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SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines

Global environmental changes with more extreme episodes of heat waves are major threats to agricultural productivity. Heat stress in spring affects the reproductive stage of maize, resulting in tassel blast, pollen abortion, poor pollination, reduced seed set, barren ears and ultimately yield loss....

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Autores principales: Ahmed, Zubair, Khalid, Maria, Ghafoor, Abdul, Shah, Muhammad Kausar Nawaz, Raja, Ghazala Kaukab, Rana, Rashid Mehmood, Mahmood, Tahir, Thompson, Addie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966704/
https://www.ncbi.nlm.nih.gov/pubmed/35368702
http://dx.doi.org/10.3389/fgene.2022.819849
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author Ahmed, Zubair
Khalid, Maria
Ghafoor, Abdul
Shah, Muhammad Kausar Nawaz
Raja, Ghazala Kaukab
Rana, Rashid Mehmood
Mahmood, Tahir
Thompson, Addie M.
author_facet Ahmed, Zubair
Khalid, Maria
Ghafoor, Abdul
Shah, Muhammad Kausar Nawaz
Raja, Ghazala Kaukab
Rana, Rashid Mehmood
Mahmood, Tahir
Thompson, Addie M.
author_sort Ahmed, Zubair
collection PubMed
description Global environmental changes with more extreme episodes of heat waves are major threats to agricultural productivity. Heat stress in spring affects the reproductive stage of maize, resulting in tassel blast, pollen abortion, poor pollination, reduced seed set, barren ears and ultimately yield loss. As an aneamophelous crop, maize has a propensity for pollen abortion under heat stress conditions. To overcome the existing challenges of heat stress and pollen abortion, this study utilized a broad genetic base of maize germplasm to identify superior alleles to be utilized in breeding programs. A panel of 375 inbred lines was morpho-physiologically screened under normal and heat stress conditions in two locations across two consecutive planting seasons, 2017 and 2018. The exposure of pollen to high temperature showed drastic decline in pollen germination percentage. The average pollen germination percentage (PGP) at 35 and 45°C was 40.3% and 9.7%, respectively, an average decline of 30.6%. A subset of 275 inbred lines were sequenced using tunable genotyping by sequencing, resulting in 170,098 single nucleotide polymorphisms (SNPs) after filtration. Genome wide association of PGP in a subset of 122 inbred lines resulted in ten SNPs associated with PGP35°C (p ≤ 10(−5)), nine with PGP45°C (p ≤ 10(−6)–10(−8)) and ten SNPs associated with PGP ratio (p ≤ 10(−5)). No SNPs were found to be in common across PGP traits. The number of favorable alleles possessed by each inbred line for PGP35°C, PGP45°C, and the PGP ratio ranged between 4 and 10, 3–13 and 5–13, respectively. In contrast, the number of negative alleles for these traits ranged between 2 and 8, 3–13 and 3–13, respectively. Genetic mapping of yield (adjusted weight per plant, AWP(−1)) and flowering time (anthesis-silking interval, ASI) in 275 lines revealed five common SNPs: three shared for AWP(−1) between normal and heat stress conditions, one for ASI between conditions, and one SNP, CM007648.1-86615409, was associated with both ASI and AWP(−1). Variety selection can be performed based on these favorable alleles for various traits. These marker trait associations identified in the diversity panel can be utilized in breeding programs to improve heat stress tolerance in maize.
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spelling pubmed-89667042022-03-31 SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines Ahmed, Zubair Khalid, Maria Ghafoor, Abdul Shah, Muhammad Kausar Nawaz Raja, Ghazala Kaukab Rana, Rashid Mehmood Mahmood, Tahir Thompson, Addie M. Front Genet Genetics Global environmental changes with more extreme episodes of heat waves are major threats to agricultural productivity. Heat stress in spring affects the reproductive stage of maize, resulting in tassel blast, pollen abortion, poor pollination, reduced seed set, barren ears and ultimately yield loss. As an aneamophelous crop, maize has a propensity for pollen abortion under heat stress conditions. To overcome the existing challenges of heat stress and pollen abortion, this study utilized a broad genetic base of maize germplasm to identify superior alleles to be utilized in breeding programs. A panel of 375 inbred lines was morpho-physiologically screened under normal and heat stress conditions in two locations across two consecutive planting seasons, 2017 and 2018. The exposure of pollen to high temperature showed drastic decline in pollen germination percentage. The average pollen germination percentage (PGP) at 35 and 45°C was 40.3% and 9.7%, respectively, an average decline of 30.6%. A subset of 275 inbred lines were sequenced using tunable genotyping by sequencing, resulting in 170,098 single nucleotide polymorphisms (SNPs) after filtration. Genome wide association of PGP in a subset of 122 inbred lines resulted in ten SNPs associated with PGP35°C (p ≤ 10(−5)), nine with PGP45°C (p ≤ 10(−6)–10(−8)) and ten SNPs associated with PGP ratio (p ≤ 10(−5)). No SNPs were found to be in common across PGP traits. The number of favorable alleles possessed by each inbred line for PGP35°C, PGP45°C, and the PGP ratio ranged between 4 and 10, 3–13 and 5–13, respectively. In contrast, the number of negative alleles for these traits ranged between 2 and 8, 3–13 and 3–13, respectively. Genetic mapping of yield (adjusted weight per plant, AWP(−1)) and flowering time (anthesis-silking interval, ASI) in 275 lines revealed five common SNPs: three shared for AWP(−1) between normal and heat stress conditions, one for ASI between conditions, and one SNP, CM007648.1-86615409, was associated with both ASI and AWP(−1). Variety selection can be performed based on these favorable alleles for various traits. These marker trait associations identified in the diversity panel can be utilized in breeding programs to improve heat stress tolerance in maize. Frontiers Media S.A. 2022-03-16 /pmc/articles/PMC8966704/ /pubmed/35368702 http://dx.doi.org/10.3389/fgene.2022.819849 Text en Copyright © 2022 Ahmed, Khalid, Ghafoor, Shah, Raja, Rana, Mahmood and Thompson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Ahmed, Zubair
Khalid, Maria
Ghafoor, Abdul
Shah, Muhammad Kausar Nawaz
Raja, Ghazala Kaukab
Rana, Rashid Mehmood
Mahmood, Tahir
Thompson, Addie M.
SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines
title SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines
title_full SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines
title_fullStr SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines
title_full_unstemmed SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines
title_short SNP-Based Genome-Wide Association Mapping of Pollen Viability Under Heat Stress in Tropical Zea mays L. Inbred Lines
title_sort snp-based genome-wide association mapping of pollen viability under heat stress in tropical zea mays l. inbred lines
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8966704/
https://www.ncbi.nlm.nih.gov/pubmed/35368702
http://dx.doi.org/10.3389/fgene.2022.819849
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