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The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection
Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS(2) (QD-MB @MoS(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRN...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964961/ https://www.ncbi.nlm.nih.gov/pubmed/35386454 http://dx.doi.org/10.1016/j.bioactmat.2021.12.036 |
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author | Wang, Jing Jing Liu, Ying Ding, Zhou Zhang, Le Han, Caiqin Yan, Changchun Amador, Eric Yuan, Liqin Wu, Ying Song, Chunyuan Liu, Ying Chen, Wei |
author_facet | Wang, Jing Jing Liu, Ying Ding, Zhou Zhang, Le Han, Caiqin Yan, Changchun Amador, Eric Yuan, Liqin Wu, Ying Song, Chunyuan Liu, Ying Chen, Wei |
author_sort | Wang, Jing Jing |
collection | PubMed |
description | Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS(2) (QD-MB @MoS(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis. |
format | Online Article Text |
id | pubmed-8964961 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-89649612022-04-05 The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection Wang, Jing Jing Liu, Ying Ding, Zhou Zhang, Le Han, Caiqin Yan, Changchun Amador, Eric Yuan, Liqin Wu, Ying Song, Chunyuan Liu, Ying Chen, Wei Bioact Mater Article Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS(2) (QD-MB @MoS(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis. KeAi Publishing 2022-01-07 /pmc/articles/PMC8964961/ /pubmed/35386454 http://dx.doi.org/10.1016/j.bioactmat.2021.12.036 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Jing Jing Liu, Ying Ding, Zhou Zhang, Le Han, Caiqin Yan, Changchun Amador, Eric Yuan, Liqin Wu, Ying Song, Chunyuan Liu, Ying Chen, Wei The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection |
title | The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection |
title_full | The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection |
title_fullStr | The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection |
title_full_unstemmed | The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection |
title_short | The exploration of quantum dot-molecular beacon based MoS(2) fluorescence probing for myeloma-related Mirnas detection |
title_sort | exploration of quantum dot-molecular beacon based mos(2) fluorescence probing for myeloma-related mirnas detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8964961/ https://www.ncbi.nlm.nih.gov/pubmed/35386454 http://dx.doi.org/10.1016/j.bioactmat.2021.12.036 |
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