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From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro
Advances in the field of stem cell-based models have in recent years lead to the development of blastocyst-like structures termed blastoids. Blastoids can be used to study key events in mammalian pre-implantation development, as they mimic the blastocyst morphologically and transcriptionally, can pr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963787/ https://www.ncbi.nlm.nih.gov/pubmed/35359453 http://dx.doi.org/10.3389/fcell.2022.838356 |
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author | Luijkx, Dorian Shankar, Vinidhra van Blitterswijk, Clemens Giselbrecht, Stefan Vrij, Erik |
author_facet | Luijkx, Dorian Shankar, Vinidhra van Blitterswijk, Clemens Giselbrecht, Stefan Vrij, Erik |
author_sort | Luijkx, Dorian |
collection | PubMed |
description | Advances in the field of stem cell-based models have in recent years lead to the development of blastocyst-like structures termed blastoids. Blastoids can be used to study key events in mammalian pre-implantation development, as they mimic the blastocyst morphologically and transcriptionally, can progress to the post-implantation stage and can be generated in large numbers. Blastoids were originally developed using mouse pluripotent stem cells, and since several groups have successfully generated blastocyst models of the human system. Here we provide a comparison of the mouse and human protocols with the aim of deriving the core requirements for blastoid formation, discuss the models’ current ability to mimic blastocysts and give an outlook on potential future applications. |
format | Online Article Text |
id | pubmed-8963787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-89637872022-03-30 From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro Luijkx, Dorian Shankar, Vinidhra van Blitterswijk, Clemens Giselbrecht, Stefan Vrij, Erik Front Cell Dev Biol Cell and Developmental Biology Advances in the field of stem cell-based models have in recent years lead to the development of blastocyst-like structures termed blastoids. Blastoids can be used to study key events in mammalian pre-implantation development, as they mimic the blastocyst morphologically and transcriptionally, can progress to the post-implantation stage and can be generated in large numbers. Blastoids were originally developed using mouse pluripotent stem cells, and since several groups have successfully generated blastocyst models of the human system. Here we provide a comparison of the mouse and human protocols with the aim of deriving the core requirements for blastoid formation, discuss the models’ current ability to mimic blastocysts and give an outlook on potential future applications. Frontiers Media S.A. 2022-03-11 /pmc/articles/PMC8963787/ /pubmed/35359453 http://dx.doi.org/10.3389/fcell.2022.838356 Text en Copyright © 2022 Luijkx, Shankar, van Blitterswijk, Giselbrecht and Vrij. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Luijkx, Dorian Shankar, Vinidhra van Blitterswijk, Clemens Giselbrecht, Stefan Vrij, Erik From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro |
title | From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro
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title_full | From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro
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title_fullStr | From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro
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title_full_unstemmed | From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro
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title_short | From Mice to Men: Generation of Human Blastocyst-Like Structures In Vitro
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title_sort | from mice to men: generation of human blastocyst-like structures in vitro |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963787/ https://www.ncbi.nlm.nih.gov/pubmed/35359453 http://dx.doi.org/10.3389/fcell.2022.838356 |
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