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An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples

The constant dialogue between the plant world and the animal world (including man among them) has been known since the time of Adam and Eve, where an apple was the origin of the evils of the world. Apart from Snow White—who might have something to object to when it comes to the use of apples—fruits,...

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Autores principales: Trentini, Martina, Zanotti, Federica, Tiengo, Elena, Camponogara, Francesca, Degasperi, Margherita, Licastro, Danilo, Lovatti, Luca, Zavan, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962404/
https://www.ncbi.nlm.nih.gov/pubmed/35203624
http://dx.doi.org/10.3390/biomedicines10020415
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author Trentini, Martina
Zanotti, Federica
Tiengo, Elena
Camponogara, Francesca
Degasperi, Margherita
Licastro, Danilo
Lovatti, Luca
Zavan, Barbara
author_facet Trentini, Martina
Zanotti, Federica
Tiengo, Elena
Camponogara, Francesca
Degasperi, Margherita
Licastro, Danilo
Lovatti, Luca
Zavan, Barbara
author_sort Trentini, Martina
collection PubMed
description The constant dialogue between the plant world and the animal world (including man among them) has been known since the time of Adam and Eve, where an apple was the origin of the evils of the world. Apart from Snow White—who might have something to object to when it comes to the use of apples—fruits, plants, and natural extracts have been known for millennia as remedies for human health-related ailments. In the light of such evidence, the aim of the present work was to investigate from a biological point of view the potential role of apple exosomes in inflammatory processes on human cells. To this end we isolated and characterized apple exosomes and treated human cells such as macrophages and NCTC L929 as cancer cells in order to evaluate the tumorigenic and anti-inflammatory effect of apple exomes. Microscopic and molecular biology analyses were conducted to characterize exosomes and to assess cell proliferation, death, and miRNA line, as well as gene expression and the uptake of exosomes by cells. The results confirm the absolute biological safety of exosomes and their anti-inflammatory effect, mediated mainly by miRNA146 production by M2 macrophages.
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spelling pubmed-89624042022-03-30 An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples Trentini, Martina Zanotti, Federica Tiengo, Elena Camponogara, Francesca Degasperi, Margherita Licastro, Danilo Lovatti, Luca Zavan, Barbara Biomedicines Article The constant dialogue between the plant world and the animal world (including man among them) has been known since the time of Adam and Eve, where an apple was the origin of the evils of the world. Apart from Snow White—who might have something to object to when it comes to the use of apples—fruits, plants, and natural extracts have been known for millennia as remedies for human health-related ailments. In the light of such evidence, the aim of the present work was to investigate from a biological point of view the potential role of apple exosomes in inflammatory processes on human cells. To this end we isolated and characterized apple exosomes and treated human cells such as macrophages and NCTC L929 as cancer cells in order to evaluate the tumorigenic and anti-inflammatory effect of apple exomes. Microscopic and molecular biology analyses were conducted to characterize exosomes and to assess cell proliferation, death, and miRNA line, as well as gene expression and the uptake of exosomes by cells. The results confirm the absolute biological safety of exosomes and their anti-inflammatory effect, mediated mainly by miRNA146 production by M2 macrophages. MDPI 2022-02-10 /pmc/articles/PMC8962404/ /pubmed/35203624 http://dx.doi.org/10.3390/biomedicines10020415 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trentini, Martina
Zanotti, Federica
Tiengo, Elena
Camponogara, Francesca
Degasperi, Margherita
Licastro, Danilo
Lovatti, Luca
Zavan, Barbara
An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples
title An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples
title_full An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples
title_fullStr An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples
title_full_unstemmed An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples
title_short An Apple a Day Keeps the Doctor Away: Potential Role of miRNA 146 on Macrophages Treated with Exosomes Derived from Apples
title_sort apple a day keeps the doctor away: potential role of mirna 146 on macrophages treated with exosomes derived from apples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962404/
https://www.ncbi.nlm.nih.gov/pubmed/35203624
http://dx.doi.org/10.3390/biomedicines10020415
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