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The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors

Convalescent plasma therapy has been described as an attractive approach to treat critically ill patients with COVID-19 (Coronavirus disease 2019). The selection of convalescent plasma donors (CPD) is commonly based on neutralizing antibody titer. A better understanding of the quality of immune resp...

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Autores principales: Kroemer, Marie, Boullerot, Laura, Ramseyer, Mélanie, Spehner, Laurie, Barisien, Christophe, Gravelin, Eleonore, Renaudin, Adeline, Cognasse, Fabrice, Gallian, Pierre, Hermine, Olivier, Lacombe, Karine, Tiberghien, Pierre, Adotévi, Olivier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965758/
https://www.ncbi.nlm.nih.gov/pubmed/35372242
http://dx.doi.org/10.3389/fpubh.2022.816848
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author Kroemer, Marie
Boullerot, Laura
Ramseyer, Mélanie
Spehner, Laurie
Barisien, Christophe
Gravelin, Eleonore
Renaudin, Adeline
Cognasse, Fabrice
Gallian, Pierre
Hermine, Olivier
Lacombe, Karine
Tiberghien, Pierre
Adotévi, Olivier
author_facet Kroemer, Marie
Boullerot, Laura
Ramseyer, Mélanie
Spehner, Laurie
Barisien, Christophe
Gravelin, Eleonore
Renaudin, Adeline
Cognasse, Fabrice
Gallian, Pierre
Hermine, Olivier
Lacombe, Karine
Tiberghien, Pierre
Adotévi, Olivier
author_sort Kroemer, Marie
collection PubMed
description Convalescent plasma therapy has been described as an attractive approach to treat critically ill patients with COVID-19 (Coronavirus disease 2019). The selection of convalescent plasma donors (CPD) is commonly based on neutralizing antibody titer. A better understanding of the quality of immune responses following COVID-19 will enable the optimization of convalescent donors' selection in convalescent plasma programs. The involvement of SARS-CoV-2 specific T cells in the induction and persistence of high affinity anti-SARS-CoV-2 neutralizing antibody is still poorly investigated. In this study, 115 CPD who presented SARS-CoV-2 and who were eligible for plasma donation were included. Comprehensive analysis of T cells together with humoral responses were performed in regards of sex, age and blood group type. High frequency of T cell responses against SARS-CoV-2 related protein such as spike glycoprotein (80.0%), nucleocapsid (NCAP) (70.4%) and membrane protein (VME1) (74.8%) were detected in CPD by ex vivo IFN-γ and TNF-α ELISpot assays. Among CPD responders, most exhibited poly-specific T cell responses (75%) defined by the ability to mount responses against at least two SARS-CoV-2 antigens. We found a positive correlation between the magnitude and the poly-specificity of anti-SARS-CoV-2 T cell responses in CPD. Notably, both the magnitude and poly-specificity of SARS-CoV-2 T cell responses were highly correlated with neutralizing antibody titer in CPD. The present study highlights that the poly-specificity and strength of SARS-CoV-2 specific T cell responses predicts neutralizing antibody titer following COVID-19. These observations show the interest to combine T cell assays and antibody titer for the selection of CPD and to a latter extend to assess COVID-19 vaccine efficacy in at-risk patients.
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spelling pubmed-89657582022-03-31 The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors Kroemer, Marie Boullerot, Laura Ramseyer, Mélanie Spehner, Laurie Barisien, Christophe Gravelin, Eleonore Renaudin, Adeline Cognasse, Fabrice Gallian, Pierre Hermine, Olivier Lacombe, Karine Tiberghien, Pierre Adotévi, Olivier Front Public Health Public Health Convalescent plasma therapy has been described as an attractive approach to treat critically ill patients with COVID-19 (Coronavirus disease 2019). The selection of convalescent plasma donors (CPD) is commonly based on neutralizing antibody titer. A better understanding of the quality of immune responses following COVID-19 will enable the optimization of convalescent donors' selection in convalescent plasma programs. The involvement of SARS-CoV-2 specific T cells in the induction and persistence of high affinity anti-SARS-CoV-2 neutralizing antibody is still poorly investigated. In this study, 115 CPD who presented SARS-CoV-2 and who were eligible for plasma donation were included. Comprehensive analysis of T cells together with humoral responses were performed in regards of sex, age and blood group type. High frequency of T cell responses against SARS-CoV-2 related protein such as spike glycoprotein (80.0%), nucleocapsid (NCAP) (70.4%) and membrane protein (VME1) (74.8%) were detected in CPD by ex vivo IFN-γ and TNF-α ELISpot assays. Among CPD responders, most exhibited poly-specific T cell responses (75%) defined by the ability to mount responses against at least two SARS-CoV-2 antigens. We found a positive correlation between the magnitude and the poly-specificity of anti-SARS-CoV-2 T cell responses in CPD. Notably, both the magnitude and poly-specificity of SARS-CoV-2 T cell responses were highly correlated with neutralizing antibody titer in CPD. The present study highlights that the poly-specificity and strength of SARS-CoV-2 specific T cell responses predicts neutralizing antibody titer following COVID-19. These observations show the interest to combine T cell assays and antibody titer for the selection of CPD and to a latter extend to assess COVID-19 vaccine efficacy in at-risk patients. Frontiers Media S.A. 2022-03-16 /pmc/articles/PMC8965758/ /pubmed/35372242 http://dx.doi.org/10.3389/fpubh.2022.816848 Text en Copyright © 2022 Kroemer, Boullerot, Ramseyer, Spehner, Barisien, Gravelin, Renaudin, Cognasse, Gallian, Hermine, Lacombe, Tiberghien and Adotévi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Public Health
Kroemer, Marie
Boullerot, Laura
Ramseyer, Mélanie
Spehner, Laurie
Barisien, Christophe
Gravelin, Eleonore
Renaudin, Adeline
Cognasse, Fabrice
Gallian, Pierre
Hermine, Olivier
Lacombe, Karine
Tiberghien, Pierre
Adotévi, Olivier
The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors
title The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors
title_full The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors
title_fullStr The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors
title_full_unstemmed The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors
title_short The Quality of Anti-SARS-CoV-2 T Cell Responses Predicts the Neutralizing Antibody Titer in Convalescent Plasma Donors
title_sort quality of anti-sars-cov-2 t cell responses predicts the neutralizing antibody titer in convalescent plasma donors
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965758/
https://www.ncbi.nlm.nih.gov/pubmed/35372242
http://dx.doi.org/10.3389/fpubh.2022.816848
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