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Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate

PURPOSE: To study the dimensions and distribution of human vitreous collagen type II fragments collected after vitrectomy performed at varying cut rates and to evaluate if increasing the cut rate produces smaller collagen fragments, thus reducing retinal traction and/or viscosity. METHODS: Fluid was...

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Autores principales: Rossi, Tommaso, Speciale, Andrea, Menichini, Paola, Izzotti, Alberto, D'Agostino, Isabella, Trillo, Carlandrea, Telani, Serena, Querzoli, Giorgio, Ripandelli, Guido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963665/
https://www.ncbi.nlm.nih.gov/pubmed/35333285
http://dx.doi.org/10.1167/tvst.11.3.29
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author Rossi, Tommaso
Speciale, Andrea
Menichini, Paola
Izzotti, Alberto
D'Agostino, Isabella
Trillo, Carlandrea
Telani, Serena
Querzoli, Giorgio
Ripandelli, Guido
author_facet Rossi, Tommaso
Speciale, Andrea
Menichini, Paola
Izzotti, Alberto
D'Agostino, Isabella
Trillo, Carlandrea
Telani, Serena
Querzoli, Giorgio
Ripandelli, Guido
author_sort Rossi, Tommaso
collection PubMed
description PURPOSE: To study the dimensions and distribution of human vitreous collagen type II fragments collected after vitrectomy performed at varying cut rates and to evaluate if increasing the cut rate produces smaller collagen fragments, thus reducing retinal traction and/or viscosity. METHODS: Fluid was collected during core vitrectomies performed for macular surgery at cut rates from 1000 to 16,000 cuts per minute (CPM) and immediately refrigerated. Protein fractions were separated by molecular weight (MW; >100 kDa, 50–100 kDa, 50–30 kDa, 30–10 kDa, and <10 kDa) through centrifugal filters. The Human Collagen II ELISA Kit colorimetric assay was then used to measure the COL2A1 in unfiltered and filtered samples. RESULTS: Vitreous samples collected after vitrectomy performed at 16,000 CPM contained a higher concentration of protein with MW over 100 kDa than at any other cutting frequency (P < 0.01). No significant differences were found in fractions collected with a MW between 50 and 100 kDa. Collagen type II fragments over 100 kDa were significantly more represented than smaller fragments at each cut rate. The proportion of smaller (50–100 kDa) collagen fragments compared with those over 100 kDa was higher at 2000 CPM than at higher cut rates. CONCLUSIONS: Vitreous samples collected at different cut rates do not contain a significantly different proportion of collagen type II fragments of the tested MW. The extreme variability of vitreous flow through the cutter port may explain the uncertain predictability of collagen fragment MWs. TRANSLATIONAL RELEVANCE: Increasing the cut rate does not produce vitreous fragments of proportionally smaller dimension. It is necessary to achieve an invariant instantaneous flow through the cutter port in order to decrease retinal traction during vitrectomy.
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spelling pubmed-89636652022-03-30 Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate Rossi, Tommaso Speciale, Andrea Menichini, Paola Izzotti, Alberto D'Agostino, Isabella Trillo, Carlandrea Telani, Serena Querzoli, Giorgio Ripandelli, Guido Transl Vis Sci Technol Article PURPOSE: To study the dimensions and distribution of human vitreous collagen type II fragments collected after vitrectomy performed at varying cut rates and to evaluate if increasing the cut rate produces smaller collagen fragments, thus reducing retinal traction and/or viscosity. METHODS: Fluid was collected during core vitrectomies performed for macular surgery at cut rates from 1000 to 16,000 cuts per minute (CPM) and immediately refrigerated. Protein fractions were separated by molecular weight (MW; >100 kDa, 50–100 kDa, 50–30 kDa, 30–10 kDa, and <10 kDa) through centrifugal filters. The Human Collagen II ELISA Kit colorimetric assay was then used to measure the COL2A1 in unfiltered and filtered samples. RESULTS: Vitreous samples collected after vitrectomy performed at 16,000 CPM contained a higher concentration of protein with MW over 100 kDa than at any other cutting frequency (P < 0.01). No significant differences were found in fractions collected with a MW between 50 and 100 kDa. Collagen type II fragments over 100 kDa were significantly more represented than smaller fragments at each cut rate. The proportion of smaller (50–100 kDa) collagen fragments compared with those over 100 kDa was higher at 2000 CPM than at higher cut rates. CONCLUSIONS: Vitreous samples collected at different cut rates do not contain a significantly different proportion of collagen type II fragments of the tested MW. The extreme variability of vitreous flow through the cutter port may explain the uncertain predictability of collagen fragment MWs. TRANSLATIONAL RELEVANCE: Increasing the cut rate does not produce vitreous fragments of proportionally smaller dimension. It is necessary to achieve an invariant instantaneous flow through the cutter port in order to decrease retinal traction during vitrectomy. The Association for Research in Vision and Ophthalmology 2022-03-25 /pmc/articles/PMC8963665/ /pubmed/35333285 http://dx.doi.org/10.1167/tvst.11.3.29 Text en Copyright 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Article
Rossi, Tommaso
Speciale, Andrea
Menichini, Paola
Izzotti, Alberto
D'Agostino, Isabella
Trillo, Carlandrea
Telani, Serena
Querzoli, Giorgio
Ripandelli, Guido
Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate
title Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate
title_full Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate
title_fullStr Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate
title_full_unstemmed Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate
title_short Human Vitreous Collagen Fragments Dimension As a Function of Vitrectomy Cut Rate
title_sort human vitreous collagen fragments dimension as a function of vitrectomy cut rate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963665/
https://www.ncbi.nlm.nih.gov/pubmed/35333285
http://dx.doi.org/10.1167/tvst.11.3.29
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