Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA

Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation wi...

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Detalles Bibliográficos
Autores principales: Heguy, Adriana, Dimartino, Dacia, Marier, Christian, Zappile, Paul, Guzman, Emily, Duerr, Ralf, Wang, Guiqing, Plitnick, Jonathan, Russell, Alexis, Lamson, Daryl M., St. George, Kirsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2022
Materias:
Research Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962901/
https://www.ncbi.nlm.nih.gov/pubmed/35130474
http://dx.doi.org/10.3201/eid2804.220146
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author Heguy, Adriana
Dimartino, Dacia
Marier, Christian
Zappile, Paul
Guzman, Emily
Duerr, Ralf
Wang, Guiqing
Plitnick, Jonathan
Russell, Alexis
Lamson, Daryl M.
St. George, Kirsten
author_facet Heguy, Adriana
Dimartino, Dacia
Marier, Christian
Zappile, Paul
Guzman, Emily
Duerr, Ralf
Wang, Guiqing
Plitnick, Jonathan
Russell, Alexis
Lamson, Daryl M.
St. George, Kirsten
author_sort Heguy, Adriana
collection PubMed
description Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution.
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spelling pubmed-89629012022-04-02 Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA Heguy, Adriana Dimartino, Dacia Marier, Christian Zappile, Paul Guzman, Emily Duerr, Ralf Wang, Guiqing Plitnick, Jonathan Russell, Alexis Lamson, Daryl M. St. George, Kirsten Emerg Infect Dis Research Letter Of 379 severe acute respiratory syndrome coronavirus 2 samples collected in New York, USA, we detected 86 Omicron variant sequences containing Delta variant mutation P681R. Probable explanations were co-infection with 2 viruses or contamination/amplification artifact. Repeated library preparation with fewer cycles showed the P681R calls were artifactual. Unusual mutations should be interpreted with caution. Centers for Disease Control and Prevention 2022-04 /pmc/articles/PMC8962901/ /pubmed/35130474 http://dx.doi.org/10.3201/eid2804.220146 Text en https://creativecommons.org/licenses/by/4.0/Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research Letter
Heguy, Adriana
Dimartino, Dacia
Marier, Christian
Zappile, Paul
Guzman, Emily
Duerr, Ralf
Wang, Guiqing
Plitnick, Jonathan
Russell, Alexis
Lamson, Daryl M.
St. George, Kirsten
Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
title Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
title_full Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
title_fullStr Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
title_full_unstemmed Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
title_short Amplification Artifact in SARS-CoV-2 Omicron Sequences Carrying P681R Mutation, New York, USA
title_sort amplification artifact in sars-cov-2 omicron sequences carrying p681r mutation, new york, usa
topic Research Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8962901/
https://www.ncbi.nlm.nih.gov/pubmed/35130474
http://dx.doi.org/10.3201/eid2804.220146
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