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Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice

Diabetic retinopathy (DR), a microvascular complication of diabetes mellitus, is the leading cause of vision loss in the working-age population worldwide. Unfortunately, current clinical treatments cannot completely prevent the occurrence and development of DR. Salidroside (Sal) is a medicinal suppl...

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Autores principales: Yao, Fei, Jiang, Xinyi, Qiu, Ling, Peng, Zixuan, Zheng, Wei, Ding, Lexi, Xia, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966089/
https://www.ncbi.nlm.nih.gov/pubmed/35370972
http://dx.doi.org/10.3389/fendo.2022.861452
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author Yao, Fei
Jiang, Xinyi
Qiu, Ling
Peng, Zixuan
Zheng, Wei
Ding, Lexi
Xia, Xiaobo
author_facet Yao, Fei
Jiang, Xinyi
Qiu, Ling
Peng, Zixuan
Zheng, Wei
Ding, Lexi
Xia, Xiaobo
author_sort Yao, Fei
collection PubMed
description Diabetic retinopathy (DR), a microvascular complication of diabetes mellitus, is the leading cause of vision loss in the working-age population worldwide. Unfortunately, current clinical treatments cannot completely prevent the occurrence and development of DR. Salidroside (Sal) is a medicinal supplement that has antioxidative and cytoprotective properties. This study aimed to investigate the therapeutic effect of Sal on DR. Briefly, Sal treatment was applied to wide-type mice and db/db mice (a widely used diabetic mice) at 25 mg/kg by oral gavage once daily from 8 weeks to 20 weeks. Mice’s bodyweight, blood glucose, total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein were recorded and analyzed. Retinal trypsin digestion and evans blue dye assay were used to detect retinal microvessel changes and function. Retinal glutathione and malondialdehyde content measurements were applied to assess retinal oxidative stress. Full-length transcriptome analysis was performed to explore the underlying mechanisms of Sal protection. Our results found that Sal treatment could successfully relieve blood glucose and blood lipid abnormalities, and reduce retinal oxidative stress level in diabetic mice. Also, Sal treatment repaired the abnormal transcriptome caused by diabetes, alleviated the microvascular lesion of the fundus in diabetic mice, and protected retinal normal barrier function. This study enriches the indications of Sal in the treatment of diabetic diseases, providing practical research ideas for the comprehensive preventions and treatments of DR.
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spelling pubmed-89660892022-03-31 Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice Yao, Fei Jiang, Xinyi Qiu, Ling Peng, Zixuan Zheng, Wei Ding, Lexi Xia, Xiaobo Front Endocrinol (Lausanne) Endocrinology Diabetic retinopathy (DR), a microvascular complication of diabetes mellitus, is the leading cause of vision loss in the working-age population worldwide. Unfortunately, current clinical treatments cannot completely prevent the occurrence and development of DR. Salidroside (Sal) is a medicinal supplement that has antioxidative and cytoprotective properties. This study aimed to investigate the therapeutic effect of Sal on DR. Briefly, Sal treatment was applied to wide-type mice and db/db mice (a widely used diabetic mice) at 25 mg/kg by oral gavage once daily from 8 weeks to 20 weeks. Mice’s bodyweight, blood glucose, total cholesterol, triglyceride, high density lipoprotein and low density lipoprotein were recorded and analyzed. Retinal trypsin digestion and evans blue dye assay were used to detect retinal microvessel changes and function. Retinal glutathione and malondialdehyde content measurements were applied to assess retinal oxidative stress. Full-length transcriptome analysis was performed to explore the underlying mechanisms of Sal protection. Our results found that Sal treatment could successfully relieve blood glucose and blood lipid abnormalities, and reduce retinal oxidative stress level in diabetic mice. Also, Sal treatment repaired the abnormal transcriptome caused by diabetes, alleviated the microvascular lesion of the fundus in diabetic mice, and protected retinal normal barrier function. This study enriches the indications of Sal in the treatment of diabetic diseases, providing practical research ideas for the comprehensive preventions and treatments of DR. Frontiers Media S.A. 2022-03-16 /pmc/articles/PMC8966089/ /pubmed/35370972 http://dx.doi.org/10.3389/fendo.2022.861452 Text en Copyright © 2022 Yao, Jiang, Qiu, Peng, Zheng, Ding and Xia https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Yao, Fei
Jiang, Xinyi
Qiu, Ling
Peng, Zixuan
Zheng, Wei
Ding, Lexi
Xia, Xiaobo
Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice
title Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice
title_full Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice
title_fullStr Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice
title_full_unstemmed Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice
title_short Long-Term Oral Administration of Salidroside Alleviates Diabetic Retinopathy in db/db Mice
title_sort long-term oral administration of salidroside alleviates diabetic retinopathy in db/db mice
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966089/
https://www.ncbi.nlm.nih.gov/pubmed/35370972
http://dx.doi.org/10.3389/fendo.2022.861452
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