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Antibiofilm and immunomodulatory resorbable nanofibrous filing for dental pulp regenerative procedures

Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry. Here, different scaffolds were tested for use during the pulp revascularization process, including poly(vinyl alcohol)-PVA hydrogels or resins, collagen...

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Detalles Bibliográficos
Autores principales: Gonçalves da Costa Sousa, Mauricio, Conceição de Almeida, Gabriela, Martins Mota, Danilo César, Andrade da Costa, Rosiane, Dias, Simoni Campos, Limberger, Samuel Nunes, Ko, Frank, Lin, Li Ting, Haney, Evan F., Etayash, Hashem, Baquir, Beverlie, Trimble, Michael J., Shen, Ya, Su, Zheng, Haapasalo, Markus, Pletzer, Daniel, Chaves de Souza, Letícia, Schuindt Teixeira, Gláucia, Silva, Renato M., Hancock, Robert E.W., Franco, Octavio Luiz, Berto Rezende, Taia Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965695/
https://www.ncbi.nlm.nih.gov/pubmed/35386316
http://dx.doi.org/10.1016/j.bioactmat.2022.01.027
Descripción
Sumario:Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry. Here, different scaffolds were tested for use during the pulp revascularization process, including poly(vinyl alcohol)-PVA hydrogels or resins, collagen hydrogels and poly(vinyl alcohol) PVA/Chitosan (PVA/CS) nanofibers. Based on time to degradation (21 days), nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002 (each at 50 mg/g). Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis, Staphylococcus aureus and a multispecies oral biofilm, besides anti-inflammatory activities. The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation, when compared to empty teeth fragments. Thus, we designed a strong antimicrobial, immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.