Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser

BACKGROUND: Thermo-alkali stable xylanases were purified from the extracellular broth of newly isolated Bacillus licheniformis strain produced in a 5-L stirred-tank bioreactor with wheat bran as a carbon source. RESULTS: A high degree of purity was achieved using size exclusion chromatography result...

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Detalles Bibliográficos
Autores principales: Malhotra, Girisha, Chapadgaonkar, Shilpa S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964892/
https://www.ncbi.nlm.nih.gov/pubmed/35348915
http://dx.doi.org/10.1186/s43141-022-00333-4
Descripción
Sumario:BACKGROUND: Thermo-alkali stable xylanases were purified from the extracellular broth of newly isolated Bacillus licheniformis strain produced in a 5-L stirred-tank bioreactor with wheat bran as a carbon source. RESULTS: A high degree of purity was achieved using size exclusion chromatography resulting in 16-fold purification and 69% recovery for fraction 5 which had the highest activity. The recovery obtained after pooling fractions 5 and 6 was 99%. The K(m) value of xylanase was calculated as 0.05 mM, and V(max) was 125 μmol/min/mg protein. CONCLUSION: Purified xylanase had a high thermal and pH stability. Xylanase was found to be suitable for application in the de-inking of paper and for saccharification of lignocellulosic waste biomass.

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