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Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada

Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008–2009, 2013–2014, and 2017–2018. We evaluated single-nuc...

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Autores principales: Kariyawasam, Ruwandi, Lau, Rachel, Shao, Eric, Tan, Katherine, Showler, Adrienne, Ralevski, Filip, Patel, Samir N., Boggild, Andrea K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962893/
https://www.ncbi.nlm.nih.gov/pubmed/35318914
http://dx.doi.org/10.3201/eid2804.210533
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author Kariyawasam, Ruwandi
Lau, Rachel
Shao, Eric
Tan, Katherine
Showler, Adrienne
Ralevski, Filip
Patel, Samir N.
Boggild, Andrea K.
author_facet Kariyawasam, Ruwandi
Lau, Rachel
Shao, Eric
Tan, Katherine
Showler, Adrienne
Ralevski, Filip
Patel, Samir N.
Boggild, Andrea K.
author_sort Kariyawasam, Ruwandi
collection PubMed
description Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008–2009, 2013–2014, and 2017–2018. We evaluated single-nucleotide polymorphisms at atpase6 (pfATPase6), pfcrt (chloroquine resistance transporter), cytb (cytochrome b), dhfr (dihydrofolate reductase), dhps (dihydropteroate synthetase), mdr1 (multidrug resistance protein) and mdr1 copy number, and kelch13 (kelch protein gene on chromosome 13). Over time, we observed increasing mutant genotypes for dhfr S108N and dhps A613T and decreasing mutant genotypes for mdr1 N86Y, D1246Y, pfcrt K76T, and pfcrt 74–75; we identified no kelch13 mutations. We observed fewer mutations indicative of chloroquine resistance over time, which may reflect reduced chloroquine pressure in specimens from travelers to Africa. Mutations conferring proguanil resistance increased over time. Minor genotypes confirm the heterogeneous nature of infection and may affect treatment success.
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spelling pubmed-89628932022-04-02 Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada Kariyawasam, Ruwandi Lau, Rachel Shao, Eric Tan, Katherine Showler, Adrienne Ralevski, Filip Patel, Samir N. Boggild, Andrea K. Emerg Infect Dis Research Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008–2009, 2013–2014, and 2017–2018. We evaluated single-nucleotide polymorphisms at atpase6 (pfATPase6), pfcrt (chloroquine resistance transporter), cytb (cytochrome b), dhfr (dihydrofolate reductase), dhps (dihydropteroate synthetase), mdr1 (multidrug resistance protein) and mdr1 copy number, and kelch13 (kelch protein gene on chromosome 13). Over time, we observed increasing mutant genotypes for dhfr S108N and dhps A613T and decreasing mutant genotypes for mdr1 N86Y, D1246Y, pfcrt K76T, and pfcrt 74–75; we identified no kelch13 mutations. We observed fewer mutations indicative of chloroquine resistance over time, which may reflect reduced chloroquine pressure in specimens from travelers to Africa. Mutations conferring proguanil resistance increased over time. Minor genotypes confirm the heterogeneous nature of infection and may affect treatment success. Centers for Disease Control and Prevention 2022-04 /pmc/articles/PMC8962893/ /pubmed/35318914 http://dx.doi.org/10.3201/eid2804.210533 Text en https://creativecommons.org/licenses/by/4.0/Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Kariyawasam, Ruwandi
Lau, Rachel
Shao, Eric
Tan, Katherine
Showler, Adrienne
Ralevski, Filip
Patel, Samir N.
Boggild, Andrea K.
Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada
title Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada
title_full Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada
title_fullStr Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada
title_full_unstemmed Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada
title_short Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada
title_sort molecular surveillance for imported antimicrobial resistant plasmodium falciparum, ontario, canada
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962893/
https://www.ncbi.nlm.nih.gov/pubmed/35318914
http://dx.doi.org/10.3201/eid2804.210533
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