Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA

Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation wi...

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Detalles Bibliográficos
Autores principales: Heguy, Adriana, Dimartino, Dacia, Marier, Christian, Zappile, Paul, Guzman, Emily, Duerr, Ralf, Wang, Guiqing, Plitnick, Jonathan, Russell, Alexis, Lamson, Daryl M., St. George, Kirsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2022
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962901/
https://www.ncbi.nlm.nih.gov/pubmed/35130474
http://dx.doi.org/10.3201/eid2804.220146
Descripción
Sumario:Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution.

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