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Metabolomic serum abnormalities in dogs with hepatopathies

Hepatopathies can cause major metabolic abnormalities in humans and animals. This study examined differences in serum metabolomic parameters and patterns in left-over serum samples from dogs with either congenital portosystemic shunts (cPSS, n = 24) or high serum liver enzyme activities (HLEA, n = 2...

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Autores principales: Imbery, Carolin A., Dieterle, Frank, Ottka, Claudia, Weber, Corinna, Schlotterbeck, Götz, Müller, Elisabeth, Lohi, Hannes, Giger, Urs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964695/
https://www.ncbi.nlm.nih.gov/pubmed/35351920
http://dx.doi.org/10.1038/s41598-022-09056-5
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author Imbery, Carolin A.
Dieterle, Frank
Ottka, Claudia
Weber, Corinna
Schlotterbeck, Götz
Müller, Elisabeth
Lohi, Hannes
Giger, Urs
author_facet Imbery, Carolin A.
Dieterle, Frank
Ottka, Claudia
Weber, Corinna
Schlotterbeck, Götz
Müller, Elisabeth
Lohi, Hannes
Giger, Urs
author_sort Imbery, Carolin A.
collection PubMed
description Hepatopathies can cause major metabolic abnormalities in humans and animals. This study examined differences in serum metabolomic parameters and patterns in left-over serum samples from dogs with either congenital portosystemic shunts (cPSS, n = 24) or high serum liver enzyme activities (HLEA, n = 25) compared to control dogs (n = 64). A validated targeted proton nuclear magnetic resonance spectroscopy platform was used to assess 123 parameters. Principal component analysis of the serum metabolome demonstrated distinct clustering among individuals in each group, with the cluster of HLEA being broader compared to the other groups, presumably due to the wider spectrum of hepatic diseases represented in these samples. While younger and older adult control dogs had very similar metabolomic patterns and clusters, there were changes in many metabolites in the hepatopathy groups. Higher phenylalanine and tyrosine concentrations, lower branched-chained amino acids (BCAAs) concentrations, and altered fatty acid parameters were seen in cPSS dogs compared to controls. In contrast, dogs with HLEA had increased concentrations of BCAAs, phenylalanine, and various lipoproteins. Machine learning based solely on the metabolomics data showed excellent group classification, potentially identifying a novel tool to differentiate hepatopathies. The observed changes in metabolic parameters could provide invaluable insight into the pathophysiology, diagnosis, and prognosis of hepatopathies.
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spelling pubmed-89646952022-03-30 Metabolomic serum abnormalities in dogs with hepatopathies Imbery, Carolin A. Dieterle, Frank Ottka, Claudia Weber, Corinna Schlotterbeck, Götz Müller, Elisabeth Lohi, Hannes Giger, Urs Sci Rep Article Hepatopathies can cause major metabolic abnormalities in humans and animals. This study examined differences in serum metabolomic parameters and patterns in left-over serum samples from dogs with either congenital portosystemic shunts (cPSS, n = 24) or high serum liver enzyme activities (HLEA, n = 25) compared to control dogs (n = 64). A validated targeted proton nuclear magnetic resonance spectroscopy platform was used to assess 123 parameters. Principal component analysis of the serum metabolome demonstrated distinct clustering among individuals in each group, with the cluster of HLEA being broader compared to the other groups, presumably due to the wider spectrum of hepatic diseases represented in these samples. While younger and older adult control dogs had very similar metabolomic patterns and clusters, there were changes in many metabolites in the hepatopathy groups. Higher phenylalanine and tyrosine concentrations, lower branched-chained amino acids (BCAAs) concentrations, and altered fatty acid parameters were seen in cPSS dogs compared to controls. In contrast, dogs with HLEA had increased concentrations of BCAAs, phenylalanine, and various lipoproteins. Machine learning based solely on the metabolomics data showed excellent group classification, potentially identifying a novel tool to differentiate hepatopathies. The observed changes in metabolic parameters could provide invaluable insight into the pathophysiology, diagnosis, and prognosis of hepatopathies. Nature Publishing Group UK 2022-03-29 /pmc/articles/PMC8964695/ /pubmed/35351920 http://dx.doi.org/10.1038/s41598-022-09056-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Imbery, Carolin A.
Dieterle, Frank
Ottka, Claudia
Weber, Corinna
Schlotterbeck, Götz
Müller, Elisabeth
Lohi, Hannes
Giger, Urs
Metabolomic serum abnormalities in dogs with hepatopathies
title Metabolomic serum abnormalities in dogs with hepatopathies
title_full Metabolomic serum abnormalities in dogs with hepatopathies
title_fullStr Metabolomic serum abnormalities in dogs with hepatopathies
title_full_unstemmed Metabolomic serum abnormalities in dogs with hepatopathies
title_short Metabolomic serum abnormalities in dogs with hepatopathies
title_sort metabolomic serum abnormalities in dogs with hepatopathies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964695/
https://www.ncbi.nlm.nih.gov/pubmed/35351920
http://dx.doi.org/10.1038/s41598-022-09056-5
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