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Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads

Hypoparathyroidism is an endocrine disorder that occurs because of the inability to produce parathyroid hormone (PTH) effectively. Previously, we reported the efficacy of tonsil-derived mesenchymal stem cells (TMSCs) differentiated into parathyroid-like cells for the treatment of hypoparathyroidism....

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Autores principales: Kim, Ha Yeong, Seok, Ji Min, Jung, Soo Yeon, Lee, Min Ji, Tran, An Nguyen-Thuy, Yeo, Seon Ju, Park, Su A., Kim, Han Su
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962264/
https://www.ncbi.nlm.nih.gov/pubmed/35203648
http://dx.doi.org/10.3390/biomedicines10020440
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author Kim, Ha Yeong
Seok, Ji Min
Jung, Soo Yeon
Lee, Min Ji
Tran, An Nguyen-Thuy
Yeo, Seon Ju
Park, Su A.
Kim, Han Su
author_facet Kim, Ha Yeong
Seok, Ji Min
Jung, Soo Yeon
Lee, Min Ji
Tran, An Nguyen-Thuy
Yeo, Seon Ju
Park, Su A.
Kim, Han Su
author_sort Kim, Ha Yeong
collection PubMed
description Hypoparathyroidism is an endocrine disorder that occurs because of the inability to produce parathyroid hormone (PTH) effectively. Previously, we reported the efficacy of tonsil-derived mesenchymal stem cells (TMSCs) differentiated into parathyroid-like cells for the treatment of hypoparathyroidism. Here, we investigated the feasibility of three-dimensional structural microbeads fabricated with TMSCs and alginate, a natural biodegradable polymer, to treat hypoparathyroidism. Alginate microbeads were fabricated by dropping a 2% (w/v) alginate solution containing TMSCs into a 5% CaCl(2) solution and then differentiated into parathyroid-like cells using activin A and sonic hedgehog for 7 days. The protein expression of PTH, a specific marker of the parathyroid gland, was significantly higher in differentiated alginate microbeads with TMSCs (Al-dT) compared with in undifferentiated alginate microbeads with TMSCs. For in vivo experiments, we created the hypoparathyroidism animal model by parathyroidectomy (PTX) and implanted alginate microbeads in the dorsal interscapular region. The PTX rats with Al-dT (PTX+Al-dT) showed the highest survival rate and weight change and a gradual increase in serum intact PTH levels. We also detected a higher expression of PTH in retrieved tissues of PTX+Al-dT using immunofluorescence analysis. This study demonstrates that alginate microbeads are potential a new tool as a surgically scalable therapy for treating hypoparathyroidism.
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spelling pubmed-89622642022-03-30 Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads Kim, Ha Yeong Seok, Ji Min Jung, Soo Yeon Lee, Min Ji Tran, An Nguyen-Thuy Yeo, Seon Ju Park, Su A. Kim, Han Su Biomedicines Article Hypoparathyroidism is an endocrine disorder that occurs because of the inability to produce parathyroid hormone (PTH) effectively. Previously, we reported the efficacy of tonsil-derived mesenchymal stem cells (TMSCs) differentiated into parathyroid-like cells for the treatment of hypoparathyroidism. Here, we investigated the feasibility of three-dimensional structural microbeads fabricated with TMSCs and alginate, a natural biodegradable polymer, to treat hypoparathyroidism. Alginate microbeads were fabricated by dropping a 2% (w/v) alginate solution containing TMSCs into a 5% CaCl(2) solution and then differentiated into parathyroid-like cells using activin A and sonic hedgehog for 7 days. The protein expression of PTH, a specific marker of the parathyroid gland, was significantly higher in differentiated alginate microbeads with TMSCs (Al-dT) compared with in undifferentiated alginate microbeads with TMSCs. For in vivo experiments, we created the hypoparathyroidism animal model by parathyroidectomy (PTX) and implanted alginate microbeads in the dorsal interscapular region. The PTX rats with Al-dT (PTX+Al-dT) showed the highest survival rate and weight change and a gradual increase in serum intact PTH levels. We also detected a higher expression of PTH in retrieved tissues of PTX+Al-dT using immunofluorescence analysis. This study demonstrates that alginate microbeads are potential a new tool as a surgically scalable therapy for treating hypoparathyroidism. MDPI 2022-02-14 /pmc/articles/PMC8962264/ /pubmed/35203648 http://dx.doi.org/10.3390/biomedicines10020440 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Ha Yeong
Seok, Ji Min
Jung, Soo Yeon
Lee, Min Ji
Tran, An Nguyen-Thuy
Yeo, Seon Ju
Park, Su A.
Kim, Han Su
Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads
title Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads
title_full Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads
title_fullStr Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads
title_full_unstemmed Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads
title_short Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads
title_sort development of surgically transplantable parathyroid hormone-releasing microbeads
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962264/
https://www.ncbi.nlm.nih.gov/pubmed/35203648
http://dx.doi.org/10.3390/biomedicines10020440
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