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Osteogenic lithium-doped brushite cements for bone regeneration
This study investigated the osteogenic performance of new brushite cements obtained from Li(+)-doped β-tricalcium phosphate as a promising strategy for bone regeneration. Lithium (Li(+)) is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells (hASCs) a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965853/ https://www.ncbi.nlm.nih.gov/pubmed/35415287 http://dx.doi.org/10.1016/j.bioactmat.2021.12.025 |
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author | Hurle, K. Maia, F.R. Ribeiro, V.P. Pina, S. Oliveira, J.M. Goetz-Neunhoeffer, F. Reis, R.L. |
author_facet | Hurle, K. Maia, F.R. Ribeiro, V.P. Pina, S. Oliveira, J.M. Goetz-Neunhoeffer, F. Reis, R.L. |
author_sort | Hurle, K. |
collection | PubMed |
description | This study investigated the osteogenic performance of new brushite cements obtained from Li(+)-doped β-tricalcium phosphate as a promising strategy for bone regeneration. Lithium (Li(+)) is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells (hASCs) and the osteogenic differentiation-related gene expression, essential for osteogenesis. In-situ X-ray diffraction (XRD) and in-situ(1)H nuclear magnetic resonance ((1)H NMR) measurements proved the precipitation of brushite, as main phase, and monetite, indicating that Li(+) favored the formation of monetite under certain conditions. Li(+) was detected in the remaining pore solution in significant amounts after the completion of hydration. Isothermal calorimetry results showed an accelerating effect of Li(+), especially for low concentration of the setting retarder (phytic acid). A decrease of initial and final setting times with increasing amount of Li(+) was detected and setting times could be well adjusted by varying the setting retarder concentration. The cements presented compressive mechanical strength within the ranges reported for cancellous bone. In vitro assays using hASCs showed normal metabolic and proliferative levels. The immunodetection and gene expression profile of osteogenic-related markers highlight the incorporation of Li(+) for increasing the in vivo bone density. The osteogenic potential of Li-doped brushite cements may be recommended for further research on bone defect repair strategies. |
format | Online Article Text |
id | pubmed-8965853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-89658532022-04-11 Osteogenic lithium-doped brushite cements for bone regeneration Hurle, K. Maia, F.R. Ribeiro, V.P. Pina, S. Oliveira, J.M. Goetz-Neunhoeffer, F. Reis, R.L. Bioact Mater Article This study investigated the osteogenic performance of new brushite cements obtained from Li(+)-doped β-tricalcium phosphate as a promising strategy for bone regeneration. Lithium (Li(+)) is a promising trace element to encourage the migration and proliferation of adipose-derived stem cells (hASCs) and the osteogenic differentiation-related gene expression, essential for osteogenesis. In-situ X-ray diffraction (XRD) and in-situ(1)H nuclear magnetic resonance ((1)H NMR) measurements proved the precipitation of brushite, as main phase, and monetite, indicating that Li(+) favored the formation of monetite under certain conditions. Li(+) was detected in the remaining pore solution in significant amounts after the completion of hydration. Isothermal calorimetry results showed an accelerating effect of Li(+), especially for low concentration of the setting retarder (phytic acid). A decrease of initial and final setting times with increasing amount of Li(+) was detected and setting times could be well adjusted by varying the setting retarder concentration. The cements presented compressive mechanical strength within the ranges reported for cancellous bone. In vitro assays using hASCs showed normal metabolic and proliferative levels. The immunodetection and gene expression profile of osteogenic-related markers highlight the incorporation of Li(+) for increasing the in vivo bone density. The osteogenic potential of Li-doped brushite cements may be recommended for further research on bone defect repair strategies. KeAi Publishing 2021-12-31 /pmc/articles/PMC8965853/ /pubmed/35415287 http://dx.doi.org/10.1016/j.bioactmat.2021.12.025 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Hurle, K. Maia, F.R. Ribeiro, V.P. Pina, S. Oliveira, J.M. Goetz-Neunhoeffer, F. Reis, R.L. Osteogenic lithium-doped brushite cements for bone regeneration |
title | Osteogenic lithium-doped brushite cements for bone regeneration |
title_full | Osteogenic lithium-doped brushite cements for bone regeneration |
title_fullStr | Osteogenic lithium-doped brushite cements for bone regeneration |
title_full_unstemmed | Osteogenic lithium-doped brushite cements for bone regeneration |
title_short | Osteogenic lithium-doped brushite cements for bone regeneration |
title_sort | osteogenic lithium-doped brushite cements for bone regeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965853/ https://www.ncbi.nlm.nih.gov/pubmed/35415287 http://dx.doi.org/10.1016/j.bioactmat.2021.12.025 |
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