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Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice

Preliminary clinical and basic researches have proved that Qishen granule (QSG) is an effective prescription for treating heart failure (HF) in China, with a characteristic of regulating the ratio of M1/M2 macrophage in the myocardium. However, the regulative mechanism of monocytes targeting the car...

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Autores principales: Li, Yanqin, Li, Xuan, Chen, Xu, Sun, Xiaoqian, Liu, Xiangning, Wang, Gang, Liu, Yizhou, Cui, Lingwen, Liu, Tianhua, Wang, Wei, Wang, Yong, Li, Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964526/
https://www.ncbi.nlm.nih.gov/pubmed/35370707
http://dx.doi.org/10.3389/fphar.2022.850187
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author Li, Yanqin
Li, Xuan
Chen, Xu
Sun, Xiaoqian
Liu, Xiangning
Wang, Gang
Liu, Yizhou
Cui, Lingwen
Liu, Tianhua
Wang, Wei
Wang, Yong
Li, Chun
author_facet Li, Yanqin
Li, Xuan
Chen, Xu
Sun, Xiaoqian
Liu, Xiangning
Wang, Gang
Liu, Yizhou
Cui, Lingwen
Liu, Tianhua
Wang, Wei
Wang, Yong
Li, Chun
author_sort Li, Yanqin
collection PubMed
description Preliminary clinical and basic researches have proved that Qishen granule (QSG) is an effective prescription for treating heart failure (HF) in China, with a characteristic of regulating the ratio of M1/M2 macrophage in the myocardium. However, the regulative mechanism of monocytes targeting the cardio-splenic axis has not been fully elucidated. This study aimed to investigate the effects and mechanism of QSG inhibiting the release of splenic monocytes and the recruitment of myocardial tissue both in vivo and in vitro. Experiments in mice with acute myocardial infarction (AMI)-induced HF demonstrated that QSG could exert anti-inflammatory effects by inhibiting splenic monocytes release and phenotypic changes. Moreover, in vitro experiments indicated QSG could inhibit LPS-stimulated macrophage-conditioned medium (CM)-induced H9C2 cardiomyocyte injury by upregulating the key proteins in TLR4-MyD88-NF-κB p65 pathway. In addition, knockdown or overexpression of TLR4 in H9C2 cells further confirmed that QSG could attenuate inflammatory injury in cardiomyocytes via the TLR4-MyD88-NF-κB p65 pathway. Overall, these data suggested that QSG could improve cardiac function and reduce the inflammatory response in AMI-induced HF by inhibiting splenic monocytes release, and protecting myocardial function via the TLR4-MyD88-NF-κB pathway in heart failure mice.
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spelling pubmed-89645262022-03-31 Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice Li, Yanqin Li, Xuan Chen, Xu Sun, Xiaoqian Liu, Xiangning Wang, Gang Liu, Yizhou Cui, Lingwen Liu, Tianhua Wang, Wei Wang, Yong Li, Chun Front Pharmacol Pharmacology Preliminary clinical and basic researches have proved that Qishen granule (QSG) is an effective prescription for treating heart failure (HF) in China, with a characteristic of regulating the ratio of M1/M2 macrophage in the myocardium. However, the regulative mechanism of monocytes targeting the cardio-splenic axis has not been fully elucidated. This study aimed to investigate the effects and mechanism of QSG inhibiting the release of splenic monocytes and the recruitment of myocardial tissue both in vivo and in vitro. Experiments in mice with acute myocardial infarction (AMI)-induced HF demonstrated that QSG could exert anti-inflammatory effects by inhibiting splenic monocytes release and phenotypic changes. Moreover, in vitro experiments indicated QSG could inhibit LPS-stimulated macrophage-conditioned medium (CM)-induced H9C2 cardiomyocyte injury by upregulating the key proteins in TLR4-MyD88-NF-κB p65 pathway. In addition, knockdown or overexpression of TLR4 in H9C2 cells further confirmed that QSG could attenuate inflammatory injury in cardiomyocytes via the TLR4-MyD88-NF-κB p65 pathway. Overall, these data suggested that QSG could improve cardiac function and reduce the inflammatory response in AMI-induced HF by inhibiting splenic monocytes release, and protecting myocardial function via the TLR4-MyD88-NF-κB pathway in heart failure mice. Frontiers Media S.A. 2022-03-15 /pmc/articles/PMC8964526/ /pubmed/35370707 http://dx.doi.org/10.3389/fphar.2022.850187 Text en Copyright © 2022 Li, Li, Chen, Sun, Liu, Wang, Liu, Cui, Liu, Wang, Wang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Li, Yanqin
Li, Xuan
Chen, Xu
Sun, Xiaoqian
Liu, Xiangning
Wang, Gang
Liu, Yizhou
Cui, Lingwen
Liu, Tianhua
Wang, Wei
Wang, Yong
Li, Chun
Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice
title Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice
title_full Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice
title_fullStr Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice
title_full_unstemmed Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice
title_short Qishen Granule (QSG) Inhibits Monocytes Released From the Spleen and Protect Myocardial Function via the TLR4-MyD88-NF-κB p65 Pathway in Heart Failure Mice
title_sort qishen granule (qsg) inhibits monocytes released from the spleen and protect myocardial function via the tlr4-myd88-nf-κb p65 pathway in heart failure mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964526/
https://www.ncbi.nlm.nih.gov/pubmed/35370707
http://dx.doi.org/10.3389/fphar.2022.850187
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