The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection

Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS(2) (QD-MB @MoS(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRN...

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Detalles Bibliográficos
Autores principales: Wang, Jing Jing, Liu, Ying, Ding, Zhou, Zhang, Le, Han, Caiqin, Yan, Changchun, Amador, Eric, Yuan, Liqin, Wu, Ying, Song, Chunyuan, Chen, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964961/
https://www.ncbi.nlm.nih.gov/pubmed/35386454
http://dx.doi.org/10.1016/j.bioactmat.2021.12.036
Descripción
Sumario:Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS(2) (QD-MB @MoS(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.

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