Cargando…

Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis

Sepsis is associated with circulatory dysfunction contributing to disturbed blood flow and organ injury. Decreased organ perfusion in sepsis is attributed, in part, to the loss of vasoregulatory mechanisms. Identifying which vascular beds are most susceptible to dysfunction is important for monitori...

Descripción completa

Detalles Bibliográficos
Autores principales: Jahandideh, Forough, Panahi, Sareh, Noble, Ronan M. N., Gragasin, Ferrante S., Khadaroo, Rachel G., Macala, Kimberly F., Bourque, Stephane L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962278/
https://www.ncbi.nlm.nih.gov/pubmed/35203689
http://dx.doi.org/10.3390/biomedicines10020470
_version_ 1784677763696820224
author Jahandideh, Forough
Panahi, Sareh
Noble, Ronan M. N.
Gragasin, Ferrante S.
Khadaroo, Rachel G.
Macala, Kimberly F.
Bourque, Stephane L.
author_facet Jahandideh, Forough
Panahi, Sareh
Noble, Ronan M. N.
Gragasin, Ferrante S.
Khadaroo, Rachel G.
Macala, Kimberly F.
Bourque, Stephane L.
author_sort Jahandideh, Forough
collection PubMed
description Sepsis is associated with circulatory dysfunction contributing to disturbed blood flow and organ injury. Decreased organ perfusion in sepsis is attributed, in part, to the loss of vasoregulatory mechanisms. Identifying which vascular beds are most susceptible to dysfunction is important for monitoring the recovery of organ function and guiding interventions. This study aimed to investigate the development of vascular dysfunction as sepsis progressed to septic shock. Anesthetized C57Bl/6 mice were instrumented with a fiberoptic pressure sensor in the carotid artery for blood pressure measurements. In subgroups of mice, regional blood flow measurements were taken by positioning a perivascular flow probe around either the left carotid, left renal, or superior mesenteric arteries. Hemodynamic parameters and their responsiveness to bolus doses of vasoactive drugs were recorded prior to and continuously after injection of fecal slurry (1.3 mg/g body weight) for 4 h. Fecal slurry-induced peritonitis reduced mean arterial pressure (62.7 ± 2.4 mmHg vs. 37.5 ± 3.2 mmHg in vehicle and septic mice, respectively), impaired cardiac function, and eventually reduced organ blood flow (71.9%, 66.8%, and 65.1% in the superior mesenteric, renal, and carotid arteries, respectively). The mesenteric vasculature exhibited dysregulation before the renal and carotid arteries, and this underlying dysfunction preceded the blood pressure decline and impaired organ blood flow.
format Online
Article
Text
id pubmed-8962278
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-89622782022-03-30 Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis Jahandideh, Forough Panahi, Sareh Noble, Ronan M. N. Gragasin, Ferrante S. Khadaroo, Rachel G. Macala, Kimberly F. Bourque, Stephane L. Biomedicines Article Sepsis is associated with circulatory dysfunction contributing to disturbed blood flow and organ injury. Decreased organ perfusion in sepsis is attributed, in part, to the loss of vasoregulatory mechanisms. Identifying which vascular beds are most susceptible to dysfunction is important for monitoring the recovery of organ function and guiding interventions. This study aimed to investigate the development of vascular dysfunction as sepsis progressed to septic shock. Anesthetized C57Bl/6 mice were instrumented with a fiberoptic pressure sensor in the carotid artery for blood pressure measurements. In subgroups of mice, regional blood flow measurements were taken by positioning a perivascular flow probe around either the left carotid, left renal, or superior mesenteric arteries. Hemodynamic parameters and their responsiveness to bolus doses of vasoactive drugs were recorded prior to and continuously after injection of fecal slurry (1.3 mg/g body weight) for 4 h. Fecal slurry-induced peritonitis reduced mean arterial pressure (62.7 ± 2.4 mmHg vs. 37.5 ± 3.2 mmHg in vehicle and septic mice, respectively), impaired cardiac function, and eventually reduced organ blood flow (71.9%, 66.8%, and 65.1% in the superior mesenteric, renal, and carotid arteries, respectively). The mesenteric vasculature exhibited dysregulation before the renal and carotid arteries, and this underlying dysfunction preceded the blood pressure decline and impaired organ blood flow. MDPI 2022-02-18 /pmc/articles/PMC8962278/ /pubmed/35203689 http://dx.doi.org/10.3390/biomedicines10020470 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jahandideh, Forough
Panahi, Sareh
Noble, Ronan M. N.
Gragasin, Ferrante S.
Khadaroo, Rachel G.
Macala, Kimberly F.
Bourque, Stephane L.
Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis
title Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis
title_full Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis
title_fullStr Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis
title_full_unstemmed Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis
title_short Characterization of Systemic and Regional Hemodynamics and Vascular Dysfunction in Mice with Fecal Induced Peritonitis
title_sort characterization of systemic and regional hemodynamics and vascular dysfunction in mice with fecal induced peritonitis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962278/
https://www.ncbi.nlm.nih.gov/pubmed/35203689
http://dx.doi.org/10.3390/biomedicines10020470
work_keys_str_mv AT jahandidehforough characterizationofsystemicandregionalhemodynamicsandvasculardysfunctioninmicewithfecalinducedperitonitis
AT panahisareh characterizationofsystemicandregionalhemodynamicsandvasculardysfunctioninmicewithfecalinducedperitonitis
AT nobleronanmn characterizationofsystemicandregionalhemodynamicsandvasculardysfunctioninmicewithfecalinducedperitonitis
AT gragasinferrantes characterizationofsystemicandregionalhemodynamicsandvasculardysfunctioninmicewithfecalinducedperitonitis
AT khadaroorachelg characterizationofsystemicandregionalhemodynamicsandvasculardysfunctioninmicewithfecalinducedperitonitis
AT macalakimberlyf characterizationofsystemicandregionalhemodynamicsandvasculardysfunctioninmicewithfecalinducedperitonitis
AT bourquestephanel characterizationofsystemicandregionalhemodynamicsandvasculardysfunctioninmicewithfecalinducedperitonitis