Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model

Testicular development is imperative to spermatogenesis, and pre-puberty is the key period for testis development. This study, therefore, investigated the effects of fibre supplementation on testis development and its possible mechanism in a growing boar model. Thirty Yorkshire boars were randomly d...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Yan, Wu, De, Che, Lianqiang, Fang, Zhengfeng, Xu, Shengyu, Feng, Bin, Zhuo, Yong, Li, Jian, Wu, Caimei, Zhang, Junjie, Li, Lujie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Veterinary Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963373/
https://www.ncbi.nlm.nih.gov/pubmed/35359689
http://dx.doi.org/10.3389/fvets.2022.850685
_version_ 1784677976550408192
author Lin, Yan
Wu, De
Che, Lianqiang
Fang, Zhengfeng
Xu, Shengyu
Feng, Bin
Zhuo, Yong
Li, Jian
Wu, Caimei
Zhang, Junjie
Li, Lujie
author_facet Lin, Yan
Wu, De
Che, Lianqiang
Fang, Zhengfeng
Xu, Shengyu
Feng, Bin
Zhuo, Yong
Li, Jian
Wu, Caimei
Zhang, Junjie
Li, Lujie
author_sort Lin, Yan
collection PubMed
description Testicular development is imperative to spermatogenesis, and pre-puberty is the key period for testis development. This study, therefore, investigated the effects of fibre supplementation on testis development and its possible mechanism in a growing boar model. Thirty Yorkshire boars were randomly divided into a control group (Control) and a fibre group (Fibre) from day 0 to 90 after weaning, with three pigs per pen and five pens per treatment. Blood and testes were collected for analysis. Dietary fibre supplementation had no significant effect on growth performance, testicular volume, or libido but increased the semen production of boars. Boars fed with fibre had lower serum cholesterol (CHO) and low-density lipoprotein (LDL) levels compared to those on the Control diet; however, testicular CHO, triglyceride (TG), and LDL concentration in the Fibre group were significantly higher than the Control group (P < 0.01). Testicular histological analysis showed that seminiferous tubules and testicular germ cells of 120-day-old boars were densely arranged in the Fibre group, and the number of Leydig cells was significantly higher than that of the Control group (P < 0.001). Furthermore, the diet supplemented with fibre significantly decreased leptin, leptin receptor (Leptor), and luteinising hormone (LH) concentrations in boar serum (P < 0.05), whereas follicle-stimulating hormone (FSH) and testosterone concentrations were significantly increased (P < 0.05). Meanwhile, the expression of AMH, AMHR2, and SYCP3 genes related to proliferation and differentiation, and hormone-related genes STAR and SOCS3, were significantly up-regulated (P < 0.05). OCCLUDIN expression was up-regulated, whereas CDH2 expression was down-regulated. In conclusion, increased fibre intake during the pre-puberty period in growing boar is crucial for Leydig cell proliferation, up-regulating the expression of genes related to hormone synthesis and thereby promoting the secretion of testosterone and semen production.
format Online
Article
Text
id pubmed-8963373
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-89633732022-03-30 Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model Lin, Yan Wu, De Che, Lianqiang Fang, Zhengfeng Xu, Shengyu Feng, Bin Zhuo, Yong Li, Jian Wu, Caimei Zhang, Junjie Li, Lujie Front Vet Sci Veterinary Science Testicular development is imperative to spermatogenesis, and pre-puberty is the key period for testis development. This study, therefore, investigated the effects of fibre supplementation on testis development and its possible mechanism in a growing boar model. Thirty Yorkshire boars were randomly divided into a control group (Control) and a fibre group (Fibre) from day 0 to 90 after weaning, with three pigs per pen and five pens per treatment. Blood and testes were collected for analysis. Dietary fibre supplementation had no significant effect on growth performance, testicular volume, or libido but increased the semen production of boars. Boars fed with fibre had lower serum cholesterol (CHO) and low-density lipoprotein (LDL) levels compared to those on the Control diet; however, testicular CHO, triglyceride (TG), and LDL concentration in the Fibre group were significantly higher than the Control group (P < 0.01). Testicular histological analysis showed that seminiferous tubules and testicular germ cells of 120-day-old boars were densely arranged in the Fibre group, and the number of Leydig cells was significantly higher than that of the Control group (P < 0.001). Furthermore, the diet supplemented with fibre significantly decreased leptin, leptin receptor (Leptor), and luteinising hormone (LH) concentrations in boar serum (P < 0.05), whereas follicle-stimulating hormone (FSH) and testosterone concentrations were significantly increased (P < 0.05). Meanwhile, the expression of AMH, AMHR2, and SYCP3 genes related to proliferation and differentiation, and hormone-related genes STAR and SOCS3, were significantly up-regulated (P < 0.05). OCCLUDIN expression was up-regulated, whereas CDH2 expression was down-regulated. In conclusion, increased fibre intake during the pre-puberty period in growing boar is crucial for Leydig cell proliferation, up-regulating the expression of genes related to hormone synthesis and thereby promoting the secretion of testosterone and semen production. Frontiers Media S.A. 2022-03-11 /pmc/articles/PMC8963373/ /pubmed/35359689 http://dx.doi.org/10.3389/fvets.2022.850685 Text en Copyright © 2022 Lin, Wu, Che, Fang, Xu, Feng, Zhuo, Li, Wu, Zhang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Lin, Yan
Wu, De
Che, Lianqiang
Fang, Zhengfeng
Xu, Shengyu
Feng, Bin
Zhuo, Yong
Li, Jian
Wu, Caimei
Zhang, Junjie
Li, Lujie
Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model
title Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model
title_full Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model
title_fullStr Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model
title_full_unstemmed Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model
title_short Dietary Fibre Supplementation Improves Semen Production by Increasing Leydig Cells and Testosterone Synthesis in a Growing Boar Model
title_sort dietary fibre supplementation improves semen production by increasing leydig cells and testosterone synthesis in a growing boar model
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963373/
https://www.ncbi.nlm.nih.gov/pubmed/35359689
http://dx.doi.org/10.3389/fvets.2022.850685
work_keys_str_mv AT linyan dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT wude dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT chelianqiang dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT fangzhengfeng dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT xushengyu dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT fengbin dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT zhuoyong dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT lijian dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT wucaimei dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT zhangjunjie dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel
AT lilujie dietaryfibresupplementationimprovessemenproductionbyincreasingleydigcellsandtestosteronesynthesisinagrowingboarmodel

Ejemplares similares