Highly efficient prime editing by introducing same-sense mutations in pegRNA or stabilizing its structure

Prime editor (PE), which is developed by combining Cas9 nickase and an engineered reverse transcriptase, can mediate all twelve types of base substitutions and small insertions or deletions in living cells but its efficiency remains low. Here, we develop spegRNA by introducing same-sense mutations a...

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Detalles Bibliográficos
Autores principales: Li, Xiaosa, Zhou, Lina, Gao, Bao-Qing, Li, Guangye, Wang, Xiao, Wang, Ying, Wei, Jia, Han, Wenyan, Wang, Zixian, Li, Jifang, Gao, Runze, Zhu, Junjie, Xu, Wenchao, Wu, Jing, Yang, Bei, Sun, Xiaodong, Yang, Li, Chen, Jia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964725/
https://www.ncbi.nlm.nih.gov/pubmed/35351879
http://dx.doi.org/10.1038/s41467-022-29339-9

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