Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser

BACKGROUND: Thermo-alkali stable xylanases were purified from the extracellular broth of newly isolated Bacillus licheniformis strain produced in a 5-L stirred-tank bioreactor with wheat bran as a carbon source. RESULTS: A high degree of purity was achieved using size exclusion chromatography result...

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Autores principales: Malhotra, Girisha, Chapadgaonkar, Shilpa S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964892/
https://www.ncbi.nlm.nih.gov/pubmed/35348915
http://dx.doi.org/10.1186/s43141-022-00333-4
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author Malhotra, Girisha
Chapadgaonkar, Shilpa S.
author_facet Malhotra, Girisha
Chapadgaonkar, Shilpa S.
author_sort Malhotra, Girisha
collection PubMed
description BACKGROUND: Thermo-alkali stable xylanases were purified from the extracellular broth of newly isolated Bacillus licheniformis strain produced in a 5-L stirred-tank bioreactor with wheat bran as a carbon source. RESULTS: A high degree of purity was achieved using size exclusion chromatography resulting in 16-fold purification and 69% recovery for fraction 5 which had the highest activity. The recovery obtained after pooling fractions 5 and 6 was 99%. The K(m) value of xylanase was calculated as 0.05 mM, and V(max) was 125 μmol/min/mg protein. CONCLUSION: Purified xylanase had a high thermal and pH stability. Xylanase was found to be suitable for application in the de-inking of paper and for saccharification of lignocellulosic waste biomass.
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spelling pubmed-89648922022-04-12 Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser Malhotra, Girisha Chapadgaonkar, Shilpa S. J Genet Eng Biotechnol Research BACKGROUND: Thermo-alkali stable xylanases were purified from the extracellular broth of newly isolated Bacillus licheniformis strain produced in a 5-L stirred-tank bioreactor with wheat bran as a carbon source. RESULTS: A high degree of purity was achieved using size exclusion chromatography resulting in 16-fold purification and 69% recovery for fraction 5 which had the highest activity. The recovery obtained after pooling fractions 5 and 6 was 99%. The K(m) value of xylanase was calculated as 0.05 mM, and V(max) was 125 μmol/min/mg protein. CONCLUSION: Purified xylanase had a high thermal and pH stability. Xylanase was found to be suitable for application in the de-inking of paper and for saccharification of lignocellulosic waste biomass. Springer Berlin Heidelberg 2022-03-29 /pmc/articles/PMC8964892/ /pubmed/35348915 http://dx.doi.org/10.1186/s43141-022-00333-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Malhotra, Girisha
Chapadgaonkar, Shilpa S.
Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser
title Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser
title_full Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser
title_fullStr Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser
title_full_unstemmed Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser
title_short Partial purification and characterization of a thermostable xylanase from Bacillus licheniformis isolated from hot water geyser
title_sort partial purification and characterization of a thermostable xylanase from bacillus licheniformis isolated from hot water geyser
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964892/
https://www.ncbi.nlm.nih.gov/pubmed/35348915
http://dx.doi.org/10.1186/s43141-022-00333-4
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