBA indicated that both had low toxicity.
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spelling pubmed-89616932022-03-30 Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus Pájaro-González, Yina Oliveros-Díaz, Andrés F. Cabrera-Barraza, Julián Fernández-Daza, Eduardo Reyes, Niradiz Montes-Guevara, Oscar A. Caro-Fuentes, Daneiva Franco-Ospina, Luis Quiñones- Fletcher, Wiston Quave, Cassandra L. Díaz-Castillo, Fredyc Front Pharmacol Pharmacology Staphylococcus aureus remains a pathogen of high concern in public health programs worldwide due to antibiotic resistance and emergence of highly virulent strains. Many phytochemicals have demonstrated activity against S. aureus and other Gram-positive bacteria, but the minimum inhibitory concentration (MIC) values comparable to commonly used antibiotics are needed. In the present study, bio-guided fractionation of the ethanol extract of seeds of Mammea americana L. (Calophyllaceae) throughout the antibacterial activity, against S. aureus strains that are sensitive and resistant to methicillin, led to the isolation of four coumarins identified as mammea B/BA, mammea B/BC, mammea A/AA cyclo D and mammea A/AA cyclo F, and a mixture of mammea B/BA cyclo F plus mammea B/BD cyclo F. The extract inhibited the growth of S. aureus with MIC values of 2–4 μg/ml and Mammea B/BA (MaBBA) presented MIC values in a range between 0.5 and 1.0 μg/ml in six methicillin-sensitive strains and eight methicillin-resistant strains evaluated. We consider MaBBA the most potent of all mammea coumarins reported to date, according to the literature review carried out at the time of writing of this article. Toxicity assessment in vivo against the nematode Caenorhabditis elegans and in vitro against human fibroblasts of the extract and the compound MaBBA indicated that both had low toxicity. Frontiers Media S.A. 2022-03-11 /pmc/articles/PMC8961693/ /pubmed/35359842 http://dx.doi.org/10.3389/fphar.2022.826404 Text en Copyright © 2022 Pájaro-González, Oliveros-Díaz, Cabrera-Barraza, Fernández-Daza, Reyes, Montes-Guevara, Caro-Fuentes, Franco-Ospina, Quiñones- Fletcher, Quave and Díaz-Castillo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Pájaro-González, Yina
Oliveros-Díaz, Andrés F.
Cabrera-Barraza, Julián
Fernández-Daza, Eduardo
Reyes, Niradiz
Montes-Guevara, Oscar A.
Caro-Fuentes, Daneiva
Franco-Ospina, Luis
Quiñones- Fletcher, Wiston
Quave, Cassandra L.
Díaz-Castillo, Fredyc
Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus
title Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus
title_full Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus
title_fullStr Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus
title_full_unstemmed Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus
title_short Mammea B/BA Isolated From the Seeds of Mammea americana L. (Calophyllaceae) is a Potent Inhibitor of Methicillin-Resistant Staphylococcus aureus
title_sort mammea b/ba isolated from the seeds of mammea americana l. (calophyllaceae) is a potent inhibitor of methicillin-resistant staphylococcus aureus
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961693/
https://www.ncbi.nlm.nih.gov/pubmed/35359842
http://dx.doi.org/10.3389/fphar.2022.826404
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