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Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs

Natural polymer-based films, due to their favorable biological and mechanical properties, have demonstrated great potential as coatings for biomedical applications. Among them, chitosan films have been widely studied both as coating materials and as controlled drug release systems. Crosslinkers are...

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Autores principales: Wiggers, Helton José, Chevallier, Pascale, Copes, Francesco, Simch, Fernanda Heloisa, da Silva Veloso, Felipe, Genevro, Giovana Maria, Mantovani, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963995/
https://www.ncbi.nlm.nih.gov/pubmed/35360400
http://dx.doi.org/10.3389/fbioe.2022.814162
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author Wiggers, Helton José
Chevallier, Pascale
Copes, Francesco
Simch, Fernanda Heloisa
da Silva Veloso, Felipe
Genevro, Giovana Maria
Mantovani, Diego
author_facet Wiggers, Helton José
Chevallier, Pascale
Copes, Francesco
Simch, Fernanda Heloisa
da Silva Veloso, Felipe
Genevro, Giovana Maria
Mantovani, Diego
author_sort Wiggers, Helton José
collection PubMed
description Natural polymer-based films, due to their favorable biological and mechanical properties, have demonstrated great potential as coatings for biomedical applications. Among them, chitosan films have been widely studied both as coating materials and as controlled drug release systems. Crosslinkers are often used to tune chitosan’s crosslinking degree and thus to control the drug release kinetics. For this purpose, quercetin, a plant-derived natural polyphenol, has gained attention as a crosslinker, mainly for its intrinsic anti-inflammatory, antioxidant, and antibacterial features. In this study, chitosan films crosslinked with three different concentrations of quercetin (10, 20, and 30% w/w) have been used as controlled release systems for the delivery of the antibacterial drug trimethoprim (TMP, 10% w/w). Physicochemical and antimicrobial properties were investigated. Surface wettability and composition of the films were assessed by contact angle measurements, X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared spectroscopy (FTIR), respectively. The release kinetic of TMP in phosphate-buffered saline (PBS) and 2-(N-morpholino) ethanesulfonic acid (MES) was studied over time. Finally, antibacterial properties were assessed on E. coli and S. aureus through Kirby–Bauer disc diffusion and micro-dilution broth assays. Results show that quercetin, at the tested concentrations, clearly increases the crosslinking degree in a dose-dependent manner, thus influencing the release kinetic of the loaded TMP while maintaining its bactericidal effects. In conclusion, this work demonstrates that quercetin-crosslinked chitosan films represent a promising strategy for the design of antibiotic-releasing coatings for biomedical applications.
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spelling pubmed-89639952022-03-30 Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs Wiggers, Helton José Chevallier, Pascale Copes, Francesco Simch, Fernanda Heloisa da Silva Veloso, Felipe Genevro, Giovana Maria Mantovani, Diego Front Bioeng Biotechnol Bioengineering and Biotechnology Natural polymer-based films, due to their favorable biological and mechanical properties, have demonstrated great potential as coatings for biomedical applications. Among them, chitosan films have been widely studied both as coating materials and as controlled drug release systems. Crosslinkers are often used to tune chitosan’s crosslinking degree and thus to control the drug release kinetics. For this purpose, quercetin, a plant-derived natural polyphenol, has gained attention as a crosslinker, mainly for its intrinsic anti-inflammatory, antioxidant, and antibacterial features. In this study, chitosan films crosslinked with three different concentrations of quercetin (10, 20, and 30% w/w) have been used as controlled release systems for the delivery of the antibacterial drug trimethoprim (TMP, 10% w/w). Physicochemical and antimicrobial properties were investigated. Surface wettability and composition of the films were assessed by contact angle measurements, X-ray photoelectron spectroscopy (XPS), and Fourier-transform infrared spectroscopy (FTIR), respectively. The release kinetic of TMP in phosphate-buffered saline (PBS) and 2-(N-morpholino) ethanesulfonic acid (MES) was studied over time. Finally, antibacterial properties were assessed on E. coli and S. aureus through Kirby–Bauer disc diffusion and micro-dilution broth assays. Results show that quercetin, at the tested concentrations, clearly increases the crosslinking degree in a dose-dependent manner, thus influencing the release kinetic of the loaded TMP while maintaining its bactericidal effects. In conclusion, this work demonstrates that quercetin-crosslinked chitosan films represent a promising strategy for the design of antibiotic-releasing coatings for biomedical applications. Frontiers Media S.A. 2022-03-14 /pmc/articles/PMC8963995/ /pubmed/35360400 http://dx.doi.org/10.3389/fbioe.2022.814162 Text en Copyright © 2022 Wiggers, Chevallier, Copes, Simch, da Silva Veloso, Genevro and Mantovani. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Wiggers, Helton José
Chevallier, Pascale
Copes, Francesco
Simch, Fernanda Heloisa
da Silva Veloso, Felipe
Genevro, Giovana Maria
Mantovani, Diego
Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs
title Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs
title_full Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs
title_fullStr Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs
title_full_unstemmed Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs
title_short Quercetin-Crosslinked Chitosan Films for Controlled Release of Antimicrobial Drugs
title_sort quercetin-crosslinked chitosan films for controlled release of antimicrobial drugs
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8963995/
https://www.ncbi.nlm.nih.gov/pubmed/35360400
http://dx.doi.org/10.3389/fbioe.2022.814162
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