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A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo

To control capillary bleeding, surgeons may use absorbable hemostatic agents, such as Surgicel® and TachoSil®. Due to their slow resorption, their persistence in situ can have a negative impact on tissue repair in the resected organ. To avoid complications and obtain a hemostatic agent that promotes...

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Autores principales: Ponsen, Anne-Charlotte, Proust, Richard, Soave, Sabrina, Mercier-Nomé, Françoise, Garcin, Isabelle, Combettes, Laurent, Lataillade, Jean-Jacques, Uzan, Georges
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965972/
https://www.ncbi.nlm.nih.gov/pubmed/35415309
http://dx.doi.org/10.1016/j.bioactmat.2022.01.049
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author Ponsen, Anne-Charlotte
Proust, Richard
Soave, Sabrina
Mercier-Nomé, Françoise
Garcin, Isabelle
Combettes, Laurent
Lataillade, Jean-Jacques
Uzan, Georges
author_facet Ponsen, Anne-Charlotte
Proust, Richard
Soave, Sabrina
Mercier-Nomé, Françoise
Garcin, Isabelle
Combettes, Laurent
Lataillade, Jean-Jacques
Uzan, Georges
author_sort Ponsen, Anne-Charlotte
collection PubMed
description To control capillary bleeding, surgeons may use absorbable hemostatic agents, such as Surgicel® and TachoSil®. Due to their slow resorption, their persistence in situ can have a negative impact on tissue repair in the resected organ. To avoid complications and obtain a hemostatic agent that promotes tissue repair, a zinc-supplemented calcium alginate compress was developed: HEMO-IONIC®. This compress is non-absorbable and is therefore removed once hemostasis has been achieved. After demonstrating the hemostatic efficacy and stability of the blood clot obtained with HEMO-IONIC, the impact of Surgicel, TachoSil, and HEMO-IONIC on cell activation and tissue repair were compared (i) in vitro on endothelial cells, which are essential to tissue repair, and (ii) in vivo in a mouse skin excision model. In vitro, only HEMO-IONIC maintained the phenotypic and functional properties of endothelial cells and induced their migration. In comparison, Surgicel was found to be highly cytotoxic, and TachoSil inhibited endothelial cell migration. In vivo, only HEMO-IONIC increased angiogenesis, the recruitment of cells essential to tissue repair (macrophages, fibroblasts, and epithelial cells), and accelerated maturation of the extracellular matrix. These results demonstrate that a zinc-supplemented calcium alginate, HEMO-IONIC, applied for 10 min at the end of surgery and then removed has a long-term positive effect on all phases of tissue repair.
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spelling pubmed-89659722022-04-11 A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo Ponsen, Anne-Charlotte Proust, Richard Soave, Sabrina Mercier-Nomé, Françoise Garcin, Isabelle Combettes, Laurent Lataillade, Jean-Jacques Uzan, Georges Bioact Mater Article To control capillary bleeding, surgeons may use absorbable hemostatic agents, such as Surgicel® and TachoSil®. Due to their slow resorption, their persistence in situ can have a negative impact on tissue repair in the resected organ. To avoid complications and obtain a hemostatic agent that promotes tissue repair, a zinc-supplemented calcium alginate compress was developed: HEMO-IONIC®. This compress is non-absorbable and is therefore removed once hemostasis has been achieved. After demonstrating the hemostatic efficacy and stability of the blood clot obtained with HEMO-IONIC, the impact of Surgicel, TachoSil, and HEMO-IONIC on cell activation and tissue repair were compared (i) in vitro on endothelial cells, which are essential to tissue repair, and (ii) in vivo in a mouse skin excision model. In vitro, only HEMO-IONIC maintained the phenotypic and functional properties of endothelial cells and induced their migration. In comparison, Surgicel was found to be highly cytotoxic, and TachoSil inhibited endothelial cell migration. In vivo, only HEMO-IONIC increased angiogenesis, the recruitment of cells essential to tissue repair (macrophages, fibroblasts, and epithelial cells), and accelerated maturation of the extracellular matrix. These results demonstrate that a zinc-supplemented calcium alginate, HEMO-IONIC, applied for 10 min at the end of surgery and then removed has a long-term positive effect on all phases of tissue repair. KeAi Publishing 2022-02-12 /pmc/articles/PMC8965972/ /pubmed/35415309 http://dx.doi.org/10.1016/j.bioactmat.2022.01.049 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ponsen, Anne-Charlotte
Proust, Richard
Soave, Sabrina
Mercier-Nomé, Françoise
Garcin, Isabelle
Combettes, Laurent
Lataillade, Jean-Jacques
Uzan, Georges
A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo
title A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo
title_full A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo
title_fullStr A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo
title_full_unstemmed A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo
title_short A new hemostatic agent composed of Zn(2+)-enriched Ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo
title_sort new hemostatic agent composed of zn(2+)-enriched ca(2+) alginate activates vascular endothelial cells in vitro and promotes tissue repair invivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8965972/
https://www.ncbi.nlm.nih.gov/pubmed/35415309
http://dx.doi.org/10.1016/j.bioactmat.2022.01.049
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