Cargando…

Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction

Low back pain is one of the most serious public health problems worldwide and the major clinical manifestation of intervertebral disc degeneration (IVDD). The key pathological change during IVDD is dysfunction of the annulus fibrosus (AF). However, due to the lack of an in-depth understanding of AF...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Han, Wang, Di, Luo, Beier, Wang, Dong, Jia, Haoruo, Peng, Pandi, Shang, Qiliang, Mao, Jianxin, Gao, Chu, Peng, Ye, Gan, Lu, Du, Junjie, Luo, Zhuojing, Yang, Liu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964821/
https://www.ncbi.nlm.nih.gov/pubmed/35386822
http://dx.doi.org/10.1016/j.bioactmat.2022.01.040
_version_ 1784678304189513728
author Wang, Han
Wang, Di
Luo, Beier
Wang, Dong
Jia, Haoruo
Peng, Pandi
Shang, Qiliang
Mao, Jianxin
Gao, Chu
Peng, Ye
Gan, Lu
Du, Junjie
Luo, Zhuojing
Yang, Liu
author_facet Wang, Han
Wang, Di
Luo, Beier
Wang, Dong
Jia, Haoruo
Peng, Pandi
Shang, Qiliang
Mao, Jianxin
Gao, Chu
Peng, Ye
Gan, Lu
Du, Junjie
Luo, Zhuojing
Yang, Liu
author_sort Wang, Han
collection PubMed
description Low back pain is one of the most serious public health problems worldwide and the major clinical manifestation of intervertebral disc degeneration (IVDD). The key pathological change during IVDD is dysfunction of the annulus fibrosus (AF). However, due to the lack of an in-depth understanding of AF biology, the methods to reconstruct the AF are very limited. In this study, the mice AF cell atlas were decoded by single-cell RNA sequencing to provide a guide for AF reconstruction. The results first identify a new population of AF cells, fibrochondrocyte-like AF cells, which synthesize both collagen I and collagen II and are potential functional cells for AF reconstruction. According to the dual features of the AF extracellular matrix, a composite hydrogel based on the acylation of methacrylated silk fibroin with methacrylated hyaluronic acid was produced. To obtain the ability to stimulate differentiation, the composite hydrogels were combined with a fibrochondrocyte-inducing supplement. Finally, reconstruction of the AF defects, by the novel AF stem cell-loaded composite hydrogel, could be observed, its amount of chondroid matrices recovered to 31.7% of AF aera which is significantly higher than that in other control groups. In summary, this study decodes the AF cell atlas, based on which a novel strategy for AF reconstruction is proposed.
format Online
Article
Text
id pubmed-8964821
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher KeAi Publishing
record_format MEDLINE/PubMed
spelling pubmed-89648212022-04-05 Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction Wang, Han Wang, Di Luo, Beier Wang, Dong Jia, Haoruo Peng, Pandi Shang, Qiliang Mao, Jianxin Gao, Chu Peng, Ye Gan, Lu Du, Junjie Luo, Zhuojing Yang, Liu Bioact Mater Article Low back pain is one of the most serious public health problems worldwide and the major clinical manifestation of intervertebral disc degeneration (IVDD). The key pathological change during IVDD is dysfunction of the annulus fibrosus (AF). However, due to the lack of an in-depth understanding of AF biology, the methods to reconstruct the AF are very limited. In this study, the mice AF cell atlas were decoded by single-cell RNA sequencing to provide a guide for AF reconstruction. The results first identify a new population of AF cells, fibrochondrocyte-like AF cells, which synthesize both collagen I and collagen II and are potential functional cells for AF reconstruction. According to the dual features of the AF extracellular matrix, a composite hydrogel based on the acylation of methacrylated silk fibroin with methacrylated hyaluronic acid was produced. To obtain the ability to stimulate differentiation, the composite hydrogels were combined with a fibrochondrocyte-inducing supplement. Finally, reconstruction of the AF defects, by the novel AF stem cell-loaded composite hydrogel, could be observed, its amount of chondroid matrices recovered to 31.7% of AF aera which is significantly higher than that in other control groups. In summary, this study decodes the AF cell atlas, based on which a novel strategy for AF reconstruction is proposed. KeAi Publishing 2022-02-03 /pmc/articles/PMC8964821/ /pubmed/35386822 http://dx.doi.org/10.1016/j.bioactmat.2022.01.040 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Wang, Han
Wang, Di
Luo, Beier
Wang, Dong
Jia, Haoruo
Peng, Pandi
Shang, Qiliang
Mao, Jianxin
Gao, Chu
Peng, Ye
Gan, Lu
Du, Junjie
Luo, Zhuojing
Yang, Liu
Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction
title Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction
title_full Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction
title_fullStr Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction
title_full_unstemmed Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction
title_short Decoding the annulus fibrosus cell atlas by scRNA-seq to develop an inducible composite hydrogel: A novel strategy for disc reconstruction
title_sort decoding the annulus fibrosus cell atlas by scrna-seq to develop an inducible composite hydrogel: a novel strategy for disc reconstruction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964821/
https://www.ncbi.nlm.nih.gov/pubmed/35386822
http://dx.doi.org/10.1016/j.bioactmat.2022.01.040
work_keys_str_mv AT wanghan decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT wangdi decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT luobeier decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT wangdong decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT jiahaoruo decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT pengpandi decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT shangqiliang decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT maojianxin decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT gaochu decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT pengye decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT ganlu decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT dujunjie decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT luozhuojing decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction
AT yangliu decodingtheannulusfibrosuscellatlasbyscrnaseqtodevelopaninduciblecompositehydrogelanovelstrategyfordiscreconstruction