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Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope

The gold standard test for identifying SARS-CoV-2, the causative agent of COVID-19, is polymerase chain reaction (PCR). Despite their limited sensitivity, SARS-CoV-2 antigen rapid diagnostic tests are vital tools in the fight against viral spread. Owing to its simplicity and low cost, the lateral fl...

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Autores principales: Kawasaki, Hideya, Suzuki, Hiromi, Furuhashi, Kazuki, Yamashita, Keita, Ishikawa, Jinko, Nagura, Osanori, Maekawa, Masato, Miwa, Takafumi, Tandou, Takumi, Hariyama, Takahiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962435/
https://www.ncbi.nlm.nih.gov/pubmed/35203656
http://dx.doi.org/10.3390/biomedicines10020447
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author Kawasaki, Hideya
Suzuki, Hiromi
Furuhashi, Kazuki
Yamashita, Keita
Ishikawa, Jinko
Nagura, Osanori
Maekawa, Masato
Miwa, Takafumi
Tandou, Takumi
Hariyama, Takahiko
author_facet Kawasaki, Hideya
Suzuki, Hiromi
Furuhashi, Kazuki
Yamashita, Keita
Ishikawa, Jinko
Nagura, Osanori
Maekawa, Masato
Miwa, Takafumi
Tandou, Takumi
Hariyama, Takahiko
author_sort Kawasaki, Hideya
collection PubMed
description The gold standard test for identifying SARS-CoV-2, the causative agent of COVID-19, is polymerase chain reaction (PCR). Despite their limited sensitivity, SARS-CoV-2 antigen rapid diagnostic tests are vital tools in the fight against viral spread. Owing to its simplicity and low cost, the lateral flow assay (LFA) is the most extensively used point-of-care diagnostic test. Here, we report a newly designed LFA-NanoSuit method (LNSM) that works in conjunction with desktop scanning electron microscopy (SEM) to detect SARS-CoV-2. LNSM requires no standard SEM treatment, avoids cellulose and residual buffer deformation, and enables the capture of high-resolution images of antibody-labeled gold/platinum particles reacting with SARS-CoV-2 antigens. To assess its applicability, we compared clinical SARS-CoV-2 samples via visual detection of LFA, LSNM detection of LFA, and real-time reverse transcription-PCR (qRT-PCR). Compared to qRT-PCR, LNSM showed 86.7% sensitivity (26/30; 95% confidence interval (CI): 69.28–96.24%) and 93.3% specificity (14/15; 95% CI: 68.05–99.83%) for SARS-CoV-2. In samples with a relatively low SARS-CoV-2 RNA copy number (30 < Ct ≤ 40), the sensitivity of LNSM was greater (73.3%) than that of visual detection (0%). A simple, sensitive, and quantitative LNSM can be used to diagnose SARS-CoV-2.
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spelling pubmed-89624352022-03-30 Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope Kawasaki, Hideya Suzuki, Hiromi Furuhashi, Kazuki Yamashita, Keita Ishikawa, Jinko Nagura, Osanori Maekawa, Masato Miwa, Takafumi Tandou, Takumi Hariyama, Takahiko Biomedicines Article The gold standard test for identifying SARS-CoV-2, the causative agent of COVID-19, is polymerase chain reaction (PCR). Despite their limited sensitivity, SARS-CoV-2 antigen rapid diagnostic tests are vital tools in the fight against viral spread. Owing to its simplicity and low cost, the lateral flow assay (LFA) is the most extensively used point-of-care diagnostic test. Here, we report a newly designed LFA-NanoSuit method (LNSM) that works in conjunction with desktop scanning electron microscopy (SEM) to detect SARS-CoV-2. LNSM requires no standard SEM treatment, avoids cellulose and residual buffer deformation, and enables the capture of high-resolution images of antibody-labeled gold/platinum particles reacting with SARS-CoV-2 antigens. To assess its applicability, we compared clinical SARS-CoV-2 samples via visual detection of LFA, LSNM detection of LFA, and real-time reverse transcription-PCR (qRT-PCR). Compared to qRT-PCR, LNSM showed 86.7% sensitivity (26/30; 95% confidence interval (CI): 69.28–96.24%) and 93.3% specificity (14/15; 95% CI: 68.05–99.83%) for SARS-CoV-2. In samples with a relatively low SARS-CoV-2 RNA copy number (30 < Ct ≤ 40), the sensitivity of LNSM was greater (73.3%) than that of visual detection (0%). A simple, sensitive, and quantitative LNSM can be used to diagnose SARS-CoV-2. MDPI 2022-02-15 /pmc/articles/PMC8962435/ /pubmed/35203656 http://dx.doi.org/10.3390/biomedicines10020447 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kawasaki, Hideya
Suzuki, Hiromi
Furuhashi, Kazuki
Yamashita, Keita
Ishikawa, Jinko
Nagura, Osanori
Maekawa, Masato
Miwa, Takafumi
Tandou, Takumi
Hariyama, Takahiko
Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope
title Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope
title_full Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope
title_fullStr Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope
title_full_unstemmed Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope
title_short Highly Sensitive and Quantitative Diagnosis of SARS-CoV-2 Using a Gold/Platinum Particle-Based Lateral Flow Assay and a Desktop Scanning Electron Microscope
title_sort highly sensitive and quantitative diagnosis of sars-cov-2 using a gold/platinum particle-based lateral flow assay and a desktop scanning electron microscope
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962435/
https://www.ncbi.nlm.nih.gov/pubmed/35203656
http://dx.doi.org/10.3390/biomedicines10020447
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