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Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada
Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008–2009, 2013–2014, and 2017–2018. We evaluated single-nuc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962893/ https://www.ncbi.nlm.nih.gov/pubmed/35318914 http://dx.doi.org/10.3201/eid2804.210533 |
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author | Kariyawasam, Ruwandi Lau, Rachel Shao, Eric Tan, Katherine Showler, Adrienne Ralevski, Filip Patel, Samir N. Boggild, Andrea K. |
author_facet | Kariyawasam, Ruwandi Lau, Rachel Shao, Eric Tan, Katherine Showler, Adrienne Ralevski, Filip Patel, Samir N. Boggild, Andrea K. |
author_sort | Kariyawasam, Ruwandi |
collection | PubMed |
description | Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008–2009, 2013–2014, and 2017–2018. We evaluated single-nucleotide polymorphisms at atpase6 (pfATPase6), pfcrt (chloroquine resistance transporter), cytb (cytochrome b), dhfr (dihydrofolate reductase), dhps (dihydropteroate synthetase), mdr1 (multidrug resistance protein) and mdr1 copy number, and kelch13 (kelch protein gene on chromosome 13). Over time, we observed increasing mutant genotypes for dhfr S108N and dhps A613T and decreasing mutant genotypes for mdr1 N86Y, D1246Y, pfcrt K76T, and pfcrt 74–75; we identified no kelch13 mutations. We observed fewer mutations indicative of chloroquine resistance over time, which may reflect reduced chloroquine pressure in specimens from travelers to Africa. Mutations conferring proguanil resistance increased over time. Minor genotypes confirm the heterogeneous nature of infection and may affect treatment success. |
format | Online Article Text |
id | pubmed-8962893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-89628932022-04-02 Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada Kariyawasam, Ruwandi Lau, Rachel Shao, Eric Tan, Katherine Showler, Adrienne Ralevski, Filip Patel, Samir N. Boggild, Andrea K. Emerg Infect Dis Research Single-nucleotide polymorphisms at several loci have been correlated with Plasmodium falciparum drug resistance. We examined the prevalence of resistance markers in P. falciparum from imported malaria cases in Canada during 3 time periods, 2008–2009, 2013–2014, and 2017–2018. We evaluated single-nucleotide polymorphisms at atpase6 (pfATPase6), pfcrt (chloroquine resistance transporter), cytb (cytochrome b), dhfr (dihydrofolate reductase), dhps (dihydropteroate synthetase), mdr1 (multidrug resistance protein) and mdr1 copy number, and kelch13 (kelch protein gene on chromosome 13). Over time, we observed increasing mutant genotypes for dhfr S108N and dhps A613T and decreasing mutant genotypes for mdr1 N86Y, D1246Y, pfcrt K76T, and pfcrt 74–75; we identified no kelch13 mutations. We observed fewer mutations indicative of chloroquine resistance over time, which may reflect reduced chloroquine pressure in specimens from travelers to Africa. Mutations conferring proguanil resistance increased over time. Minor genotypes confirm the heterogeneous nature of infection and may affect treatment success. Centers for Disease Control and Prevention 2022-04 /pmc/articles/PMC8962893/ /pubmed/35318914 http://dx.doi.org/10.3201/eid2804.210533 Text en https://creativecommons.org/licenses/by/4.0/Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Kariyawasam, Ruwandi Lau, Rachel Shao, Eric Tan, Katherine Showler, Adrienne Ralevski, Filip Patel, Samir N. Boggild, Andrea K. Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada |
title | Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada |
title_full | Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada |
title_fullStr | Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada |
title_full_unstemmed | Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada |
title_short | Molecular Surveillance for Imported Antimicrobial Resistant Plasmodium falciparum, Ontario, Canada |
title_sort | molecular surveillance for imported antimicrobial resistant plasmodium falciparum, ontario, canada |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962893/ https://www.ncbi.nlm.nih.gov/pubmed/35318914 http://dx.doi.org/10.3201/eid2804.210533 |
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