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Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties

The field of regenerative medicine has recently seen an emerging trend toward decellularized extracellular matrix (ECM) as a biological scaffold for stem cell-delivery. Human umbilical cord represents a valuable opportunity from both technical and ethical point of view to obtain allogenic ECM. Herei...

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Autores principales: Dubus, M., Scomazzon, L., Chevrier, J., Montanede, A., Baldit, A., Terryn, C., Quilès, F., Thomachot-Schneider, C., Gangloff, S. C., Bouland, N., Gindraux, F., Rammal, H., Mauprivez, C., Kerdjoudj, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963334/
https://www.ncbi.nlm.nih.gov/pubmed/35360386
http://dx.doi.org/10.3389/fbioe.2022.828424
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author Dubus, M.
Scomazzon, L.
Chevrier, J.
Montanede, A.
Baldit, A.
Terryn, C.
Quilès, F.
Thomachot-Schneider, C.
Gangloff, S. C.
Bouland, N.
Gindraux, F.
Rammal, H.
Mauprivez, C.
Kerdjoudj, H.
author_facet Dubus, M.
Scomazzon, L.
Chevrier, J.
Montanede, A.
Baldit, A.
Terryn, C.
Quilès, F.
Thomachot-Schneider, C.
Gangloff, S. C.
Bouland, N.
Gindraux, F.
Rammal, H.
Mauprivez, C.
Kerdjoudj, H.
author_sort Dubus, M.
collection PubMed
description The field of regenerative medicine has recently seen an emerging trend toward decellularized extracellular matrix (ECM) as a biological scaffold for stem cell-delivery. Human umbilical cord represents a valuable opportunity from both technical and ethical point of view to obtain allogenic ECM. Herein, we established a protocol, allowing the full removal of cell membranes and nuclei moieties from Wharton’s jelly (WJ) tissue. No alterations in the ECM components (i.e., collagen, GAG content, and growth factors), physical (i.e., porosity and swelling) and mechanical (i.e., linear tensile modulus) properties were noticed following WJ processing. Furthermore, no effect of the tissue processing on macromolecules and growth factors retention was observed, assuring thus a suitable bioactive matrix for cell maintenance upon recellularization. Based on the in vitro and in vivo biodegradability and stromal cell homing capabilities, decellularized WJ could provide an ideal substrate for stromal cells adhesion and colonization. Interestingly, the tissue processing increased the antibacterial and antiadhesive properties of WJ against Staphylococcus aureus and Staphylococcus epidermidis pathogens. Altogether, our results indicate that decellularized WJ matrix is able to limit Staphylococcus-related infections and to promote stromal cell homing, thus offering a versatile scaffold for tissue regenerative medicine.
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spelling pubmed-89633342022-03-30 Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties Dubus, M. Scomazzon, L. Chevrier, J. Montanede, A. Baldit, A. Terryn, C. Quilès, F. Thomachot-Schneider, C. Gangloff, S. C. Bouland, N. Gindraux, F. Rammal, H. Mauprivez, C. Kerdjoudj, H. Front Bioeng Biotechnol Bioengineering and Biotechnology The field of regenerative medicine has recently seen an emerging trend toward decellularized extracellular matrix (ECM) as a biological scaffold for stem cell-delivery. Human umbilical cord represents a valuable opportunity from both technical and ethical point of view to obtain allogenic ECM. Herein, we established a protocol, allowing the full removal of cell membranes and nuclei moieties from Wharton’s jelly (WJ) tissue. No alterations in the ECM components (i.e., collagen, GAG content, and growth factors), physical (i.e., porosity and swelling) and mechanical (i.e., linear tensile modulus) properties were noticed following WJ processing. Furthermore, no effect of the tissue processing on macromolecules and growth factors retention was observed, assuring thus a suitable bioactive matrix for cell maintenance upon recellularization. Based on the in vitro and in vivo biodegradability and stromal cell homing capabilities, decellularized WJ could provide an ideal substrate for stromal cells adhesion and colonization. Interestingly, the tissue processing increased the antibacterial and antiadhesive properties of WJ against Staphylococcus aureus and Staphylococcus epidermidis pathogens. Altogether, our results indicate that decellularized WJ matrix is able to limit Staphylococcus-related infections and to promote stromal cell homing, thus offering a versatile scaffold for tissue regenerative medicine. Frontiers Media S.A. 2022-03-11 /pmc/articles/PMC8963334/ /pubmed/35360386 http://dx.doi.org/10.3389/fbioe.2022.828424 Text en Copyright © 2022 Dubus, Scomazzon, Chevrier, Montanede, Baldit, Terryn, Quilès, Thomachot-Schneider, Gangloff, Bouland, Gindraux, Rammal, Mauprivez and Kerdjoudj. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Dubus, M.
Scomazzon, L.
Chevrier, J.
Montanede, A.
Baldit, A.
Terryn, C.
Quilès, F.
Thomachot-Schneider, C.
Gangloff, S. C.
Bouland, N.
Gindraux, F.
Rammal, H.
Mauprivez, C.
Kerdjoudj, H.
Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties
title Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties
title_full Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties
title_fullStr Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties
title_full_unstemmed Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties
title_short Decellularization of Wharton’s Jelly Increases Its Bioactivity and Antibacterial Properties
title_sort decellularization of wharton’s jelly increases its bioactivity and antibacterial properties
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963334/
https://www.ncbi.nlm.nih.gov/pubmed/35360386
http://dx.doi.org/10.3389/fbioe.2022.828424
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