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Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy

BACKGROUND: Analysis of the constituents of gallstones using various spectroscopic techniques assists in identification of the pathogenesis of gallstones. In the current study, using Fourier Transform Infra-Red (FTIR) Spectroscopy, a Gallstone Standard Library (GSL) and a Gallstone Real Patients’ Li...

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Autores principales: Jafri, Lena, Abid, Muhammad Abbas, Asif, Humera, Hashmi, Bilal, Majid, Hafsa, Ghani, Farooq, Ahmed, Sibtain, Siddiqui, Imran, Khan, Aysha Habib
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962173/
https://www.ncbi.nlm.nih.gov/pubmed/35346074
http://dx.doi.org/10.1186/s12876-022-02227-8
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author Jafri, Lena
Abid, Muhammad Abbas
Asif, Humera
Hashmi, Bilal
Majid, Hafsa
Ghani, Farooq
Ahmed, Sibtain
Siddiqui, Imran
Khan, Aysha Habib
author_facet Jafri, Lena
Abid, Muhammad Abbas
Asif, Humera
Hashmi, Bilal
Majid, Hafsa
Ghani, Farooq
Ahmed, Sibtain
Siddiqui, Imran
Khan, Aysha Habib
author_sort Jafri, Lena
collection PubMed
description BACKGROUND: Analysis of the constituents of gallstones using various spectroscopic techniques assists in identification of the pathogenesis of gallstones. In the current study, using Fourier Transform Infra-Red (FTIR) Spectroscopy, a Gallstone Standard Library (GSL) and a Gallstone Real Patients’ Library (GRPL) were developed and validated for gallstone composition analysis. METHODS: The study was conducted at the Department of Pathology & Laboratory Medicine, Aga Khan University, Pakistan. Pure standards (cholesterol, calcium carbonate, bilirubin and bile salts) and gallstone specimens were analyzed using FTIR Nicolet iS-5 Spectrometer from Thermo Fisher Scientific, USA. Thermo Scientific™ QCheck™ algorithm, embedded within the OMNIC™ software, was used to identify the unique spectral fingerprint of the patient samples to match with known, standard material. Matching of > 75% was considered acceptable. Validation for accuracy of the library was performed for twenty analyzed gallstones at an international reference lab. RESULTS: Concerted search analysis was performed against the developed GSL consisting of 71 “pure component” spectrum divided into 5 types to generate the library. For the Gallstone Real Patient Library (GRPL), 117 patient samples were analyzed. Ninety-eight gall stones (83.8%) out of 117 stones matched with the developed GSL. Majority stones were mixed stones (95.92%), with cholesterol being the primary component (91.83%). Results of the developed library were 100% in agreement with the reports received from the external reference lab. CONCLUSIONS: The library developed displayed good consistency and can be used for detection of gallstone composition in Pakistan and replace the traditional labor- and time-intensive chemical method of gallstone analysis.
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spelling pubmed-89621732022-03-30 Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy Jafri, Lena Abid, Muhammad Abbas Asif, Humera Hashmi, Bilal Majid, Hafsa Ghani, Farooq Ahmed, Sibtain Siddiqui, Imran Khan, Aysha Habib BMC Gastroenterol Research BACKGROUND: Analysis of the constituents of gallstones using various spectroscopic techniques assists in identification of the pathogenesis of gallstones. In the current study, using Fourier Transform Infra-Red (FTIR) Spectroscopy, a Gallstone Standard Library (GSL) and a Gallstone Real Patients’ Library (GRPL) were developed and validated for gallstone composition analysis. METHODS: The study was conducted at the Department of Pathology & Laboratory Medicine, Aga Khan University, Pakistan. Pure standards (cholesterol, calcium carbonate, bilirubin and bile salts) and gallstone specimens were analyzed using FTIR Nicolet iS-5 Spectrometer from Thermo Fisher Scientific, USA. Thermo Scientific™ QCheck™ algorithm, embedded within the OMNIC™ software, was used to identify the unique spectral fingerprint of the patient samples to match with known, standard material. Matching of > 75% was considered acceptable. Validation for accuracy of the library was performed for twenty analyzed gallstones at an international reference lab. RESULTS: Concerted search analysis was performed against the developed GSL consisting of 71 “pure component” spectrum divided into 5 types to generate the library. For the Gallstone Real Patient Library (GRPL), 117 patient samples were analyzed. Ninety-eight gall stones (83.8%) out of 117 stones matched with the developed GSL. Majority stones were mixed stones (95.92%), with cholesterol being the primary component (91.83%). Results of the developed library were 100% in agreement with the reports received from the external reference lab. CONCLUSIONS: The library developed displayed good consistency and can be used for detection of gallstone composition in Pakistan and replace the traditional labor- and time-intensive chemical method of gallstone analysis. BioMed Central 2022-03-28 /pmc/articles/PMC8962173/ /pubmed/35346074 http://dx.doi.org/10.1186/s12876-022-02227-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Jafri, Lena
Abid, Muhammad Abbas
Asif, Humera
Hashmi, Bilal
Majid, Hafsa
Ghani, Farooq
Ahmed, Sibtain
Siddiqui, Imran
Khan, Aysha Habib
Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy
title Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy
title_full Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy
title_fullStr Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy
title_full_unstemmed Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy
title_short Development and validation of standard and real patient gallstone library using Fourier transform infra-red spectroscopy
title_sort development and validation of standard and real patient gallstone library using fourier transform infra-red spectroscopy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962173/
https://www.ncbi.nlm.nih.gov/pubmed/35346074
http://dx.doi.org/10.1186/s12876-022-02227-8
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